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In Silico Technologies in Drug Target Identification and Validation (Drug Discovery Series)
by Scott Markel Darryl LeónThe pharmaceutical industry relies on numerous well-designed experiments involving high-throughput techniques and in silico approaches to analyze potential drug targets. These in silico methods are often predictive, yielding faster and less expensive analyses than traditional in vivo or in vitro procedures. In Silico Technologies in Drug Target Ide
In Silico Tools for Gene Discovery
by Marcus Hinchcliffe Bing YuAs functional genomics has become one of the major focuses in molecular biology, the need for more sophisticated tools to assist in the identification of the functionality of undefined genes and the correlation of DNA variants with a particular phenotype has increased greatly. In Silico Tools for Gene Discovery collects many common and useful in silico tools available today. The volume begins by investigating locus mapping information on linkage analysis, association mapping, integrative analysis, and exome analysis as well as tools for DNA marker selection, in silico PCR, and statistical analysis. It continues with a section on gene discovery from a defined locus, including gene prioritization, knowledge tracking, and data mining, and concludes with several useful in silico tools presented for the functional characterization of genes, which include DNA sequencing analysis, variant characterization, as well as RNA and protein analysis. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, step-by-step, readily reproducible protocols, and vital notes on troubleshooting and avoiding known pitfalls. Resourceful and easy to follow, In Silico Tools for Gene Discovery seeks to facilitate scientists with further key research on locus mapping, to accelerate gene identification, and to help ascertain the functionality of DNA variation.
In Situ Bioavailability and Toxicity of Organic Chemicals in Aquatic Systems (Methods in Pharmacology and Toxicology)
by Thomas-Benjamin Seiler Markus BrinkmannThis detailed volume examines the complex study of the assessment of in situ bioavailability and toxicity of organic chemicals in aquatic systems with a toolbox of reliable techniques. Beginning with a section on approaches for chemical analytical and bioanalytical techniques in bioavailability research, the book continues with methods to monitor effects in situ and conduct bioassays to assess the effects of complex environmental samples. It concludes with descriptions of various computational models. Written for the Methods in Pharmacology and Toxicology series, chapters feature the kind of expert implementation advice that leads to greater success in the field. Authoritative and versatile, In Situ Bioavailability and Toxicity of Organic Chemicals in Aquatic Systems serves as an ideal guide to aid in tackling the challenge of analyzing and understanding chemical pollution in aquatic systems.
In Situ Hybridization Protocols
by K. H. ChooThirty-one detailed, practical chapters many by the originators themselves present easy-to-follow protocols of in situ hybridization techniques for mapping DNA sequences onto chromosomes, quantitation/localization of RNA in tissues, and detection of virus nucleic acids. The methods serve molecular biologists in most areas of basic and applied research, and in every sort of organism ranging from viruses to Drosophila to humans. The expert contributors provide step-by-step instructions for the preparation of probes for DNA, teleomeric repeats, cDNA sequences, small synthetic oligomers, whole cosmids, mega-size YACs, and chromosome-specific paints from somatic cell hybrids and flow-sorted chromosomes. They also present radioactive and nonradioactive probe labeling procedures with the use of nick translation, random priming, multicolor labeling, PRINS labeling of DNA and RNA, and PCR in situ hybridization. Color illustrations enhance the text. Troubleshooting tips, alternative ways of doing things, and informative explanations of why certain steps are done -- aids not usually found in standard journal recipes -- guarantee a significant difference in the outcome of experiments.
In The Blood
by Don DonaldsonSurviving leukemia, Dr. Holly Fisher is ready to have a child using eggs that were saved before she underwent the fertility-killing treatment. To her horror, she learns her eggs have been stolen from the fertility clinic, taken by a man posing as her lawyer. Holly vows to track the man down, never realizing her attempts will put her in the middle of a medical nightmare.
