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In Vitro Neurotoxicology: Principles and Challenges
by Evelyn Tiffany-CastiglioniNeurotoxicity assessment with in vitro systems offers not only a means to better understand mechanisms of action of toxicants and decrease reliance on laboratory animals, but also provides a basis for reliable, high throughput screening of new and untested chemicals. In In Vitro Neurotoxicology: Principles and Challenges, well-respected leaders in the field take a fresh look at their own and others' work, critically and comparatively analyzing it across experimental systems and toxicants, and synthesizing essential principles for in vitro neurotoxicity testing. In thematically organized chapters, the authors employ examples comprised of multiple experimental systems and endpoints, and discuss the use of complementary in vivo/in vitro strategies. Neurotoxicants of significance to human health are emphasized, especially those for which metabolism and dose-responses are well studied both in vivo and in vitro: lead, mercury, organophosphorus insecticides, polychlorinated biphenyls and dioxin, ethanol, and endogenous proteins. Several neurotoxicology topics covered provide insight into neurodevelopmental and neurodegenerative diseases, including the concept of astroglia as depots for lead and other metals in the central nervous system, the elucidation of factors involved in the onset of puberty in females, and the exploration of endogenous proteins as neurotoxicants. <P><P> Authoritative and forward-looking, In Vitro Neurotoxicology: Principles and Challenges sets out new concepts and research directions that will facilitate significant improvement in today's methods and systems for in vitro neurotoxicity testing.
In Vitro Percutaneous Absorption: Principles, Fundamentals, and Applications
by Howard I. Maibach Robert L. BronaughThis book covers all major areas of interest in the rapidly expanding field of in vitro methods for percutaneous absorption studies. Specific areas discussed include diffusion cell design, receptor fluid, preparation of skin, and temperature. The book covers experimental methodology, as well as the underlying principles and fundamentals that help professionals and students gain an understanding of the basis for currently used methodology.
In Vitro Toxicity Testing Protocols
by Christopher K. Atterwill Sheila O’hareIn Vitro Toxicity Testing Protocols is a collection of proven, state-of-the-art methods that toxicologists can use in carrying out laboratory studies. The book's expert contributors offer a variety of efficient in vitro toxicology techniques that can be applied regularly in your lab, or can serve as the fundamental basis for the establishment of novel techniques in working laboratories. All the procedures are highly reliable. Also included are ex vivo techniques, whose development embraces the twin aims of reduction and refinement of the use of laboratory animals; the editors believe this is an important step toward the ethical treatment of laboratory animals. In keeping with the successful format of preceding volumes of the Methods in Molecular Biology series, concise, easy-to-follow, step-by-step protocols are provided for each technique, together with extensive notes that cover troubleshooting and offer special tips to ensure the successful completion of your experiments.
In Vitro Toxicology Systems
by Paul Jennings Anna Bal-PriceIn Vitro Toxicology Systems brings together important issues and considerations needed in order to develop a workable, reliable, integrated testing strategy for the replacement of animals in toxicity testing regimes. This thorough volume includes sections on in vitro models for systemic organ toxicity, neurotoxicity, sensory organs, immunotoxicity and reproductive toxicity and addresses how stem cells may be used going forward. The book also tackles difficult areas of toxicology such as carcinogenicity and nanotoxicology, with additional chapters dedicated to kinetics, metabolism, and in vitro in vivo extrapolation. The book also addresses biological processes such as stress response pathways and mechanistic biomarkers and how these can be uncovered and measured using high content approaches. Reliable and authoritative, In Vitro Toxicology Systems will be of benefit not only to students, scientists, and regulators working in the field of chemical safety assessment but also to a wider scientific audience. --
In Vitro Transcription and Translation Protocols
by Guido GrandiA highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. Completely updated with new chapters and topics, there is in-depth analysis of current technologies and applications of in-vitro transcriptions and translations systems. Detailed protocols for protein production using different in vitro transcription and translation systems are included.
