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Methodologies for Metabolomics
by Norbert W. Lutz Jonathan V. Sweedler Ron A. WeversMetabolomics, the global characterization of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways, and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites including nuclear magnetic resonance and mass spectrometry are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples, and diseases, ranging from in vivo metabolomics in humans and animals, to in vitro analysis of tissue samples, cultured cells and biofluids.
Methodologies in Biosimilar Product Development (Chapman & Hall/CRC Biostatistics Series)
by Shein-Chung Chow Sang Joon LeeMethodologies for Biosimilar Product Development covers the practical and challenging issues that are commonly encountered during the development, review, and approval of a proposed biosimilar product. These practical and challenging issues include, but are not limited to the mix-up use of interval hypotheses testing (i.e., the use of TOST) and confidence interval approach, a risk/benefit assessment for non-inferiority/similarity margin, PK/PD bridging studies with multiple references, the detection of possible reference product change over time, design and analysis of biosimilar switching studies, the assessment of sensitivity index for assessment of extrapolation across indications without collecting data from those indications not under study, and the feasibility and validation of non-medical switch post-approval. Key Features: Reviews withdrawn draft guidance on analytical similarity assessment. Evaluates various methods for analytical similarity evaluation based on FDA’s current guidelines. Provides a general approach for the use of n-of-1 trial design for assessment of interchangeability. Discusses the feasibility and validity of the non-medical switch studies. Provides innovative thinking for detection of possible reference product change over time. This book embraces innovative thinking of design and analysis for biosimilar studies, which are required for review and approval of biosimilar regulatory submissions.
Methodologies of Multi-Omics Data Integration and Data Mining: Techniques and Applications (Translational Bioinformatics #19)
by Kang NingThis book features multi-omics big-data integration and data-mining techniques. In the omics age, paramount of multi-omics data from various sources is the new challenge we are facing, but it also provides clues for several biomedical or clinical applications. This book focuses on data integration and data mining methods for multi-omics research, which explains in detail and with supportive examples the “What”, “Why” and “How” of the topic. The contents are organized into eight chapters, out of which one is for the introduction, followed by four chapters dedicated for omics integration techniques focusing on several omics data resources and data-mining methods, and three chapters dedicated for applications of multi-omics analyses with application being demonstrated by several data mining methods. This book is an attempt to bridge the gap between the biomedical multi-omics big data and the data-mining techniques for the best practice of contemporary bioinformatics and the in-depth insights for the biomedical questions. It would be of interests for the researchers and practitioners who want to conduct the multi-omics studies in cancer, inflammation disease, and microbiome researches.
Methods In Animal Physiology
by Zdenek DeylThe aim of the present volume was to give an overview over different available methodological approaches. The specialists may, perhaps, object that in their particular field the level of information is superficial. However, let them look at other chapters in which different approaches are discussed and which, surely, will appear less superficial from the more general point of view. We hope, at least, that crucial references can be traced throughout the book that would enable the readers to go in more detail when desired. It can be traced throughout the book that would enable the readers to go in more detail when desired. It was really one of our ideas to draw the survey of possibilities available. If this can stimulate the readers to use ideas to draw the survey of possibilities available. If this can stimulate the readers to use other methods that those they are routinely using the goals will be met.
Methods In Biotechnology
by Hans-Peter SchmauderProvides a grounding in the experimental techniques applicable to the discipline of biotechnology. The introductory section in the text describes procedures for analysis of inorganic and organic materials, strain maintenance and fundamental experiments in gene manipulation. Other chapters deal with fermentation techniques, purification methods for substances of interest, preparation of microbial sensors and the demonstration of oil degradation by bacteria. The final chapter deals with statistical planning of experiments and scale-up methods.