In This Faulty Machine: A Memoir of Loss and Transformation
by Kathy PageAn exquisite memoir of the author&’s life since being diagnosed with Parkinson&’s disease—informative, funny, and moving all at once.Acclaimed novelist Kathy Page had just completed a promotional tour for her award-winning book Dear Evelyn when a fall during a hike injured her hand. That relatively minor accident seemed to set in motion a cascade of other seemingly unrelated physical issues. Many months of appointments, and of waiting for appointments (including during the COVID lockdown) ensued, until the day that pulled everything together in a single, frightening diagnosis: Parkinson's disease.In This Faulty Machine is an eye-opening, often lyrical and very funny report from that "other kingdom" of illness, from an observant, wise, and honest involuntary resident. The author's acute yet welcoming voice draws us into the erratic, intimate, and troubling effects of the disease and its impact on her relationships alongside the pleasures of family, friends, reading, writing, and the natural world. This one-of-a-kind memoir offers a thoughtful exploration of the complex and evolving science of this debilitating disease, a gripping account of the various ways that it impacts both PWP (People with Parkinson's) and their families. The book is also a unique look into the creative process of a life-long novelist who finds that she can no longer create in the way she has for decades.Wise and warm, the book makes an important contribution to the understanding of Parkinson's disease but is also a rich and heartfelt memoir of creativity and a life being well-lived, even as the challenges mount. It is a story of vital interest to us all as we face our own fragility, and indeed, mortality.
In This Together: My Story
by Ann RomneyNew York Times Bestseller: Ann Romney’s “breezy and frank” memoir of her MS diagnosis and her husband’s unwavering support through the struggle (The Washington Post).A lot of people talk about a transformation that happens when life throws you a curve ball, and the big one in my life was my MS diagnosis. . . . In sharing my story, I want to give others hope as I’ve been given hope on this journey.—Ann RomneyWhen Mitt and Ann Romney met in their late teens, a great American love story began. And their life together would be blessed: five healthy sons, financial security, and a home filled with joy. Then, in 1998, Ann was diagnosed with multiple sclerosis.She couldn’t believe it was real; the disease had no cure at that time—and it still doesn’t. Mitt told her that day that they would tackle the diagnosis as a team: They were in it together. “If you have to be in a wheelchair,” he told her, “I’ll be right there to push it.” And Ann thought, “But I’ll be the one in the wheelchair.”A caregiver and helper her whole life, she’d crossed a terrible invisible line. Instead of caring for her family, she was now the patient. Ann and Mitt would go on to face the most frightening and humbling experience of their lives.From reflections on her early life, her marriage, and her diagnosis and recovery to the sources of her faith and the stories of others who overcame adversity and inspired her to keep going, In This Together is a brave and deeply honest portrait of a family facing an unexpected blow, often in the most public of circumstances.
In Time and with Love
by Marilyn SegalWritten in a jargon-free, parent-friendly style, this generously illustrated book is filled with advice on such topics as nursing, feeding, dressing, interacting with siblings, discipline, and social skills development of handicapped children. Includes a section on games and activities that encourage motor and language skills, and advice on play and care for toddlers and older children.
In Two Minds: Stories of murder, justice and recovery from a forensic psychiatrist
by Dr Sohom Das'thought provoking'Gwen AdsheadShocking, eye-opening and grimly fascinating, these are the true stories, patients and cases that have characterised a career spent treating mentally disordered offenders.As a forensic psychiatrist, it's Dr Das's job to treat and rehabilitate what the tabloids might call the 'criminally insane', many of whom assault, rob, rape, and even kill. His work takes him to high-security prisons and securely locked hospital wards across the country, as well as inside courtrooms, giving evidence as an expert witness.From the young woman who smothered her two-year-old nephew in a flash of psychosis, to the teenager who set his house on fire with his mother locked inside, Dr Das must delve into the minds of these violent offenders to elicit their symptoms of mental illness, understand their actions and prevent future atrocities.In this honest, revealing and at times humorous memoir, Dr Das shares stories from his fifteen years as a psychiatric doctor working with this dangerous clientele, detailing some of his most extreme, heart-breaking and bizarre cases - and how he's learned to live with his mistakes when the worse happens.Compelling, enlightening and candid, if you enjoyed Unnatural Causes, Dark Side of the Mind or The Prison Doctor, you'll love IN TWO MINDS.