In Vivo Cellular Imaging Using Fluorescent Proteins
by Robert HoffmanThe discovery and genetic engineering of fluorescent proteins has revolutionized cell biology. What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in the live animal. This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols is the first volume in the new field of in vivo cell biology and it serves both professionals and novices with its well-honed methodologies.
In Vivo Confocal Microscopy in Eye Disease
by Golshan Latifi Scott HauIn Vivo Confocal Microscopy in Eye Disease is a comprehensive new text that covers the latest advances in the field of in vivo confocal imaging. It presents a detailed overview of the basic anatomy of the different part of the cornea, conjunctiva and adnexal structures. It discusses the use of in vivo confocal microscopy in a range of clinical applications including the diagnosis of infective keratitis, corneal dystrophies, cornea nerves, conjunctival diseases and their differentiating features. Numerous confocal images, clinical pictures, other paraclinical images and histopathology slides of different ocular pathologies are examined and presented throughout the text. This book systematically reviews the use of confocal imaging in basic science of ocular tissues to the diagnosis of eye disease assembled in a single volume. It provides an up to date resource with an in depth review of scientific literature relating to both the clinical and research application of in vivo confocal microscopy in eye disease. The book ends with a discussion on non-ocular application of in vivo confocal microscopy and future developments including other emerging imaging technologies. This text will provide an invaluable resource for those who are interested in ocular imaging and it represents a timely resource for all practicing and trainee ophthalmologists
In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs
by Shinichi Ohno Nobuhiko Ohno Nobuo TeradaThis book focuses on actual morphofunctional findings of cells and tissues in living animal organs. Medical and biological scientists need to know the real in vivo morphology and immunolocalization of the molecular components in living animal organs. Recently, the live imaging of cells and tissues of animals with fluorescence-labeled proteins by gene manipulation has become more and more popular in biological fields. Current research, meanwhile, has revealed that immunohistochemical or morphological studies exclusively depend on living animal organs. The cryotechnique is one of the most useful tools for immunohistochemistry and bioimaging of animal organs. This book describes the epoch-making cryotechnique originally developed by the editors. The book also makes the management of living animal morphology more accessible not only for biomedical researchers but also for clinical doctors, providing a valuable resource work on the current perspectives of in vivo morphology.
In Vivo Inhalation Toxicity Screening Methods for Manufactured Nanomaterials (Current Topics in Environmental Health and Preventive Medicine)
by Toru Takebayashi Robert Landsiedel Masashi GamoThis edited volume discusses the short-term inhalation study (STIS) and intratracheal administration, the two major in vivo inhalation-toxicity screening methods, which play an important role in efficient hazard evaluation. It also provides a general overview of the inhalation toxicity of nanomaterials and related issues. For each screening method, it provides up-to-date information on the test procedures, interpretation of the test results, useful applications, and related technologies. In view of the increasing variety of nanomaterials in practical use, the book offers a basis for building a framework for grouping and read-across assessments of nanomaterials. With contributions by academic and industrial experts, In vivo Inhalation Toxicity Screening Methods for Manufactured Nanomaterials is a pragmatic reference resource for readers who are responsible for assessing the safety of nanomaterials in R&D and business, as well as researchers.
In Vivo MR Techniques in Drug Discovery and Development
by Nicolau BeckmannImaging technologies are receiving much attention in the pharmaceutical industry because of their potential for accelerating drug discovery and development. Magnetic Resonance (MR) Imaging is one of the principal modalities since it allows anatomical, functional, metabolic, and to a certain extent even target-related information to be gathered in v
In Vivo Neuromethods
by Alan A. Boulton Glen B. Baker Alan N. BatesonA superb collection of time-tested, key techniques for investigating nervous system function, as well as the actions of drugs on the nervous system. The methods range from authoritative treatment of such well-established techniques as recording electrical activity in the brain, to practical coverage of state-of-the-art methods, including neural transplantation and antisense technology. The techniques described are particularly suitable for studying the mechanisms of action of psychotropic drugs and drugs of abuse, as well as the etiology and pharmacotherapy of neuropsychiatric diseases and neurodegenerative disorders. Several of the methods detailed are highly useful in screening potential drugs for the treatment of depression, anxiety disorders, schizophrenia, and Parkinson's and Alzheimer's disease.
