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Epistasis: Methods and Protocols (Methods in Molecular Biology #2212)

This volume explores methods and protocols for detecting epistasis from genetic data. Chapters provide methods and protocols demonstrating approaches to identify epistasis, genetic epistasis testing, genome-wide epistatic SNP networks, epistasis detection through machine learning, and complex interaction analysis using trigenic synthetic genetic array (τ-SGA). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Epistasis: Methods and Protocols aims to ensure successful results in the further study of this vital field. "Simulating Evolution in Asexual Populations with Epistasis” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Epistemic Justice in Mental Healthcare: Recognising Agency and Promoting Virtues Across the Life Span

This open access book explores epistemic justice in mental healthcare, bringing together perspectives from psychologists, psychiatrists, philosophers, activists, and lived experience researchers. Through eight chapters, authors identify threats to the agency of people who hear voices, experience depression, have psychotic symptoms, live with dementia, are diagnosed with personality disorders, and face serious mental health issues while receiving palliative care. Considering the power asymmetries in clinical interactions, where patients are vulnerable and healthcare professionals are uniquely placed to offer support, this book reaffirms the importance of recognizing patients as agents and collaborators. Topics covered include trust in the therapeutic relationship, dignity at the end of life, the social dimension of health, stigma in an acute ward, the harm caused by biases and stereotypes, the role of clinical communication, and the promise of digital health. Students, academic researchers, practitioners, as well as mental health charities will benefit from this timely collection.

Epithelial Cell Culture Protocols

Well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. The emphasis is on the analysis and assessment of epithelial cells, for example by looking at apoptosis and integrins, or by measuring membrane capacitance and confluence. Also described in step-by-step detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria. Epithelial Cell Culture Protocols offers a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells.

Epithelial Morphogenesis in Development and Disease

Bringing together a series of articles on the structural, functional, and developmental characteristics of epithelia, this volume represents a timely and valuable contribution to a growing field of study.

Epitope Mapping Protocols

Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular BiologyTM series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping.

Epitope Mapping Protocols (Methods in Molecular Biology #2937)

This detailed new edition explores methodologies for the characterization of antibody-antigen interactions tailored to different applications and requirements. Beginning with an overview of key concepts and definitions related to antibody epitopes, the book continues with sections on structural biology methods, display technologies to analyze libraries of proteins or protein arrays, techniques based on protein labeling, as well as computational approaches and binding assays that rely on binding interactions or competition. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Epitope Mapping Protocols, Fourth Edition serves as an ideal resource for scientists from a wide range of fields involved in critical antibody research. Chapter 19 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Epitranscriptomics: Methods and Protocols (Methods in Molecular Biology #1870)

This volume provides readers with the latest technologies to study changes in the epitranscriptome. The protocols described in this book explore both targeted and unbiased high-throughput analysis associated with post-transcriptional RNA modification. The chapters in this book also cover specific topics such as transcriptome-wide detection of 5-methylcytosine; HAMR; iRNA-2OM; genome-wide annotation of circRNAs; immune-northern blotting; and detection and quantification of pseudouridine in RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and comprehensive, Epitranscriptomics: Methods and Protocols is an important resource for both expert and novice scientists who are interested in learning more about this field.

Eponyms and Names in Obstetrics and Gynaecology: Eponyms And Names In Obstetrics And Gynaecology

Few specialties have a longer or richer eponymous background than obstetrics and gynaecology. Eponyms add a human side to an increasingly technical profession and represent the historic tradition and language of the speciality. This collection aims to perpetuate the names and contributions of pioneers and offer introductory profiles to the founders in whose steps we follow. This third edition includes 26 new entries, as well as expanded detail, illustration and quotation for existing entries. Biographical data and historical and medical context are discussed for each of the 391 names, with reference to 34 countries, reflecting the field's far reaching origins. More than 1700 original references feature, alongside an extensive bibliography of more than 2500 linked references to assist readers searching for more detailed information. This is a volume for physicians, midwives, medical historians, medical ethicists and all those interested in the history and evolution of obstetrical and gynaecological treatment.

Eponyms in Surgery and Anatomy of the Liver, Bile Ducts and Pancreas

For surgeons, physicians, and anatomists involved in the management and study of disorders of the liver, bile ducts and pancreas, eponyms are part of everyday communication. They help to describe anatomical features, operative procedures, surgical instruments, and diseases. Unfortunately, many have become distorted or are inaccurately applied. Few of us understand their derivation or the remarkable people and controversies behind them.This book explores the origins of seventy eponyms in the field of hepatobiliary and pancreatic surgery and anatomy. Each section is deliberately short and intended for quick reference, providing accurate information about the origin of the eponym and the figure behind it.Meticulously researched, and beautifully illustrated with more than 150 photographs, Eponyms in Surgery and Anatomy of the Liver, Bile Ducts and Pancreas is aimed at surgeons, physicians and anatomists, and is sure to enrich the reader's historical perspective of this fascinating branch of surgery and anatomy.

