- Table View
- List View
Enzyme Kinetics and Mechanism
by Paul F. Cook W. W. ClelandEnzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics. Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure. Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level. Enzymologists and molecular biologists will find the text a useful reference.
Enzyme Kinetics in Drug Metabolism
by Swati Nagar Upendra A. Argikar Donald J. TweedieDrug metabolism and transport are very important facets within the discipline of pharmaceutical sciences, with enzyme kinetic concepts utilized regularly in characterizing and modeling the disposition and elimination of drugs. Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications focuses on very practical aspects of applying kinetic principles to drug metabolizing enzymes and transporters. Divided into five convenient sections, topics include the fundamental principles of enzyme kinetics, the kinetics of oxidative and conjugative drug metabolizing enzymes and drug transporters, modeling approaches for both drug metabolizing enzymes and transporters including novel systems biology approaches, understanding of variability both experimental and interindividual (pharmacogenomic), and case studies that provide real life examples of applying these principles. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics especially suitable for the novice, in some cases step-by-step, readily reproducible protocols, and insights to help with troubleshooting and avoiding known pitfalls with extensive cross referencing to assist in learning. Authoritative and easily accessible, Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications serves as a very practical teaching tool for novice, non-mathematically trained scientists interested in these fundamental concepts and as an aid for their supervisors in teaching these principles.
Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications (Methods in Molecular Biology #2342)
by Swati Nagar Upendra A. Argikar Donald TweedieThis second edition further develops the principles of applying kinetic principles to drug metabolizing enzymes and transporters. Chapters are divided into six sections detailing fundamental principles of enzyme kinetics, enzyme and transporter structures, highlighting specific oxidative and conjugative drug metabolizing enzymes and drug transporters, modeling approaches for drug metabolizing enzymes and transporters, understanding of variability both experimental and interindividual (pharmacogenomic), and expanded case studies that provide real life examples of applying these principles. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, in some cases step-by-step instructions with readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls with extensive cross referencing to assist in learning. Authoritative and fully updated, Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications, Second Edition serves as a practical teaching tool for novice and advanced scientists interested in the fundamental concepts.
Enzyme- and Transporter-Based Drug-Drug Interactions
by A. David Rodrigues Raimund M. Peter K. Sandy PangGermination of the thought of "Enzymatic- and Transporter-Based Drug-Drug Interactions: Progress and Future Challenges" Proceedings came about as part of the annual meeting of The American Association of Pharmaceutical Scientists (AAPS) that was held in San Diego in November of 2007. The attendance of workshop by more than 250 pharmaceutical scientists reflected the increased interest in the area of drug-drug interactions (DDIs), the greater focus of PhRMA, academia, and regulatory agencies, and the rapid pace of growth in knowledge. One of the aims of the workshop was to address the progress made in quantitatively predicting enzyme- and transporter-based DDIs as well as highlighted areas where such predictions are poor or areas that remain challenging for the future. Because of the serious clinical implications, initiatives have arisen from the FDA (http://www.fda.gov/cber/gdlns/interactstud.htm) to highlight the importance of enzyme- and transporter-based DDIs. During the past ten to fifteen years, we have come to realize that transporters, in addition to enzymes, play a vital role in drug elimination. Such insight has been possible because of the continued growth in PK-ADME (pharmacokinetics-absorption-distribution-metabolism-excretion) knowledge, fueled by further advances in molecular biology, greater availability of human tissues, and the development of additional and sophisticated model systems and sensitive assay methods for studying drug metabolism and transport in vitro and in vivo. This has sparked an in-depth probing into mechanisms surrounding DDIs, resulting from ligand-induced changes in nuclear receptors, as well as alterations in transporter and enzyme expression and function. Despite such advances, the in vitro and in vivo study of drug interactions and the integration of various data sets remain challenging. Therefore, it has become apparent that a proceeding that serves to encapsulate current strategies, approaches, methods and applications is necessary. As Editors, we have assembled a number of opinion leaders and asked them to contribute chapters surrounding these issues. Many of these are the original Workshop speakers whereas others had been selected specially to contribute on topics related to basic and applied information that had not been covered in other reference texts on DDI. The resulting tome, entitled Enzyme- and Transporter-Based Drug Interactions: Progress and Future Challenges, comprises of four sections. Twenty-eight chapters covering various topics and perspectives related to the subject of metabolic and transporter-based drug-drug interactions are presented.