In Vivo Neuropharmacology and Neurophysiology
by Athineos PhilippuThis volume presents classical approaches to in vivo neuropharmacology and neurophysiology, such as c-fos, electrochemistry, microdialysis microstimulation, and push-up superfusion. It also explores exciting new methods for behavioral analysis, and techniques based on optogenetics and non-invasive magnetic resonance imaging. The chapters of this book cover topics such as principles of stereotaxy, pharmaco-based fMRI and neurophysiology in humans and non-human primates, electrical nerve stimulation and central microstimulation, involvement of neurotransmitters in mnemonic processes, the impact of cannabinoids on motor activity, as well as the involvement of nitric oxide in neurotoxicity produced by psychostimulant drugs. Each chapter also discusses difficulties, tips, tricks, and precautions to take. Neuromethods series style chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.Cutting-edge and practical, In Vivo Neuropharmacology and Neurophysiology is a valuable resource for experienced and less experienced investigators of brain function and brain disorders.
In Vivo Optical Imaging of Brain Function
by Ron D. FrostigThese are exciting times for the field of optical imaging of brain function. Rapid developments in theory and technology continue to considerably advance understanding of brain function. Reflecting changes in the field during the past five years, the second edition of In Vivo Optical Imaging of Brain Function describes state-of-the-art techniques a
In Vivo Reprogramming in Regenerative Medicine
by Açelya YilmazerThis new volume reviews current progress on different approaches of in vivo reprogramming technology. Leaders in the field discuss how in vivo cell lineage reprogramming can be used for tissue repair and regeneration in different organs, including brain, spinal cord, pancreas, liver and heart. Recent studies on in vivo cell reprogramming towards pluripotency are reviewed; examples are given to show its potential in regenerative medicine. In each chapter, the regenerative potential of different in vivo reprogramming approaches is discussed in detail. More specifically, how different tissue failures or damages can be treated with this technology is explained. Examples from various animal models are given and the regenerative potential of in vivo reprogramming is compared to that of cell transplantation studies. The last chapter discusses current challenges of these preclinical studies and gives suggestions in order to improve the current strategies. Future directions are indicated for the transition of in vivo reprogramming technology to clinical settings. This is among the first books in the literature which specifically focuses on the in vivo reprogramming technology in regenerative medicine and these chapters collectively cover one of the most important and exciting topics of regenerative medicine.
In Vivo Self-Assembly Nanotechnology for Biomedical Applications
by Hao Wang Li-Li LiThis book reviews and discusses the development of self-assembled nanomaterials applied in biomedical fields. Based on self-assembled nanomaterial constructions, it highlights the mechanisms of the stimuli-response-induced assembly/disassembly and transformation. Moreover, it examines healthcare-related diseases, the applications of nanomaterials and therapy/detection strategies, providing readers with both a deeper understanding of the subject and inspirations for future research. The book is primarily intended for researchers and graduate students in the fields of material sciences and chemistry who wish to learn about the principles, methods, mechanisms and biomedical applications of self-assembled nanomaterials.
In a Little Kingdom
by Perry StieglitzFirst Published in 1991. Routledge is an imprint of Taylor & Francis, an Informa company.