In Two Minds: Stories of murder, justice and recovery from a forensic psychiatrist
by Dr Sohom Das'thought provoking'Gwen AdsheadShocking, eye-opening and grimly fascinating, these are the true stories, patients and cases that have characterised a career spent treating mentally disordered offenders.As a forensic psychiatrist, it's Dr Das's job to treat and rehabilitate what the tabloids might call the 'criminally insane', many of whom assault, rob, rape, and even kill. His work takes him to high-security prisons and securely locked hospital wards across the country, as well as inside courtrooms, giving evidence as an expert witness.From the young woman who smothered her two-year-old nephew in a flash of psychosis, to the teenager who set his house on fire with his mother locked inside, Dr Das must delve into the minds of these violent offenders to elicit their symptoms of mental illness, understand their actions and prevent future atrocities.In this honest, revealing and at times humorous memoir, Dr Das shares stories from his fifteen years as a psychiatric doctor working with this dangerous clientele, detailing some of his most extreme, heart-breaking and bizarre cases - and how he's learned to live with his mistakes when the worse happens.Compelling, enlightening and candid, if you enjoyed Unnatural Causes, Dark Side of the Mind or The Prison Doctor, you'll love IN TWO MINDS.
In Two Minds: Stories of murder, justice and recovery from a forensic psychiatrist
by Dr Sohom DasShocking, eye-opening and grimly fascinating, these are the true stories, patients and cases that have characterised a career spent treating mentally disordered offenders.Listen to the end of the audiobook for an exclusive bonus chapter, written and read by the author.As a forensic psychiatrist, it's Dr Das's job to treat and rehabilitate what the tabloids might call the 'criminally insane', many of whom assault, rob, rape, and even kill. His work takes him to high-security prisons and securely locked hospital wards across the country, as well as inside courtrooms, giving evidence as an expert witness.From the young woman who smothered her two-year-old nephew in a flash of psychosis, to the teenager who set his house on fire with his mother locked inside, Dr Das must delve into the minds of these violent offenders to elicit their symptoms of mental illness, understand their actions and prevent future atrocities.In this honest, revealing and at times humorous memoir, Dr Das shares stories from his fifteen years as a psychiatric doctor working with this dangerous clientele, detailing some of his most extreme, heart-breaking and bizarre cases - and how he's learned to live with his mistakes when the worse happens.Compelling, enlightening and candid, if you enjoyed Unnatural Causes, Dark Side of the Mind or The Prison Doctor, you'll love IN TWO MINDS.
In Utero Diagnosis of Skeletal Disorders An Atlas of Prenatal Sonographic and Postnatal Radiologic Correlation
by Jacob Bar-Ziv Israel MeiznerIn Utero Diagnosis of Skeletal Disorders is a unique compendium of current information and 350 original ultrasound images covering the physiology and pathophysiology of osteogenesis. Syndromes are presented schematically and describe the mode of inheritance, definition and morphology, sonographic and radiologic manifestations, and associated malformations. The book is an excellent reference for obstetricians, sonographers, radiologists, and anyone dealing with the early detection of fetal malformations.
In Utero Pediatrics: Research & Practice
by Kun SunThis book is to provide readers with an overview of in utero Pediatrics, an interdisciplinary medicine focusing on sequential and comprehensive care for fetuses and children who have functional and/or structural disorders originated from in utero. It covers congenital disorders in cardiology, neurosurgery, urology, general surgery, endocrine genetics, and other related topics. Each chapter starts with the basic theory, illustrates clinical practices on certain congenital disorders, and summaries recent research and advances in the field. Written by experts with wealthy experiences, this case-based book will be a valuable reference for pediatricians and perinatologists, as well as those who are interested in this field.
In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development
by Dhanya Sunil Pooja R Kamath Raghu Chandrashekhar HThis comprehensive and useful handbook represents a definitive up-to-date compendium of key in vitro bioassay methods that are employed to quantify and validate the anticancer activity of a drug candidate before it makes its way in to animal or clinical trials. <P><P>In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development covers the screening and evaluation of potential drug candidates in a wide category of anticancer assays demonstrating the specific ways in which various pharmaceutical bioassays interpret the activity of drug molecules. The major emphasis of the book is to present those bioassays which can be readily set up and practiced in any laboratory with limited funds, facilities or technical know-how.
In Vitro Diagnostic Industry in China
by Haibo Song Jian’er YaoThis book systematically describes the achievements and current situation of in vitro diagnostic (IVD) industry in China. It consists of eight parts, including the overview on the IVD industry in China in 2019, hot technologies and products of IVD industry, academic, technological and product development in the field of IVD, such as biochemical diagnosis, immune-diagnosis, molecular diagnosis, blood and body fluid diagnosis, microbial detection, point-of-care testing, laboratory assembly line, etc. This book is compiled by an editorial committee composed of well-known entrepreneurs, experts and professors in IVD industry in China. It is a reference book for practitioners of IVD industry, medical laboratory and medical staffs all over the world.