Enzyme-based Biosensors: Recent Advances and Applications in Healthcare
by Manashjit Gogoi Sanjukta Patra Debasree KunduThis book summarizes the principles, protocols, and applications of enzyme-based biosensors in healthcare. After providing a brief history and discussing the development of various biosensors used for medical applications, it explores the principles of enzyme-based biosensors and reviews a number of enzyme immobilization techniques. It then addresses the potential of enzyme-based biosensors in the diagnosis of infectious and non-infectious diseases; illustrates the potential of enzyme-based biosensors for non-invasive monitoring of biochemical biomarkers; and assesses the application of paper-based and microfluidic-based nanobiosensors in medical diagnostics. A one-stop resource on recent advances in enzyme-based biosensors in healthcare, this book will appeal to students, researchers and scientists in a range of disciplines, as well as entrepreneurs, and medical/healthcare professionals.
Enzymes and Their Inhibitors: Drug Development
by H. John Smith Claire SimonsFocusing on the development of enzyme inhibitors as therapeutic drugs, Enzymes and Their Inhibitors: Drug Development provides a concise overview of the chemistry of major types of enzymes and their inhibitors. The opening chapters introduce readers to the structure, functions, mechanisms, and kinetics of enzymes, including their use as disease mar
Enzymes in Farm Animal Nutrition
by John Patience Jason Lee Ralf Greiner Kyle Brown Ceinwen Evans Hamish Irving Jari Vehmanperä Kari Juntunen Qingyun Li Amy Petry Aaron Cowieson Daniel Menezes-Blackburn Ursula KonietznyFrom alpha-galactosidases to xylanases, Enzymes in Farm Animal Nutrition provides a comprehensive guide to all aspects associated with enzyme-supplemented animal feeds. It details the history and size of the feed enzyme market, before describing how feed enzymes are manufactured and employed in monogastric, aqua and ruminant diets. This new edition explores considerable advances such as the use of enzymes in fish and shrimp diets, new understanding of how phytases function in the animal, NSPase research and enzymes' extended use in ruminant markets. This book also: - Provides comprehensive coverage of all topics relating to the production, use, co-operativity and analysis of feed enzymes. - Is fully updated throughout, revealing significant developments such as new methods to deliver enzymes (formulations, encapsulations, and liquid spray systems) and advances in enzyme analysis. - Includes brand new chapters on combinations of enzymes, antibiotic-free diets and how to measure response in feed-enzyme trials. Covering biochemistry, enzymology and characteristics relevant to animal feed use, this book forms a valuable resource for academics and students of animal nutrition and production, as well as professionals in the animal feed industry.
Enzymes in Food Technology: Improvements and Innovations
by Mohammed KuddusThe integration of enzymes in food processing is well known, and dedicated research is continually being pursued to address the global food crisis. This book provides a broad, up-to-date overview of the enzymes used in food technology. It discusses microbial, plant and animal enzymes in the context of their applications in the food sector; process of immobilization; thermal and operational stability; increased product specificity and specific activity; enzyme engineering; implementation of high-throughput techniques; screening of relatively unexplored environments; and development of more efficient enzymes. Offering a comprehensive reference resource on the most progressive field of food technology, this book is of interest to professionals, scientists and academics in the food and biotech industries.