In an Instant: A Family's Journey of Love and Healing
by Lee Woodruff Bob WoodruffIn one of the most anticipated books of the year, Lee Woodruff, along with her husband, Bob Woodruff, share their never-before-told story of romance, resilience, and survival following the tragedy that transformed their lives and gripped a nation.In January 2006, the Woodruffs seemed to have it all-a happy marriage and four beautiful children. Lee was a public relations executive and Bob had just been named co-anchor of ABC's World News Tonight. Then, while Bob was embedded with the military in Iraq, an improvised explosive device went off near the tank he was riding in. He and his cameraman, Doug Vogt, were hit, and Bob suffered a traumatic brain injury that nearly killed him.In an Instant is the frank and compelling account of how Bob and Lee's lives came together, were blown apart, and then were miraculously put together again-and how they persevered, with grit but also with humor, through intense trauma and fear. Here are Lee's heartfelt memories of their courtship, their travels as Bob left a law practice behind and pursued his news career and Lee her freelance business, the glorious births of her children and the challenges of motherhood.Bob in turn recalls the moment he caught the journalism "bug" while covering Tiananmen Square for CBS News, his love of overseas assignments and his guilt about long separations from his family, and his pride at attaining the brass ring of television news-being chosen to fill the seat of the late Peter Jennings.And, for the first time, the Woodruffs reveal the agonizing details of Bob's terrible injuries and his remarkable recovery. We learn that Bob's return home was not an end to the journey but the first step into a future they have learned not to fear but to be grateful for.In an Instant is much more than the dual memoir of love and courage. It is an important, wise, and inspiring guide to coping with tragedy-and an extraordinary drama of marriage, family, war, and nation.A percentage of the proceeds from this book will be donated to the Bob Woodruff Family Fund for Traumatic Brain Injury.From the Hardcover edition.
In gesprek met de palliatieve patiënt
by Cilia Linssen A. M. TheIn het najaar van 2003 kwam het beroepscompetentie- en kwalificatieprofiel van de eerstverantwoordelijk verzorgende (EVV) tot stand. Dit profiel vormt de basis van de eerste branche-erkende opleiding voor verzorgenden, de EVV-opleiding. Het boek: De eerstverantwoordelijk verzorgende geeft een theoretische basis aan alle verzorgenden die als EVV of zorgcoördinator (gaan) werken. Het boek informeert u over de dertien beroepscompetenties van de EVV en is door deze volledigheid uniek in zijn soort. Thema's die behandeld worden zijn: de zorgvrager centraalmethodisch werkencoördinatie en continuïteit van zorgkwaliteitszorg en professionaliseringwonen en leven in een groep
In the Blink of an Eye
by Andrew W. ArtensteinThis book is an account of a major historical event, in the world of medicine. As the son of one of the lead scientists who developed the vaccine for meningococcal meningitis, Andrew Artenstein has a unique perspective on the story. In the Blink of an Eye shares his experience.
In the Blood: How Two Outsiders Solved a Centuries-Old Medical Mystery and Took On the US Army
by Charles BarberThe "high-stakes" true story of how an absent-minded inventor and a down-on-his-luck salesman joined forces to create a once‑in‑a‑generation lifesaving product: "Suspenseful storytelling helps us see and feel the struggle and frustration, the sweat and tears . . . Inspiring&” (Robert Kolker, #1 New York Times bestselling author of Hidden Valley Road). At the 1993 Battle of Mogadishu, dramatized by the popular film Black Hawk Down, the majority of soldiers who died were killed instantly or bled to death before they could reach an operating table. This tragedy reinforced the need for a revolutionary treatment that could transform trauma medicine. So, when Frank Hursey and Bart Gullong—who had no medical or military experience—discovered that a cheap, crushed rock called zeolite had blood‑clotting properties, they brought it to the military's attention. The Marines and the Navy adopted the resulting product, QuikClot, immediately. The Army, however, resisted. It had two products of its own being developed to prevent excessive bleeds, one of which had already cost tens of millions of dollars. The other, "Factor Seven," had a more dangerous complication: its side effects could be deadly. Unwilling to let its efforts end in failure—and led by the highly influential surgeon Colonel John Holcomb—the Army set out to smear QuikClot&’s reputation. Over the course of six years, Hursey and Gullong engaged in an epic struggle with Holcomb for recognition. Ultimately, a whistle‑blower inside the Army challenged the Army&’s embrace of Factor Seven, which resulted in a massive lawsuit led by the U.S. Department of Justice. The lawsuit focused further attention on the financial ties between the pharmaceutical company that produced Factor Seven and Holcomb&’s research institute. By withholding QuikClot—which later became the medical miracle of the Iraq War—and in the use of Factor Seven with its known, life-threatening risks of heart attacks and strokes, the lives of countless soldiers were imperiled. Using deep reportage and riveting prose, In the Blood recounts this little‑known David‑and‑Goliath story of corruption, greed, and power within the military—and the devastating consequences of unchecked institutional arrogance.