Methods In Medical Ethics
by Daniel P. Sulmasy Jeremy SugarmanMedical ethics draws upon methods from a wide array of disciplines, including anthropology, economics, epidemiology, health services research, history, law, medicine, nursing, philosophy, psychology, sociology, and theology. <p><p> In this influential book, outstanding scholars in medical ethics bring these many methods together in one place to be systematically described, critiqued, and challenged. Newly revised and updated chapters in this second edition include philosophy, religion and theology, virtue and professionalism, casuistry and clinical ethics, law, history, qualitative research, ethnography, quantitative surveys, experimental methods, and economics and decision science. <p><p>This second edition also includes new chapters on literature and sociology, as well as a second chapter on philosophy which expands the range of philosophical methods discussed to include gender ethics, communitarianism, and discourse ethics. In each of these chapters, contributors provide descriptions of the methods, critiques, and notes on resources and training. <p><p> Methods in Medical Ethics is a valuable resource for scholars, teachers, editors, and students in any of the disciplines that have contributed to the field. As a textbook and reference for graduate students and scholars in medical ethics, it offers a rich understanding of the complexities involved in the rigorous investigation of moral questions in medical practice and research.
Methods and Applications of Sample Size Calculation and Recalculation in Clinical Trials (Springer Series in Pharmaceutical Statistics)
by Meinhard KieserThis book provides an extensive overview of the principles and methods of sample size calculation and recalculation in clinical trials. Appropriate calculation of the required sample size is crucial for the success of clinical trials. At the same time, a sample size that is too small or too large is problematic due to ethical, scientific, and economic reasons. Therefore, state-of-the art methods are required when planning clinical trials. Part I describes a general framework for deriving sample size calculation procedures. This enables an understanding of the common principles underlying the numerous methods presented in the following chapters. Part II addresses the fixed sample size design, where the required sample size is determined in the planning stage and is not changed afterwards. It covers sample size calculation methods for superiority, non-inferiority, and equivalence trials, as well as comparisons between two and more than two groups. A wide range of further topics is discussed, including sample size calculation for multiple comparisons, safety assessment, and multi-regional trials. There is often some uncertainty about the assumptions to be made when calculating the sample size upfront. Part III presents methods that allow to modify the initially specified sample size based on new information that becomes available during the ongoing trial. Blinded sample size recalculation procedures for internal pilot study designs are considered, as well as methods for sample size reassessment in adaptive designs that use unblinded data from interim analyses. The application is illustrated using numerous clinical trial examples, and software code implementing the methods is provided. The book offers theoretical background and practical advice for biostatisticians and clinicians from the pharmaceutical industry and academia who are involved in clinical trials. Covering basic as well as more advanced and recently developed methods, it is suitable for beginners, experienced applied statisticians, and practitioners. To gain maximum benefit, readers should be familiar with introductory statistics. The content of this book has been successfully used for courses on the topic.
Methods and Applications of Statistics in Clinical Trials
by N. BalakrishnanA complete guide to the key statistical concepts essential for the design and construction of clinical trialsAs the newest major resource in the field of medical research, Methods and Applications of Statistics in Clinical Trials, Volume 1: Concepts, Principles, Trials, and Designs presents a timely and authoritative reviewof the central statistical concepts used to build clinical trials that obtain the best results. The referenceunveils modern approaches vital to understanding, creating, and evaluating data obtained throughoutthe various stages of clinical trial design and analysis.Accessible and comprehensive, the first volume in a two-part set includes newly-written articles as well as established literature from the Wiley Encyclopedia of Clinical Trials. Illustrating a variety of statistical concepts and principles such as longitudinal data, missing data, covariates, biased-coin randomization, repeated measurements, and simple randomization, the book also provides in-depth coverage of the various trial designs found within phase I-IV trials. Methods and Applications of Statistics in Clinical Trials, Volume 1: Concepts, Principles, Trials, and Designs also features:Detailed chapters on the type of trial designs, such as adaptive, crossover, group-randomized, multicenter, non-inferiority, non-randomized, open-labeled, preference, prevention, and superiority trialsOver 100 contributions from leading academics, researchers, and practitionersAn exploration of ongoing, cutting-edge clinical trials on early cancer and heart disease, mother-to-child human immunodeficiency virus transmission trials, and the AIDS Clinical Trials GroupMethods and Applications of Statistics in Clinical Trials, Volume 1: Concepts, Principles, Trials, and Designs is an excellent reference for researchers, practitioners, and students in the fields of clinicaltrials, pharmaceutics, biostatistics, medical research design, biology, biomedicine, epidemiology,and public health.