Enzymes of Nucleic Acid Synthesis and Modification: Volume 1: DNA Enzymes
by Samson T. JacobThe First volume gives an overview of the enzymes involved in DNA synthesis and modification; the second volume deals with the RNA-enzymes. Although the major emphasis of the book is on eukaryotic enzymes, a separate chapter dealing with prokaryotic DNA repair enzymes has been included to discuss the major advances in this field in recent years. There are two separate chapters on RNA polymerases to provide a comprehensive coverage of the enzymes from lower eukaryotes, plants and higher eukaryotes.
Enzymes of Nucleic Acid Synthesis and Modification: Volume 2: RNA Enzymes
by Samson T. JacobThe First volume gives an overview of the enzymes involved in DNA synthesis and modification; the second volume deals with the RNA-enzymes. Although the major emphasis of the book is on eukaryotic enzymes, a separate chapter dealing with prokaryotic DNA repair enzymes has been included to discuss the major advances in this field in recent years. There are two separate chapters on RNA polymerases to provide a comprehensive coverage of the enzymes from lower eukaryotes, plants and higher eukaryotes.
Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis
by Robert A. CopelandENZYMES A complete and approachable introduction to the study of enzymes, from theory to practice Enzymes catalyze the bulk of important biological processes, both metabolic and biochemical. They are specialized proteins whose function is determined by their structure, understanding which is therefore a key focus of biological, pharmacological, and agrarian research, among many others. A thorough knowledge of enzyme structure, pathways, and mechanisms is a fundamental building block of the life sciences and all others connected to them. Enzymes offers a detailed introduction to this critical subject. It analyzes enzyme proteins at the structural level and details the mechanisms by which they perform their catalyzing functions. The book’s in-depth engagement with primary literature and up-to-date research allows it to continuously deploy illustrative examples and connect readers with further research on key subjects. Fully updated after decades as the standard text, this book unlocks a thriving field of biological and biochemical research. Readers of the third edition of Enzymes will also find: Expanded chapters on steady-state and transient-state enzyme kinetics, structural components of enzymes, and more New chapters on enzyme regulation, enzyme-macromolecule interactions, enzyme evolution, and enzymes in human health Key Learning Points at the beginning of each chapter to assist students and instructors Enzymes promises to continue as the standard reference on this subject for practitioners of the life sciences and related fields in both academia and industry.
Enzymology of Complex Alpha-Glucans
by Felix NitschkeGlycogen and Starch: So Similar, yet so Different. Both carbohydrates are central to the primary metabolism of a large part of the living kingdom. Generally, animals, fungi, and bacteria store glycogen, while plants largely rely on starch. This book provides a broad and current view on both glycogen and starch, in lower and higher organisms. Beside biochemistry, physiology and regulation of glycogen and starch metabolism, the reader can expect an insight into glycogen storage diseases, select methods and relevant techniques. While significant progress has been made in both fields, this volume emphasizes an opportunity of collaboration for researchers working on a major intersection of the living world.
Eosinophilic Esophagitis
by Jonathan E. Markowitz Chris A. LiacourasThis volume provides a history of Eosinophilic Esophagitis (EoE), a basic understanding of the physiology of the eosinophil, and a current understanding of the pathophysiology and genetics of EoE. The emphasis is on clinical applications including presenting symptoms, diagnosis and treatment options for patients with EoE. Written by both pediatric and adult experts in the fields of gastroenterology, allergy and pathology, this volume includes the most up to date information. Providing practical information useful in the treatment of patients, this book will be of great value to gastroenterologists, allergists, pathologists, medical residents, fellows, internists, and general practitioners who treat patients with eosinophilic esophagitis.
Eosinophils
by Garry M. WalshThe eosinophil is an enigmatic cell with a continuing ability to fascinate. In this book, experts in the field of eosinophil biology comprehensively update our knowledge on the human eosinophil in health and disease.
