Flavoprotein Protocols

By: Graeme A. Reid and Steven K. Chapman

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Synopsis: This collection of the most important methodologies for the analysis of the structure and function of flavoproteins ranges from wet to dry chemistry. These step-by-step methods-developed by leading experimentalists to work reliably-include the most commonly used techniques as well as a wide range of spectrophotometric protocols that allow direct monitoring of flavins in real time, in the steady state, or as reaction intermediates. Among the techniques detailed are freeze-quench methods, EPR spectroscopy, CD spectroscopy, vibrational spectroscopy (SERRS), and nuclear magnetic resonance. Also discussed are a variety of therapeutic applications for flavins and flavoproteins that go far beyond the use of riboflavin as a vitamin supplement. Flavoprotein Protocols offers to both experts in the field and to those working on a flavin-containing protein for the first time a wealth of proven methods and laboratory experience that will enhance their understanding of structure/function relationships in the proteins and of the effects of the protein environment on flavin chemistry.