Oligonucleotide Synthesis
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- Synopsis
- A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
- Copyright:
- 2005
Book Details
- Book Quality:
- Publisher Quality
- ISBN-13:
- 9781592598236
- Related ISBNs:
- 9781588292339
- Publisher:
- Humana Press, Totowa, NJ
- Date of Addition:
- 06/03/13
- Copyrighted By:
- Humana Press, Totowa, NJ
- Adult content:
- No
- Language:
- English
- Has Image Descriptions:
- No
- Categories:
- Nonfiction, Medicine
- Submitted By:
- Bookshare Staff
- Usage Restrictions:
- This is a copyrighted book.