Epstein Barr Virus

This volume explores data from the applications of molecular biological methods and the applications of recent immunological and cytogenetic methods in Epstein-Barr Virus (EBV) that will offer readers possible new solutions to the unresolved problems in the EBV field. Chapters in this book cover topics such as: viral life cycle, latency, EBV-associated diseases and EBV diagnostics; in vitro methods including organotypic cultures for the analysis of EBV-epithelial cell interactions; identification of the interacting viral and cellular proteins using affinity purification-mass spectrometry methods; 3D telomere FISH; transcription analysis using high-throughput RNA sequencing, qPCR and nuclear run-on assay; analysis of viral and cellular microRNAs; isolation and characterization of exosomes and the assessment of their function; characterization of the viral genome by terminal repeat analysis and sequencing; the use of chromatin immunoprecipitation coupled sequencing (ChIP-Seq) for the analysis of Zta-DNA interactions; epigenetic analysis by bisulfite sequencing and ChIP; novel in vivo models for the study of EBV infection; and how immunological, virological, tissue culture and molecular methods can be combined to yield Good Manufacturing Practice-compliant EBV-specific T cells for the immunotherapy of EBV-associated post-transplant lymphoproliferative diseases (PTLD). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Epstein Barr-Virus: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.

Epstein Barr Virus Volume 2

Epstein Barr virus (EBV) was discovered as the first human tumor virus around 50 years ago. Since its discovery in Burkitt's lymphoma it has been associated with various other malignancies, infectious mononucleosis and even autoimmune diseases. The two book volumes on EBV summarize the first 50 years of research on this tumor virus, starting with historical perspectives on discovery, oncogenicity and immune control, reviewing the role that the virus plays in the various associated diseases and concluding with a discussion on how the immune system keeps persistent EBV infection under control in healthy EBV carriers and can be used to treat EBV associated diseases. The respective 32 chapters are written by international experts from three continents for health care providers, biomedical researchers and patients that are affected by EBV. The assembled knowledge should help to understand EBV associated diseases better and to develop EBV specific vaccination in the near future.

Epstein-Barr Virus

Filling a gap in the literature, this guide analyzes EBV infection and all of its associated disorders including infectious mononucleosis, Burkitt lymphoma, and Hodgkin's disease. Opening with a historical introduction, the reference progresses from molecular virology, epidemiology, immunology, and pathology to clinical presentation, diagnosis, dis

Epstein-Barr Virus Protocols

Joanna Wilson and Gerhard May have assembled a collection of the key molecular biology protocols used in the analysis of Epstein-Barr virus (EBV), along with a series of valuable immunology, cell biology, and transgenic mouse protocols. These readily reproducible techniques include methods for gene expression with mini-EBV plasmids, for expression analysis by FISH, for EBV detection and quantitation, and for cell proliferation and death assays. In addition, there are EBV-based vectors, an up-to-date map of EBV, a comprehensive table of available latent protein antisera, and assays from in vitro to cell to organ to organism levels. Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating the life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures for EBV associated diseases, including several forms of human cancer.

Equal Care: Health Equity, Social Democracy, And The Egalitarian State

Equal Opportunity and the Case for State Sponsored Ectogenesis

Ectogenesis refers to the artificial gestation of a fetus outside the womb. Despite certain advantages for women's reproductive liberty, feminist groups remain divided regarding this technology. This book argues that reproduction imposes unjust burdens on women, and thus the ideals of equal opportunity demand continued research into ectogenesis.

Equalising Opportunities, Minimising Oppression: A Critical Review of Anti-Discriminatory Policies in Health and Social Welfare

Anti-Racist Practice (ARP), Anti-Discriminatory Practice (ADP) and Anti-Oppressive Practice (AOP) form a trinity of concepts, nested into one another, which have evolved in welfare services over the last fifteen years. They tend to have developed as forms of practice panaceas and as a result have been subject to both unrealistic expectations and, at times, to political ridicule. This book clarifies the distinctions between three key concepts - ARP, ADP and AOP. Critically and constructively analysing these three approaches to practice it reappraises their potential in the light of emerging equality issues in the health service With contributions from leading teachers and practitioners in the field, Equalising Opportunities provides students and practitioners in health and social care with a clear overview of an area where there is much confusion and imperfect understanding.