In Vitro Diagnostic Industry in China
by Haibo Song Lizhong DaiThis book systematically describes the achievements and current situation of in vitro diagnostic (IVD) industry in China. It consists of twelve parts, including the overview on the IVD industry in China in 2021, hot technologies and products of IVD industry, academic, technological and product development in the field of IVD, such as biochemical diagnosis, immune-diagnosis, point-of-care testing, molecular diagnosis, blood and body fluid diagnosis, microbial detection, laboratory assembly line, etc.In this second edition, the new contents added include the development of new coronavirus molecular diagnostic products, flight mass spectrometry, tandem mass spectrometry, tumor markers, ELISA immune reagents, autoimmune diagnostics, concomitant diagnosis, fecal and intestinal microecology detection, pathological diagnosis, raw materials for in vitro diagnostic reagents, standard substances and quality controls for in vitro diagnostic reagents, etc., making the content of the whole book more novel and rich.This book is compiled by an editorial committee composed of well-known entrepreneurs, experts and professors in IVD industry in China. It is a reference book for practitioners of IVD industry, medical laboratory and medical staffs all over the world.
In Vitro Fertilization
by Catherine Racowsky Elizabeth S. GinsburgIn Vitro Fertilization: A Comprehensive Guide represents the next frontier in the integrated clinical and laboratory practice of ART. Uniquely balanced, the book provides the necessary understanding of the key laboratory techniques involved in ART and the complex aspects of clinical care. Featuring the contributions of world leaders in the field, the guide begins with the evaluation of the infertile woman and man. It then moves on to discuss treatment protocols and adjunctive treatments. Techniques for oocyte retrieval, insemination, and culture, and embryo transfer and cryopreservation are explored in detail. This comprehensive book covers all aspects of IVF and includes special sections on oocyte and embryo donation as well as gestational carriers, making it ideal for practicing clinicians. Advances such as pre-implantation genetic diagnosis and screening, in vitro maturation, and means of fertility preservation, including oocyte and ovarian tissue cryopreservation, are also covered. The book concludes with dedicated chapters on quality assessment and improvement, regulation and legal concerns, and stress and ART. Readers will gain complete in-depth knowledge of the most current therapies and techniques, enabling optimal care and counsel of their infertile patients.
In Vitro Fertilization: A Textbook of Current and Emerging Methods and Devices
by Ashok Agarwal Alex C. Varghese Zsolt Peter NagyNow in its revised and expanded second edition - including over 20 new chapters - this comprehensive textbook remains a unique and accessible description of the current and developing diagnostic and treatment techniques and technologies comprising in vitro fertilization (IVF). Arranged thematically in sections, each chapter covers a key topic in IVF in a sensible presentation. Parts one and two describe the planning, design and organization of an ART unit and IVF laboratory and equipment and systems, respectively. The sections that follow provide detailed descriptions of IVF techniques, embryo culture methods, sperm processing and selection, insemination procedures, micromanipulation, embryo evaluation, cryopreservation, and embryo transfer. Concluding sections address issues of management and regulation of ART labs across the globe, as well as special topics and emerging techniques and devices. Chapter authors, all experts in the field, contribute their expertise from around the world.With the addition of learning key points and review questions at the beginning and end of each chapter, this new edition of In Vitro Fertilization is a readily accessible, high quality instructional resource for reproductive medicine trainees at all levels. Practicing reproductive endocrinologists, urologists, and embryologists also will find value in the book, as will infertility researchers.
In Vitro Methods in Developmental Toxicology: Use in Defining Mechanisms and Risk Parameters (Routledge Revivals)
by Gary L. Kimmel Devendra M. KochharFirst Published in 1990, this text brings together the work of several investigators who used in vitro techniques to probe the nature of embryonic cell response to a diverse group of chemical agents. It focuses on the approaches, limitations, usefulness and applicability of defining mechanisms underlying altered development. This work includes discussions on pharmacodynamic principles and placental transfer mechanisms. It also examines the potential for in vitro systems to serve as an adjunct to the risk assessment process. Ideal for both the layman and the expert in this area, this volume provides a general understanding of the types of approaches that can be used and specific approaches in investigating altered development.