Methods and Applications of Statistics in Clinical Trials, Volume 1: Concepts, Principles, Trials, and Designs (Methods and Applications of Statistics)
by N. BalakrishnanA complete guide to the key statistical concepts essential for the design and construction of clinical trials As the newest major resource in the field of medical research, Methods and Applications of Statistics in Clinical Trials, Volume 1: Concepts, Principles, Trials, and Designs presents a timely and authoritative reviewof the central statistical concepts used to build clinical trials that obtain the best results. The referenceunveils modern approaches vital to understanding, creating, and evaluating data obtained throughoutthe various stages of clinical trial design and analysis. Accessible and comprehensive, the first volume in a two-part set includes newly-written articles as well as established literature from the Wiley Encyclopedia of Clinical Trials. Illustrating a variety of statistical concepts and principles such as longitudinal data, missing data, covariates, biased-coin randomization, repeated measurements, and simple randomization, the book also provides in-depth coverage of the various trial designs found within phase I-IV trials. Methods and Applications of Statistics in Clinical Trials, Volume 1: Concepts, Principles, Trials, and Designs also features: Detailed chapters on the type of trial designs, such as adaptive, crossover, group-randomized, multicenter, non-inferiority, non-randomized, open-labeled, preference, prevention, and superiority trials Over 100 contributions from leading academics, researchers, and practitioners An exploration of ongoing, cutting-edge clinical trials on early cancer and heart disease, mother-to-child human immunodeficiency virus transmission trials, and the AIDS Clinical Trials Group Methods and Applications of Statistics in Clinical Trials, Volume 1: Concepts, Principles, Trials, and Designs is an excellent reference for researchers, practitioners, and students in the fields of clinicaltrials, pharmaceutics, biostatistics, medical research design, biology, biomedicine, epidemiology,and public health.
Methods and Biostatistics in Oncology: Understanding Clinical Research as an Applied Tool
by Rachel P. Riechelmann Raphael. L.C AraújoThis book introduces and discusses the most important aspects of clinical research methods and biostatistics for oncologists, pursuing a tailor-made and practical approach. Evidence-based medicine (EBM) has been in vogue in the last few decades, particularly in rapidly advancing fields such as oncology. This approach has been used to support decision-making processes worldwide, sparking new clinical research and guidelines on clinical and surgical oncology. Clinical oncology research has many peculiarities, including specific study endpoints, a special focus on survival analyses, and a unique perspective on EBM. However, during medical studies and in general practice, these topics are barely taught. Moreover, even when EBM and clinical cancer research are discussed, they are presented in a theoretical fashion, mostly focused on formulas and numbers, rather than on clinical application for a proper literature appraisal. Addressing that gap, this book discusses more practical aspects of clinical research and biostatistics in oncology, instead of relying only on mathematical formulas and theoretical considerations. Methods and Biostatistics in Oncology will help readers develop the skills they need to understand the use of research on everyday oncology clinical practice for study design and interpretation, as well to demystify the use of EBM in oncology.
Methods for Analysing and Reporting EQ-5D Data
by David Parkin Nancy Devlin Bas JanssenThis open access book is the first published guide about how to analyse data produced by the EQ-5D, one of the most widely used Patient Reported Outcomes questionnaires world wide. The authors provide practical, clear and comprehensive guidance in five concise chapters. Following an overview of the EQ-5D and its analysis, we describe how the questionnaire data – the EQ-5D profile and EQ VAS – can be analysed in different ways to generate important insights into peoples’ health. We then show how the value sets which accompany the EQ-5D can be applied to summarise patients’ data. The final chapter deals with advanced topics, including the use of Minimally Important Differences, case-mix adjustment, mapping, and more. This book is essential for those new to analyzing EQ-5D data and will be also be valuable for those with more experience. The methods can be applied to any EQ-5D instrument (for example, the three- and five-level and Youth versions) and many of the methods described will be equally relevant to other Patient Reported Outcomes instruments.