Eosinophils in Allergy and Inflammation (Clinical Allergy And Immunology Ser. #2)
by Gerald J. GleichThis work presents the proceedings of a conference held at Adare Manor, County Limerick, Ireland. It provides an updated, in-depth review of the biological role of eosinophils in allergic diseases, summarizing basic knowledge of these unique cationic proteins. The book features an annotated discussion of the conference's post-presentation question-and-answer session.
Eosinophils: Methods and Protocols (Methods in Molecular Biology #2241)
by Garry M. WalshThis second edition of Eosinophils: Methods and Protocols updates several techniques from the first edition together with novel in vitro and in vivo-based methodologies. Written by internationally recognized expert authorities, this volume provides vital techniques from eosinophil purification to experimental modelling, with each technique spelled out in clear and straightforward terms, assuming no previous knowledge of the method and without necessitating sourcing additional information elsewhere. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, with tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Eosinophils: Methods and Protocols, Second Edition provides the practical means to extend our knowledge of eosinophil function in health and disease, underpinning research that may in turn lead to new hypotheses for future examinations into the role of this intriguing and enigmatic leukocyte.
Epic Measures: One Doctor. Seven Billion Patients.
by Jeremy N. SmithMoneyball meets medicine in this remarkable chronicle of one of the greatest scientific quests of our time and the visionary mastermind behind it.Medical doctor and economist Christopher Murray began the Global Burden of Disease study to gain a truer understanding of how we live and how we die. While it is one of the largest scientific projects ever attempted—as breathtaking as the first moon landing or the Human Genome Project—the questions it answers are meaningful for every one of us: What are the world's health problems? Who do they hurt? How much? Where? Why?Murray argues that the ideal existence isn't simply the longest, but the one lived well and with the least illness. Until we can accurately measure global health issues, we cannot understand what makes us sick or do much to improve it. Challenging the accepted wisdom of the WHO and the UN, the charismatic and controversial health maverick has made enemies—as well as some influential friends, including Bill Gates who gave Murray a $100 million grant.Told with novelistic verve by acclaimed journalist Jeremy N. Smith, the story of Murray's lifelong determination to understand how we live and die encompasses wars and famines, presidents and activists, billionaires and billions of people worldwide living in poverty. It shows the human side of scientific revolutions and of revolutionary scientists—their breakthroughs and setbacks, their genius and their flaws, their champions and their critics—as they strive to bring the news of their findings to the world. This transformational effort is far from over, but the story of its genesis and impact is already an epic tale.
Epicardial Adipose Tissue: From Cell to Clinic (Contemporary Cardiology)
by Gianluca IacobellisThis book is the first authoritative and comprehensive volume dedicated to epicardial adipose tissue (EAT). It provides an up-to-date and highly illustrated synopsis of the anatomical, biomolecular, genetic, imaging features, and clinical applications of EAT and its role in cardiovascular disease.It relays to the reader a contemporary view of the emerging interplay between the heart and adiposity-related diseases. In addition, this volume discusses the clinical implications and therapeutic targets of EAT in atrial fibrillation, heart failure and coronary artery disease.Comprehensive yet focused, Epicardial Adipose Tissue: From Cell to Clinic is an essential resource for physicians, residents, fellows, and medical students in cardiology, endocrinology, primary care, and health promotion and disease prevention.
Epidemic Analytics for Decision Supports in COVID19 Crisis
by Simon James Fong Joao Alexandre Lobo MarquesCovid-19 has hit the world unprepared, as the deadliest pandemic of the century. Governments and authorities, as leaders and decision makers fighting against the virus, enormously tap on the power of AI and its data analytics models for urgent decision supports at the greatest efforts, ever seen from human history. This book showcases a collection of important data analytics models that were used during the epidemic, and discusses and compares their efficacy and limitations. Readers who from both healthcare industries and academia can gain unique insights on how data analytics models were designed and applied on epidemic data. Taking Covid-19 as a case study, readers especially those who are working in similar fields, would be better prepared in case a new wave of virus epidemic may arise again in the near future.