In Vitro Models for Stem Cell Therapy: Methods and Protocols (Methods in Molecular Biology #2269)
by Bruno Christ Peggy StockThis volume looks at in vitro disease models representing the respiratory, hepatobiliary, osteochondral, nervous, dermal, ocular, immune system, and pathological biological processes like tumorigenesis for stem cell research. The chapters in this book cover a range of diseases and application of various stem cells such as adult stem cells and iPS. Chapters also discuss new methods to characterize and manipulate stem cells with the aim to better understand and improve their biological performance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, In Vitro Models for Stem Cell Therapy: Methods and Protocols is a valuable resource for researchers and scientists interested in learning more about this exciting field
In Vitro Mutagenesis Protocols, 2nd Edition
by Jeff BramanHands-on researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. The contributors focus on improvements to conventional site-directed mutagenesis, including a chapter on chemical site-directed mutagenesis, PCR-based mutagenesis and the modifications that allow high throughput mutagenesis experiments, and mutagenesis based on gene disruption (both in vitro- and in situ-based). Additional methods are provided for in vitro gene evolution; for gene disruption based on recombination, transposon, and casette mutagenesis; and for facilitating the introduction of multiple mutations. Time-tested and highly practical, the protocols in In Vitro Mutagenesis Protocols, 2nd Edition offer today's molecular biologists reliable and powerful techniques with which to illuminate the proteome.
In Vitro Mutagenesis Protocols, 3rd Edition
by Jeff BramanIn the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.<P><P> Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological implications.
In Vitro Neurochemical Techniques
by Alan A. Boulton Glen B. Baker Alan N. BatesonThis collection of state-of-the-art techniques encompasses the breadth of neurochemical and molecular neurobiology research, ranging from the isolation of neuronal genes and the study of their expression to the analysis of receptor-ligand interactions, to the characterization of the consequences of receptor activation. The methods include electrophysiological techniques to explore the functional properties of receptors present in the membranes of excitable cells, methods to isolate novel genes central to neurobiological processes, and protocols to perform in situ hybridization histochemistry. Other methods cover measuring changes in gene expression, the rapid identification of gene polymorphisms, and the identification and characterization of second messenger pathways. Together with its companion volumes, In Vivo Neuromethods and Cell Neurobiology Techniques, all three cutting-edge works will prove exceptionally useful to those basic and clinical neuroscientists who want to expand the range of their current research or develop competence in complementary methods.
In Vitro Neuronal Networks: From Culturing Methods to Neuro-Technological Applications (Advances in Neurobiology #22)
by Monica Frega Michela Chiappalone Valentina PasqualeThis book provides a comprehensive overview of the incredible advances achieved in the study of in vitro neuronal networks for use in basic and applied research. These cultures of dissociated neurons offer a perfect trade-off between complex experimental models and theoretical modeling approaches giving new opportunities for experimental design but also providing new challenges in data management and interpretation. Topics include culturing methodologies, neuroengineering techniques, stem cell derived neuronal networks, techniques for measuring network activity, and recent improvements in large-scale data analysis. The book ends with a series of case studies examining potential applications of these technologies.
In Vitro Neurotoxicology: Methods and Protocols
by Gennaro Giordano Lucio G. Costa Marina GuizzettiIn recent years, the need to develop acceptable alternatives to conventional animal testing for neurotoxicity and developmental neurotoxicity has been increasingly recognized, and much effort is being directed toward the development of alternative models, utilizing mostly mammalian cells in culture but also non-mammalian model systems. In Vitro Neurotoxicology: Methods and Protocols presents a series of cellular, biochemical, and molecular methodological protocols in the area of in vitro neurotoxicology, with an emphasis on mammalian cell culture systems. Opening with a section on methodologies for preparing several cellular systems of variable complexity, amenable for in vitro neurotoxicological studies, the thorough volume continues with coverage of methods to measure cellular death and major mechanisms, methods for assessing mechanisms of nervous system cell toxicity related to impairment of cell signaling, while a final section illustrates additional methods for assessing important nervous system processes such as cell proliferation, neuritogenesis, and synaptogenesis. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, In Vitro Neurotoxicology: Methods and Protocols serves researchers with an interest in assessing or characterizing the potential neurotoxicity of environmental contaminants, drugs, or other chemicals.