In the Body of the World: A Memoir of Cancer and Connection
by Eve EnslerThe bestselling author of The Vagina Monologues shares her “extraordinarily riveting, graphic story of survival” (Publishers Weekly).In this extraordinary and evocative memoir, playwright, author, and activist V, formerly Eve Ensler, traces many paths of reconnection: with her body, after she is diagnosed with cancer; with the people of the world, in the face of injustice and abuse; and with the earth, victim of mass exploitation.Working in the Congo, V meets survivors of horrific rape and violence and sees firsthand how these women are creating hope and possibility out of horror. Just as she is about to help open a revolutionary leadership center called City of Joy, she is diagnosed with uterine cancer, resulting in months of difficult treatment. Through her experience, V is forced to become first and foremost a body—pricked, punctured, cut, scanned. As she recovers from her illness, V is able to let go of everything that doesn’t matter and find strength in what does.In the Body of the World is a haunting, revelatory work that calls on us to reestablish our connection to our bodies, to the world, and to those around us.Praise for In the Body of the World“Warm, funny, furious, and astute, as well as poetic, passionate, and heroic, Ensler harnesses all that she lost and learned to articulate a galvanizing vision of the essence of life: ‘The only salvation is kindness.’ . . . [A] scorching and enlightening memoir.” —Booklist (starred review)“A necessary book to read for its fierce, passionate commitment to making the world a safe place for women.” —The Boston Globe
In the Company of Grace: A Veterinarian's Memoir of Trauma and Healing
by Jody LulichThe son of a Black mother and white father overcomes family trauma to find the courage of compassion in veterinary practice Rising to accept a prestigious award, Jody Lulich wondered what to say. Explain how he&’d been attracted to veterinary medicine? Describe how caring for helpless, voiceless animals in his own shame and pain provided a lifeline, a chance to heal himself as well? Lulich tells his story in In the Company of Grace, a memoir about finding courage in compassion and strength in healing—and power in finally confronting the darkness of his youth.Lulich&’s white father and Black mother met at a civil rights rally, but love was no defense against their personal demons. His mother&’s suicide, in his presence when he was nine years old, and his sometimes brutal father&’s subsequent withdrawal set Lulich on a course from the South Side of Chicago to the Tuskegee School of Veterinary Medicine in Alabama to an endowed chair at the University of Minnesota, forever searching for the approval and affection that success could not deliver. Though shadowed by troubling secrets, his memoir also features scenes of surprising light and promise—of the neighbors who take him in, a brother&’s unlikely effort to save Christmas, his mother&’s memories of the family&’s charmed early days, bright moments (and many curious details) of veterinary practice. Most consequentially, at Tuskegee Lulich rents a room in the home of a seventy-five-year-old Black woman named Grace, whose wholehearted adoption of him—and her own stories of the Jim Crow era—finally gives him a sense of belonging and possibility.Completing his book amid the furor over George Floyd&’s murder, Lulich reflects on all the ways that race has shaped his life. In the Company of Grace is a moving testament to the power of compassion in the face of seemingly overwhelming circumstances.
