Inositol Pyrophosphates: Methods and Protocols (Methods in Molecular Biology #2972)

By: Adolfo Saiardi and Dorothea Fiedler and Henning J. Jessen

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Synopsis: This detailed volume explores methods that reflect the explosion of interest in the important cellular messengers known as inositol pyrophosphates. After a brief historical perspective of the analytical approaches that have contributed to the development of this research field, the book collects methods that cover isolation, detection, and measurement of inositol pyrophosphates, followed by approaches to ascertain their mechanism of action, as well as a description of the use of experimental models that have been instrumental to many fundamental discoveries. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Inositol Pyrophosphates: Methods and Protocols serves as an ideal guide for researchers attempting to further our knowledge of this vibrant area of study.