Equality within Our Lifetimes: How Laws and Policies Can Close—or Widen—Gender Gaps in Economies Worldwide

A free ebook version of this title is available through Luminos, University of California Press’s Open Access publishing program. Visit www.luminosoa.org to learn more. Well into the twenty-first century, achieving gender equality in the economy remains unfinished business. Worldwide, women’s employment, income, and leadership opportunities lag men’s. Building and using a one-of-a-kind database that covers 193 countries, this book systematically analyzes how far we’ve come and how far we have to go in adopting evidence-based solutions to close the gaps. Spanning topics including girls’ education, employment discrimination of all kinds, sexual harassment, and caregiving needs across the life course, the authors bring the findings to life through global maps, stories of laws’ impact in courts and beyond, and case studies of making change. A powerful call to action, Equality within Our Lifetimes reveals how gender equality is both feasible and urgently needed to address some of the greatest challenges of our generation.

Equine Anesthesia and Co-Existing Disease

Equine Anesthesia and Co-Existing Disease offers practical guidance on anesthetizing horses with pre-existing diseases or other unique conditions. Filling a significant gap in available literature, this authoritative reference is the ideal companion to existing publications on basic management principles, equipment, and complications in equine anesthesia. Detailed chapters, co-authored by anesthesiologists and other allied specialists, offer a body-system approach to anesthesia considerations in horses presenting with a variety of disease conditions. Each chapter contains foundational knowledge such as pathophysiology or diagnostic techniques, clinical images, practical information for pharmacologic selection, and technical requirements for completion of procedures. The text covers equine anesthesia management relevant to respiratory, neuromuscular, and gastrointestinal diseases, cardiac and orthopedic procedures, diagnostic imaging and unique therapies, and more. Designed to allow quick and easy reference to vital information, this valuable clinical resource: Provides complete coverage of anesthetic management of horses with existing conditions or diseases Describes how pre-existing conditions can affect anesthesia in equine patients Includes coverage of accident and error management, induction, and recovery Discusses how anesthesia management differs for other species such as domesticated donkeys and mules Equine Anesthesia and Co-Existing Disease is a must-have reference and clinical guide for everyone involved in the anesthetization of equine patients, including equine general practitioners, anesthesiologists, specialists in related areas, residents in veterinary anesthesiology, and equine anesthesia technicians.

Equine Anesthesia and Pain Management: A Color Handbook (Veterinary Color Handbook Series)

Equine Anesthesia and Pain Management: A Color Handbook brings together key information for clinicians in an easy-to-use, problem-orientated format. It disseminates a wealth of knowledge about horse, donkey and mule anesthesia and pain management in a quick reference style, with a focus on clinical practice. Fifteen chapters by expert contributors cover everything from anesthetic equipment, premedication and physical restraint, to total intravenous anesthesia, inhalant anesthesia and partial intravenous anesthesia, recovery, complications and euthanasia. Over 250 superb color photographs and diagrams bring the material to life. This book will be invaluable to all those who need practical information easily to hand, whether equine practitioner, veterinary technician or nurse, or veterinary student.

Equine Bandaging, Splinting, and Casting Techniques

Equine Bandaging, Splinting, and Casting Techniques A practical reference manual dealing with a vital component of clinical practice in equine medicine The application of bandages, splints, and casts is an essential part of equine surgical and veterinary care. Traditionally, however, there have been few available resources wholly dedicated to application techniques. The result is that equine veterinary practitioners learn to bandage, splint, and cast on the job, with highly variable results; some practitioners are unwilling even to attempt a cast, spurning this valuable healing tool entirely rather than attempt it from an uncertain base of knowledge. Equine Bandaging, Splinting, and Casting Techniques offers the first comprehensive reference to this specific set of techniques and their applications in equine veterinary medicine. It promises to cultivate a rigorous, clinically tested, consistent standard of care that will improve patient outcomes and long-term owner costs. It is a must-own for any veterinary practitioner who works with equine patients. Equine Bandaging, Splinting, and Casting Techniques readers will also find: Step-by-step organization guides reader smoothly through each process Practical tips and advice for improving quality and appearance of bandages and casts Over 270 color images, including clinical examples and radiographs, supplementing every listed technique Equine Bandaging, Splinting, and Casting Techniques is ideal for all veterinary practitioners, technicians, and students interested in equine care.

Equine Behaviour in Practice: Understanding Horses and Treating Behaviour Problems