Methods for Analyzing Large Neuroimaging Datasets (Neuromethods #218)
by Robert Whelan Hervé LemaîtreThis Open Access volume explores the latest advancements and challenges in standardized methodologies, efficient code management, and scalable data processing of neuroimaging datasets. The chapters in this book are organized in four parts. Part One shows the researcher how to access and download large datasets, and how to compute at scale. Part Two covers best practices for working with large data, including how to build reproducible pipelines and how to use Git. Part Three looks at how to do structural and functional preprocessing data at scale, and Part Four describes various toolboxes for interrogating large neuroimaging datasets, including machine learning and deep learning approaches. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and comprehensive, Methods for Analyzing Large Neuroimaging Datasets is a valuable resource that will help researchers obtain the practical knowledge necessary for conducting robust and reproducible analyses of large neuroimaging datasets.
Methods for Community-Based Participatory Research for Health
by Barbara A. Israel Eugenia Eng Amy J. Schulz Edith A. ParkerThis thoroughly revised and updated second edition of Methods for Community-Based Participatory Research for Health provides a step-by-step approach to the application of participatory approaches to quantitative and qualitative data collection and data analysis. With contributions from a distinguished panel of experts, this important volume shows how researchers, practitioners, and community partners can work together to establish and maintain equitable partnerships using a Community-Based Participatory Research (CBPR) approach to increase knowledge and improve the health and well-being of the communities involved. Written for students, practitioners, researchers, and community members, the book provides a comprehensive presentation of innovative partnership structures and processes, and covers the broad spectrum of methods needed to conduct CBPR in the widest range of research areas--including social determinants of health, health inequities, health promotion, community interventions, disease management, health services, and environmental health. The contributors examine effective methods used within the context of a CBPR approach including survey questionnaire, in-depth interview, focus group interview, ethnography, exposure assessment, and geographic information system mapping. In addition, each chapter describes a case study of the application of the method using a CBPR approach. The book also contains examples of concrete tools and measurement instruments that may be adapted by others involved in CBPR efforts.
Methods for Community-Based Participatory Research for Health (Second Edition)
by Barbara A. Israel Eugenia Eng Amy J. Schulz Edith A. ParkerThis thoroughly revised and updated second edition of Methods for Community-Based Participatory Research for Health provides a step-by-step approach to the application of participatory approaches to quantitative and qualitative data collection and data analysis. With contributions from a distinguished panel of experts, this important volume shows how researchers, practitioners, and community partners can work together to establish and maintain equitable partnerships using a Community-Based Participatory Research (CBPR) approach to increase knowledge and improve the health and well-being of the communities involved. Written for students, practitioners, researchers, and community members, the book provides a comprehensive presentation of innovative partnership structures and processes, and covers the broad spectrum of methods needed to conduct CBPR in the widest range of research areas—including social determinants of health, health inequities, health promotion, community interventions, disease management, health services, and environmental health. The contributors examine effective methods used within the context of a CBPR approach including survey questionnaire, in-depth interview, focus group interview, ethnography, exposure assessment, and geographic information system mapping. In addition, each chapter describes a case study of the application of the method using a CBPR approach. The book also contains examples of concrete tools and measurement instruments that may be adapted by others involved in CBPR efforts.
Methods for Fragments Screening Using Surface Plasmon Resonance
by Sameer Mahmood Zaheer Ramachandraiah GosuThis volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interaction of proteins using SPR.
Methods for Genetic Risk Assessment
by David BrusickGenetic disease contributes to a major portion of our health care costs. While most of the human genetic burden is transmitted from generation to generation, environmental chemicals capable of reacting with germ cell DNA could produce new mutations, resulting in an even greater genetic liability for the next generation. The potential impact of environmental mutagens on the health and viability of other living things is important to consider as well. Methods for Genetic Risk Assessment features contributions from international experts to provide a comprehensive review of the current status of genetic risk assessment. You'll learn about various methods and strategies for when and how to conduct genetic risk assessments on human populations. You will also learn about the potential effects of environmental genotoxins on nonhuman organisms.