Epidemic City: The Politics of Public Health in New York
by James ColgroveAn insightful chronicle of the changing public health demands in New York City. The first permanent Board of Health in the United States was created in response to a cholera outbreak in New York City in 1866. By the mid-twentieth century, thanks to landmark achievements in vaccinations, medical data collection, and community health, the NYC Department of Health had become the nation’s gold standard for public health. However, as the city’s population grew in number and diversity, the department struggled to balance its efforts between the treatment of diseases—such as AIDS, tuberculosis, and West Nile Virus—and the prevention of illness-causing factors like lead paint, heroin addiction, homelessness, smoking, and unhealthy foods. In Epidemic City, historian of public health James Colgrove chronicles the challenges faced by the health department since New York City’s mid-twentieth-century “peak” in public health provision. This insightful volume draws on archival research and oral histories to examine how the provision of public health has adapted to the competing demands of diverse public needs, public perceptions, and political pressure. Epidemic City analyzes the perspectives and efforts of the people responsible for the city’s public health from the 1960s to the present—a time that brought new challenges, such as budget and staffing shortages, and new threats like bioterrorism. Faced with controversies such as needle exchange programs and AIDS reporting, the health department struggled to maintain a delicate balance between its primary focus on illness prevention and the need to ensure public and political support for its activities. In the past decade, after the 9/11 attacks and bioterrorism scares partially diverted public health efforts from illness prevention to threat response, Mayor Michael Bloomberg and Health Commissioner Thomas Frieden were still able to pass New York’s Clean Indoor Air Act restricting smoking and significant regulations on trans-fats used by restaurants. This legislation—preventative in nature much like the department’s original sanitary code—reflects a return to the nineteenth century roots of public health, when public health measures were often overtly paternalistic. The assertive laws conceived by Frieden and executed by Bloomberg demonstrate how far the mandate of public health can extend when backed by committed government officials. Epidemic City provides a compelling historical analysis of the individuals and groups tasked with negotiating the fine line between public health and political considerations. By examining the department’s successes and failures during the ambitious social programs of the 1960s, the fiscal crisis of the 1970s, the struggles with poverty and homelessness in the 1980s and 1990s, and in the post-9/11 era, Epidemic City shows how the NYC Department of Health has defined the role and scope of public health services for the entire nation.
Epidemic Illusions: On the Coloniality of Global Public Health
by Eugene T RichardsonA physician-anthropologist explores how public health practices--from epidemiological modeling to outbreak containment--help perpetuate global inequities.In Epidemic Illusions, Eugene Richardson, a physician and an anthropologist, contends that public health practices--from epidemiological modeling and outbreak containment to Big Data and causal inference--play an essential role in perpetuating a range of global inequities. Drawing on postcolonial theory, medical anthropology, and critical science studies, Richardson demonstrates the ways in which the flagship discipline of epidemiology has been shaped by the colonial, racist, and patriarchal system that had its inception in 1492.
Epidemic Orientalism: Race, Capital, and the Governance of Infectious Disease
by Alexandre I. WhiteFor many residents of Western nations, COVID-19 was the first time they experienced the effects of an uncontrolled epidemic. This is in part due to a series of little-known regulations that have aimed to protect the global north from epidemic threats for the last two centuries, starting with International Sanitary Conferences in 1851 and culminating in the present with the International Health Regulations, which organize epidemic responses through the World Health Organization. Unlike other equity-focused global health initiatives, their mission—to establish "the maximum protections from infectious disease with the minimum effect on trade and traffic"—has remained the same since their founding. Using this as his starting point, Alexandre White reveals the Western capitalist interests, racism and xenophobia, and political power plays underpinning the regulatory efforts that came out of the project to manage the international spread of infectious disease. He examines how these regulations are formatted; how their framers conceive of epidemic spread; and the types of bodies and spaces it is suggested that these regulations map onto. Proposing a modified reinterpretation of Edward Said's concept of orientalism, White invites us to consider "epidemic orientalism" as a framework within which to explore the imperial and colonial roots of modern epidemic disease control.