Combining an in-depth exploration of equine behaviour with clinical practice from a new progressive perspective, Equine Behaviour in Practice: Understanding Horses and Treating Behaviour Problems focuses not just on the horse’s behaviour but also on the behaviour of the human interacting with the horse. Acclaimed Clinical Equine Behaviourist Debbie Busby delivers evidence-based knowledge that will equip readers with the understanding and skills to become effective equine behaviour consultants. Supported by considered discussion of how and why horses sometimes struggle to cope, the book comprehensively explains how to consult on and treat a range of equine behaviour problems. Rather than providing a formulaic "here’s the problem; here’s how to fix it", Busby encourages readers to develop a critical biopsychosocial evaluation based on an understanding of how horses evolved, their ethological and physiological adaptations, how they learn, and how their welfare and wellbeing can be assessed. KEY FEATURES: Part 1 explains theories of equine evolution and the thin veneer of domestication Part 2 describes psychological and physiological theories of how horses learn, and how this can be applied in training and behaviour modification Part 3 illustrates this with reference to relevant legislation and widely used animal welfare models, as well as assessments of abnormal and stereotypic behaviour and practical aspects of measuring welfare Part 4 turns to the theory and practice of behaviour consulting, in three chapters divided into elements of behavioural assessment and treatment planning Part 5 examines the use of psychopharmaceuticals in behaviour therapy, discussing the roles of the behaviour consultant and the referring veterinary surgeon Bridging the gap between science and practice, this book is a must-read for equine behaviour and veterinary students, equine behaviour consultants and practitioners, horse trainers, interested leisure riders and competition riders. It will empower equine professionals to combine behavioural and welfare knowledge with counselling and coaching skills to support horse owners in a mutual flourishing, enriching the lives of both species.

Equine Behaviour: Principles and Practice

Understanding the flexibility and limits of behaviour is essential to improving both the horse's welfare and its performance. This book tackles the fundamental principles which will enable owners, riders, trainers and students to understand scientific principles and apply them in practice. Subjects covered include the analysis of influences on equine behaviour, the perceptual world of the horse, learning and training techniques including the latest developments in "join-up" and "imprint training".

Equine Clinical Immunology

Equine Clinical Immunology offers comprehensive information on equine immunological disorders.* Provides a complete, equine-specific reference on clinical immunology * Focuses on clinically relevant information for the diagnosis and treatment of horses with immune disorders * Illustrates the concepts discussed using drawings, photographs, and tables * Presents key concepts, clinical assessment information, and treatment approaches in text boxes for ease of use * Offers a practical, clinically oriented approach ideal for equine specialists

Equine Clinical Medicine, Surgery and Reproduction, Second Edition (Manson Ser.)

This fully-revised new edition of the bestselling Equine Clinical Medicine, Surgery and Reproduction is supported by over 1800 illustrations of the highest quality: colour photographs, radiographic and ultrasound images, and diagrams. System-based, the chapters introduce each individual system with precise information on the relevant basic anatomy and physiology, standard clinical examination techniques and useful differential diagnostic aids. This is followed by diseases and disorders that are pertinent to that system, grouped together either anatomically or based on presenting clinical signs. Each condition is described using consistent headings: definition/overview, etiology and pathophysiology, clinical presentation, diagnosis, differential diagnoses, management/treatment, and prognosis. Additional chapters deal with the foal, with wounds and behavioural problems. New to the second edition is expanded material on the musculoskeletal system, a new muscle section, updated information on reproduction, including assisted breeding and laparoscopic techniques, more information on diagnostic tests including over ground endoscopy, chest ultrasonography and head CT, and a significantly expanded surgical section. The material on equine dentistry, neurology, endocrine system and the liver has also been considerably developed. The focus throughout remains on providing clinically relevant information required for practical case management, plus sufficient background on causes and disease processes to enable readers to understand the conditions and the rationale for diagnostic and treatment options. Expert authors come together under the editorship of Dr Graham Munroe to create a textbook that will be of lasting value as a teaching and training resource for equine specialists and students in veterinary medicine and related equine courses, as well as a ready reference for non-specialist practitioners.

Equine Clinical Nutrition

EQUINE CLINICAL NUTRITION Authoritative resource on the nutritional management of horses, now incorporating the iterative learning process The second edition of Equine Clinical Nutrition is a fully updated and expanded revision of the classic student text on nutritional management of horses, covering updated nutrient recommendations, rations, feeding management, clinical nutrition and many other important topics in the field. To aid in reader comprehension, this new edition takes a new instructional approach to nutritional management using an iterative sequence of defined procedures. Divided into distinct sections for easy accessibility, this book is a comprehensive resource for feeding practices and management of healthy and sick horses alike. A thorough understanding of life stages, anatomy, physiology, and behavior underpins the practice of clinical nutrition. Sample topics covered in Equine Clinical Nutrition include: The evolution of horses to changing food supply, the importance of their microbiome, and the behavior patterns of feeding and drinking Nutrient metabolism of water, energy, protein, minerals, and vitamins, plus ration assessment, farm investigations, forages, and toxic plants Manufactured feeds, dietary supplements, USA feed regulations, and feed safety protocols Nutritional assessment of horses by life stage, recognizing pain and discomfort behaviors, and dietary management of weight and major system disorders Equine Clinical Nutrition is an essential text for students of veterinary medicine, animal science, pre-veterinary programs, and a desk reference for equine practitioners wanting practical clinical feeding recommendations. With comprehensive coverage of the topic, it is an essential text for everything related to nutrition in horses.