Methods for Imaging Cell Membranes (Series in Cellular and Clinical Imaging)
by Luca Panconi Daniel Nieves Maria Makarova Dylan OwenThis book will serve as an introduction to microscopy and biomedical imaging methods, with a focus on the study of the distributions and dynamics of molecules on the cell surface. It will provide readers with an in-depth understanding of how modern microscopy methodology can be used to understand the organisation of cell membrane systems and how experiments can be designed around these methodologies. There are numerous methods employed to understand cell membrane organisation, but foremost among them are microscopy methods which can map the distributions of molecules on the cell surface and even map the biophysical properties of membranes themselves. Fluorescence microscopy has been especially widely used due to its specificity and relatively noninvasive nature, allowing live-cell imaging. However, the recent advance of super-resolution fluorescence microscopy has broken the previous resolution limit for this type of microscopy, which has been an important advancement in the field. Atomic force microscopy and electron microscopy have also been deployed to learn about membrane organisation and properties. Each chapter in this volume will be themed around measuring a particular property of cell membranes. In each case, the authors examine the range of methodology applicable to the task, comparing the advantages and disadvantages of each one, and will also provide an overview of important discoveries that have been made using the methodology being discussed. The chapters will cover: • Measuring membrane protein distributions using single-molecule localisation microscopy (SMLM) • Measuring membrane protein dynamics and diffusion using fluorescence correla-tion spectroscopy (FCS) • Mapping membrane lipid backing using environmentally sensitive fluorescence probes • Mapping membrane thickness and rigidity using atomic force microscopy • Mapping membrane proteins and the cytoskeleton using electron microscopy This book will be a valuable resource to graduate and upper-level undergraduate students and industry researchers in the fields of cell biology, microbiology, microscopy, and medical imaging.
Methods for Investigation of Amino Acid and Protein Metabolism (Methods In Nutritional Research Ser. #2)
by Antoine E. El-KhouryContaining all the new as well as classical methodologies used in the investigation of amino acid and protein metabolism in human and animal models, this book is needed because of the dramatic increase in research in this field. There is no other book currently on the market that covers these methods of investigation.Methods for Investigation of Amino Acid and Protein Metabolism explores areas such as amino acid transfer across tissue membranes, past and new applications using stable isotopes, protein synthesis in organs and tissues, and more. Because of the importance of research methods in the field of amino acid and protein nutrition and metabolism, this book facilitates the reader's integration of the concepts involved in these investigative research methods and their corollaries.In addition to helping any nutrition investigator design and conduct appropriate research protocols in this area of nutrition, this book assists students who are planning to investigate amino acid and protein metabolism in humans or laboratory animals.
Methods for Neural Ensemble Recordings (Frontiers in Neuroscience)
by Miguel A. L. NicolelisExtensively updated and expanded, this second edition of a bestseller distills the current state-of-the-science and provides the nuts and bolts foundation of the methods involved in this rapidly growing science. With contributions from pioneering researchers, it includes microwire array design for chronic neural recordings, new surgical techniques for chronic implantation, microelectrode microstimulation of brain tissue, multielectrode recordings in the somatosensory system and during learning, as well as recordings from the central gustatory-reward pathways. It explores the use of Brain-Machine Interface to restore neurological function and proposes conceptual and technical approaches to human neural ensemble recordings in the future.
Methods for Novel Psychoactive Substance Analysis (Methods in Pharmacology and Toxicology)
by Marta Concheiro Karl B. ScheidweilerThis collection provides detailed information on current advances in analytical methods and strategies employed for monitoring and discovering a wide range of novel psychoactive substances (NPS) in clinical and forensic laboratories. The main classes of NPS in terms of prevalence include synthetic cannabinoids, synthetic cathinones, synthetic opioids, and designer or synthetic benzodiazepines, and this book explores selecting the appropriate sample matrix and analytical testing approaches for laboratories faced with NPS drug testing, such as in blood, urine, saliva, and hair. Written for the Methods in Pharmacology and Toxicology series, chapters in this volume feature the kind of detailed implementation advice from the experts that leads to successful results in the lab. Authoritative and practical, Methods for Novel Psychoactive Substance Analysis serves as an ideal guide for forensic and clinical toxicologists, pharmacologists and chemists in academic and research settings, as well as for private laboratories seeking to increase our ability to test for these substances. @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536869121 1107305727 33554432 0 415 0;}@font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-469750017 -1073732485 9 0 511 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; mso-pagination:widow-orphan; font-size:12.0pt; mso-bidi-font-size:11.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-ansi-language:EN-US; mso-fareast-language:EN-US;}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; mso-bidi-font-size:11.0pt; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-ansi-language:EN-US; mso-fareast-language:EN-US;}div.WordSection1 {page:WordSection1;}
Methods for Preclinical Research in Addiction (Neuromethods #174)
by María A. AguilarThis volume presents a wide array of animal models that are used in the study of the individual and environmental factors involved in the development of addiction, the consequences of chronic drug exposure, and recently developed behavioral and biological procedures for the treatment of addictive disorders. The chapters in this book are organized into three sections that cover the aforementioned aspects in relation to different drugs of abuse and pathological gambling. The first section of the book focuses on the individual variables that affect vulnerability to addiction to nicotine, alcohol, cocaine, psychostimulants, and gambling. Section Two looks at the environmental variables that contribute to the vulnerability to addiction. Section Three explores the consequences of chronic drug consumption and new approaches to the treatment of addiction. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and comprehensive, Methods for Preclinical Research in Addiction is a valuable resource that helps researchers and scientists understand the neurobiological mechanisms that underlie the influence of individual and environmental variables on the risk for addiction, and assists them in developing new behavioral and pharmacological strategies to prevent and treat addictive disorders.