In the Company of My Sisters: My Story, My Truth
by Karen Eubanks JacksonDance as though no one is watching you Love as though you have never been hurt Sing as though no one can hear you Live as though heaven is here on earth - Anonymous For millions of women, the words "You have breast cancer" are a harsh reality. For Black women, those words hit especially hard as the mortality rate for Black women is staggering. It's a battle Karen Eubanks Jackson knows all too well. A FOUR-time breast cancer survivor, Jackson has used her triumphs, tragedies and lessons learned to inspire others. Now, she’s sharing it all in this evocative new book, In the Company of My Sisters. First diagnosed in 1993, Jackson knew little about the disease, despite the fact that she’d had a relative die from breast cancer. It was, after all, a silent shame the family kept. Determined to educate herself, Jackson devoured herself in research. And when she couldn’t find information or support specifically for Black women, she set out to fill that void, creating Sisters Network Inc., the only national survivorship-run organization designed to increase local and national attention to the devastating impact that breast cancer has in the Black community. In the Company of My Sisters is Jackson’s story. Her truth in battling the disease for 27 years. How she survived. How she succeeded and built a national sisterhood of Black breast cancer survivors. How she hopes to continue inspiring and educating others along the way. The book is filled with personal experiences and observations from Jackson as well as other survivors, this book will give insight into what shaped Jackson into a woman able to beat breast cancer – again and again and again. And with all the valuable information Jackson has accumulated over the years, the book will also serve as a one-stop resource for all your breast cancer questions. For those battling breast cancer, those walking the journey with survivors, and those who just want to educate themselves, In the Company of My Sisters will inspire you by the beauty and strength of the human spirit and why Jackson has made it her life’s mission change the course of the narrative surrounding the disease and help Black women take action to beat breast cancer.
In the Footsteps of the Yellow Emperor
by Peter EckmanAcupuncture now enjoys immense popularity as a major form of alternative healing in the West. Once considered mysterious and exotic, acupuncture dates from before the first century BC and the Huang Di Nei Jing (The Yellow Emperor's Internal Classic), one of ancient China's earliest philosophical and medicinal texts. What makes it so popular today? How did the traditions of acupuncture evolve, migrate, and adapt to different societies worldwide?Originally published in 1996 to great acclaim, this updated paperback edition of In the Footsteps of the Yellow Emperor offers a fascinating and highly readable account of one doctor's personal and professional quest to explore the healing traditions of Asia, its rich historical lineage, and its subsequent transmission to contemporary Europe and North America. It is a must-have book in the healing arts for all practitioners and general-interest readers. Peter Eckman, MD, PhD (New York University), MAc (College of Traditional Acupuncture, Leamington Spa, England) is editor of The Essential Book of Traditional Chinese Medicine and co-author of Closing the Circle: Lectures on the Unity of Traditional Oriental Medicine. He has a medical practice in San Francisco.
In the Highest Traditions of the Royal Navy: The Life of Captain John Leach MVO DSO
by Matthew B WillsOn 10 December 1941, the Royal Navy battleship HMS Prince of Wales was sunk by Japanese bombers in the South China Sea. Amongst the several hundred men who went down with her was her Captain, John Leach, who had fought against frightful odds and to the very end made the best of an impossible situation with courage and calmness. He truly embodied ‘the highest traditions of the Royal Navy’. Author Matthew B. Wills analyses the influences that shaped John Leach and led him ultimately to his heroic end: his time at Royal Naval College Osborne and Britannia Royal Naval College Dartmouth and his baptism of fire when he survived a direct shell hit to the bridge where he was standing. He describes Leach’s role in command during the Battle of the Denmark Strait, during which the Prince of Wales inflicted damage on the Bismarck that contributed to her later destruction ? and then the ill-fated mission to Singapore as part of Force Z, an attempt to intercept Japanese landings in Malaya.