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Antibody Phage Display

by Robert Aitken

Since its introduction almost 20 years ago, phage display technology has revolutionized approaches to the analysis of biomedical problems, quickly impacting the fields of immunology, cell biology, biotechnology, pharmacology, and drug discovery. In Antibody Phage Display: Methods and Protocols, Second Edition, expert researchers explore the latest in this cutting-edge technology, providing an invaluable resource that will guide readers in the design and execution of experiments based around antibody phage display. Chapters present a wide range of methods of isolating recombinant antibodies from phage display libraries, examine how the targets recognized by antibodies of interest can be identified, discuss the identification and exploitation of antibodies that can enter cells and bind to cytosolic targets, and include novel approaches to the expression of recombinant antibodies. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Detailed and innovative, Antibody Phage Display: Methods and Protocols, Second Edition is a critical handbook on phage display technology which is certain to stimulate the reader's imagination as much as it will guide future practice in the laboratory.

Antibody Phage Display

by Robert Aitken Philippa M. O’brien

This comprehensive collection of established antibody phage display protocols features authoritative guidance that will enable the nonspecialist successfully to carry them out. Coverage spans the construction of antibody libraries, the selection of antibody clones with the desired properties, and their modification, expression, and purification. Comprehensive and highly practical, Antibody Phage Display: Methods and Protocols provides biochemists, molecular biologists, and immunologists with a gold-standard reference guide to the successful isolation, modification, and expression of recombinant antibodies using today's powerful phage display technology.

Encouraging Words

by Robert Aitken

Encouraging Words is a collection of short talks and brief essays that Aitken Rashi has offered his students at meditation retreats during the past two decades. They are arranged according to themes central to all spiritual seekers -- attention, emptiness, coming and going, diligence, death and the afterlife, the sacred self, and the moral path. Aitken provides guidance on pursuing religious practice in a lay context, "re-casting the Dharma to include women, jobs, and family." He also charts his own quest to develop a set of moral codes in keeping with Buddhism's basic precepts and honoring the enormous ethical challenges faced in the twentieth century.From the Trade Paperback edition.

The Long Road Turns to Joy

by Thich Nhat Hanh Robert Aitken

One of the few books focused completely on mindful walking and walking meditation. This revised edition of the best-selling title (nearly 80,000 copies sold to date) includes over 30 percent new material-including new walking meditation poems and practices-and provides a practical and inspirational introduction to this important practice. Written in Thich Nhat Hanh's clear and accessible style, Long Road Turns To Joy reminds us that we "walk not in order to arrive, but walk just for walking." Touching the earth with our feet is an opportunity to live in the here and now. Thich Nhat Hanh reminds us to enjoy each step and each breath in order to regain peace in difficult moments. The simple practice of walking with attention and mindfulness can bring the spirit of prayer into our everyday life. This book will appeal to anyone who would like to get more out of walking, from long-time meditators to those who are just looking for a way to make their walk around the block more meaningful.Features photographs of walking meditation from around the world.Foreword by Robert Aitken, author of Taking the Path of Zen

The Long Road Turns to Joy: A Guide to Walking Meditation

by Thich Nhat Hanh Robert Aitken

One of the few books focused completely on mindful walking and walking meditation. This revised edition of the best-selling title (nearly 80,000 copies sold to date) includes over 30 percent new material--including new walking meditation poems and practices--and provides a practical and inspirational introduction to this important practice. Written in Thich Nhat Hanh's clear and accessible style, Long Road Turns To Joy reminds us that we "walk not in order to arrive, but walk just for walking." Touching the earth with our feet is an opportunity to live in the here and now. Thich Nhat Hanh reminds us to enjoy each step and each breath in order to regain peace in difficult moments. The simple practice of walking with attention and mindfulness can bring the spirit of prayer into our everyday life. This book will appeal to anyone who would like to get more out of walking, from long-time meditators to those who are just looking for a way to make their walk around the block more meaningful.Features photographs of walking meditation from around the world.Foreword by Robert Aitken, author of Taking the Path of Zen

The Practice of Perfection

by Robert Aitken

Robert Aiken, author of Encouraging Words and Taking the Path of Zen, is America's most senior Zen Roshi. In this new book he presents the Ten Pãramitãs, of Transcendental Perfections--namely, giving, mortality, forbearance, zeal, focused meditation, wisdom, compassionate means, aspiration, spiritual power, and knowledge--two-thousand-year-old ideals that can serve us as both methods and goals. The Pãramitãs are the "skillful means" a person may employ to nurture and develop his or her spiritual and moral life. In religious instruction we are often met be restrictions, and are told what not to do. The Pãramitãs, explained from a Zen perspective, offer the seeker ten positive means of action, ten ways to live a life of clarity and grace in a modern world where neither seems easy or even possible. The transcendental perfections can lead us toward a life that is both spiritually invigorated and socially engaged. Aitken Roshi's way of teaching--anecdotal, careful, insightful, and easily accessible--leads us further along the path of harmony and balance. Each of the inspiring and instructional essays in this book is followed by a section in which Aitken answers questions most often asked by his own students in their course of study. The Practice of Perfection will be useful to seekers of all cultures and faiths.

The River of Heaven

by Robert Aitken

Known to many as the study of quiet stillness and introspection, Zen Buddhism distinguishes itself through brilliant flashes of insight and its terseness of expression. In River of Heaven these concepts and pillars lend themselves to an exploration of Haiku, one of the most delicate and interpretive poetic forms in the world. The haiku verse form, with its rigid structure and organic description is a superb means of studying Zen modes of thought because its seventeen syllables impose a limitation that confines the poet to vital experience. In Haiku as in Buddhism, the silences are as expressive as the words.In this volume, American Senior Zen Roshi Robert Aitken gives new insight into Haiku by poetic masters Basho, Issa, Buson, and Shiki. In presenting themes from Haiku and from Zen literature, Aitken illuminates the relationship between the two. Readers are certain to find this an invaluable and enjoyable experience for the remarkable revelation it offers.

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