Methods for Skin Absorption
by Barbara W. Kemppainen William G. ReifenrathThis up-to-date volume describes the wide variety of available methods concerning percutaneous absorption of drugs and toxins. It discusses in detail the advantages and disadvantages of each method. This unique publication provides a clear, systematic presentation of each aspect which must be considered when designing and performing skin penetration experiments and when interpreting results. It includes specific information regarding necessary supplies and equipment, along with commercial sources for these items. This state-of-the-art book is an ideal instructional manual for investigators uninitiated in performing percutaneous absorption and metabolism studies. Those involved with investigative dermatology, dermatological research, toxicology, and pharmacology will find this reference interesting and indispensable.
Methods for Stability Testing of Pharmaceuticals (Methods in Pharmacology and Toxicology)
by Sanjay Bajaj Saranjit SinghThis detailed volume collects numerous methods and protocols related to different aspects of stability programs that are followed in pharmaceutical development laboratories. Implementation of a successful stability program, vital in preventing product failures and recalls, requires critical and logical thinking that goes beyond the regular documented protocols and methods, so the experiences of the book’s internationally-based expert contributors fill the chapters with practical guidance. As a volume in the Methods in Pharmacology and Toxicology series, this book presents the kind of real-world advice that is essential for advancing laboratory research. Authoritative and thorough, Methods for Stability Testing of Pharmaceuticals serves as a valuable addition to the existing armamentarium of resources available to stability testing personnel in research and industry.
Methods for Studying Nucleic Acid/Drug Interactions
by YorkMeni Wanunu•Yitzhak TorSince most therapeutic efforts have been predominantly focused on pharmaceuticals that target proteins, there is an unmet need to develop drugs that intercept cellular pathways that critically involve nucleic acids. Progress in the discovery of nucleic acid binding drugs naturally relies on the availability of analytical methods that assess the eff
Methods for the Discovery and Characterization of G Protein-Coupled Receptors
by Craig W. StevensThe goal of the characterization and discovery of G protein-coupled receptors, arguably the most important class of signaling molecules in humans and other vertebrates, has spawned numerous vital methodologies. In Methods for the Discovery and Characterization of G Protein-Coupled Receptors, experts in the field present the very latest on the methods and technology used to characterize and discover novel mechanisms of GPCRs which, in many cases, can be used directly to design experiments for the reader's particular GPCR of interest and their specific avenue of investigation. Divided into four convenient sections, this detailed volume covers GPCRs in the genome, trafficking of GPCRs, GPCRs on the membrane, as well as the regulation of these key receptors. Chapters also feature an important section called "Future Directions" which gives the reader an insight into advances soon to be realized in each area. Written for the popular Neuromethods series, this book contains the kind of detailed description and implementation advice that is crucial for getting optimal results. Authoritative and cutting-edge, Methods for the Discovery and Characterization of G Protein-Coupled Receptors serves as an ideal guide for scientists determined to further our knowledge of crucially important set of receptors.