Epidemic Risk Analysis and Assessment in Transport Services: COVID-19 and Other Viruses
by Rafał Burdzik"Accurate and fully explicit mathematical models and derivations make the proposed method truly universal irrespective of the geographical location and the kind of virus epidemic." Minvydas Ragulskis, Kaunas University of Technology, Lithuania The effects of a pandemic on public, personal and freight transport can be sudden and massive, and yet transport is vital to the functioning of an advanced economy and society. On the other hand, transport, due to social mobility, has a decisive influence on the speed and scope of epidemic spread. This book presents a complete methodology for assessing the hazards, and probability and risks of viral transmission on transport services, using as a detailed example the SARS-CoV-2 coronavirus pandemic. It gives proposals and recommendations for estimating human deaths caused by virus infection in transport. Significantly, it considers not only passenger transport but also freight transport, such as delivery or parcel services. The tools include a matrix of hazard assessment in various transportation services, with a methodology for estimating the probability of virus transmission through both droplets and surface contact. These allow estimation of the effects of infections and consequent epidemic risk in all kinds of transport services, including freight, and provide methods for forecasting and risk management which determine transport safety. Rafał Burdzik is a professor in the Faculty of Transport and Aviation Engineering at Silesian University of Technology, Poland, with more than 20 years of transport research experience.
Epidemic Risk Reduction: A Civil Protection Approach
by Pawel GromekEpidemic Risk Reduction: A Civil Protection Approach focuses on disaster risk reduction (DRR) relative to epidemic outbreak and the concept of civil protection and public safety. First and foremost, the primary role of public officials and agencies—including police, military, public health, and emergency management and response professionals—must be the safety and security the citizens. The book provides practical solutions and policies that can be enacted and instituted to protect human life and health as well as property and environment in the scope of human survival, providing essential services, and providing for peoples’ basic needs. The author examines epidemics as disasters, similar to natural hazards or other large-scale events that require planning, preparedness, mitigation, and response. The approach is unique in looking at tools for risk assessment and strategic planning formulated around civil protection measures as a means to reduce and mitigate risk. Often, preparedness and response planning is siloed with an epidemiological and medical response at the forefront. While absolutely important and essential, doing so—in conjunction with coordinated government efforts to provide necessary supplies, security, and logistics—is equally critical to meet the public’s needs and provide a coordinated response. Key Features: Examines the phenomenon of epidemics and hazards, explaining that they are often multi-hazard Provides public leaders with various considerations to meet the challenge of managing the complexity of various threat vectors Details universal terms and definitions—e.g., disaster, risk, and epidemic—using diagrams, illustrations, and analogies to clearly explain critical concepts Presents epidemic risk reduction strategies—based on the lead role that disaster, emergency management, and state/civil authorities need to play—focusing primarily on individual health and security Epidemic Risk Reduction: A Civil Protection Approach establishes a common language which will help in overcoming barriers for more coordinated efforts focused on epidemic management and operational response. While drawing on current research, and based on theoretical knowledge, the book also provides practical examples from around the world that look at real-world response efforts. This includes recommendations on how politicians, crisis response personnel, emergency planners, and public administrators and health professionals can better plan and prepare for future epidemics.
Epidemic of Care: A Call for Safer, Better, and More Accountable Health Care
by George C. Halvorson George J. IshamRecognizing a crisis in American health care, but rejecting any solutions that are not "market-based," Halvorson (chair and CEO, Kaiser Permanente) and Isham (chief health officer, HealthPartners) explore some of the reasons for rising health care costs (absolving the HMOs for any responsibility) and make suggestions for improving the situation. Their proposed system rests largely on promoting evidence- based care for physicians and transferring greater costs to patients to improve "efficiency."