Natural Killer Cells

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Synopsis
This volume contains a collection of research Natural Killer Cell methodologies relevant for both basic and translational research. These methodologies present new developments in the natural killer (NK) cell field, such as understanding the influence of NK cells metabolism on its function, identifying complexity of NK cell subsets through mass cytometry, and determining the emergence of memory NK cells in murine model of MCMV infection. Methods that study NK cells migration and cytotoxicity through endpoint analysis or live single cell imaging are also discussed. Chapters also describe methods pertaining to translational application of NK cells, such as "ex vivo" expansion of NK cells on K562 cell lines genetically modified to express either membrane bound IL-15 or membrane bound IL-21, large scale NK cell culture, current techniques for engineering NK cells to express chimeric antigen receptors of chemokine receptors using retroviral vectors, electroporation of mRNA, and the natural phenomenon of trogocytosis. Written in the highly successful "Methods in Molecular Biology "series format, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, "Natural Killer Cells: Methods and Protocols "is a valuable resource for researchers who not only want to understand mechanisms that govern NK cell behavior and diversity, but who also want to understand how to systematically evaluate NK cells for adoptive immunotherapy applications.
Copyright:
2016

Book Details

Book Quality:
Publisher Quality
ISBN-13:
9781493936847
Publisher:
Springer New York, New York, NY
Date of Addition:
Copyrighted By:
Springer
Adult content:
No
Language:
English
Has Image Descriptions:
No
Categories:
Nonfiction, Medicine
Submitted By:
Bookshare Staff
Usage Restrictions:
This is a copyrighted book.

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