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Cryo-Electron Microscopy in Structural Biology: From Structural Insights to Tomography and Drug Discovery
by Krishnarao AppasaniCryo-electron microscopy, in combination with tomography, has emerged as a new technology for visualizing molecular structures at a resolution beyond even 1 Å. Using this technology has revealed the native molecular details of viruses, membranes, enzymes, ribosomes, and cells. This comprehensive volume brings together authoritative overviews of these methods from structural and biological perspectives. It is a must-have for researchers and graduate students, as well as those working in industry, primarily in the areas of biophysics, structural biology, crystallography, and genomics.Key Features• Focuses on the applications of cryo-EM to structural biology• Documents the importance of cryo-EM/ET approaches in studying the structural determinants of cellular organelle and membrane protein biochemistry• Reviews the applications of high-resolution structures of viruses• Emphasizes structural insights of nuclear and gene machineries• Includes a section focused entirely on the applications of cryo-EM/ET in drug discovery and therapeutic development
Cryo-Electron Tomography: Structural Biology in situ (Focus on Structural Biology #11)
by Friedrich Förster Ariane BriegelThis book presents key aspects and recent developments of cryogenic sample electron tomography (cryo-ET) methodology, authored by leading experts in the field. Understanding structure and function of biomolecules in the context of cells is a new frontier in cellular and structural biology. To facilitate such research, cryo-ET is a key method to visualize the molecules of life in their native settings. Cryo-ET enables the imaging of samples that are preserved in a near-native state, at (macro)-molecular resolution and in three dimensions. Thus, this technique is a unique tool to gain insights into how biomolecules collaborate in orchestrating fundamental biological processes, how mutations cause diseases, pathogens cause infections, and to develop novel therapeutics to treat such illnesses. This book provides a unique reference for the emerging field of cryo-ET. The topics covered range from the fundamental principles of imaging to sample preparation, data analysis,and data sharing within the scientific community. It serves as a valuable resource for the next generation of structural biologists, making it suitable both for undergraduate students studying biochemistry, biophysics, and molecular biology and highly valuable for the more experienced and specialized PhD student. Furthermore, it stands as a state-of-the–art source of knowledge for the established senior scientist within the field of structural biology.
Cryobiology for South American Neotropical Fish Species
by Tiantian Zhang Estefania Paredes Danilo P. StreitThe book presents updated information on the cryopreservation of semen, embryos, germ cells and ovarian follicles of neotropical fish from South America. The chapters address the importance of developing germplasm banks of South American fish, both for commercial production and for conservation of ichthyofauna. This book has been designed mainly to provide a historical review of the 40 years of reproductive cryobiology research on South American fish species. The work is aimed at students, researchers and technicians involved in the areas related to the book's theme. The editors invite the readers to delve into dense technical knowledge, developed by renowned researchers.
Cryocoolers: Theory and Applications (International Cryogenics Monograph Series)
by Milind D. AtreyThis book serves as an introduction to cryocooler technology and describes the principle applications of cryocoolers across a broad range of fields. It covers the specific requirements of these applications, and describes how the advantages and disadvantages of different cryocooler systems are taken into consideration. For example, Stirling coolers tend to be used only in space applications because of their high coefficient of performance, low weight and proven reliability, whilst Gifford-McMahon coolers are used for ground applications, such as in cryopumps and MRI shield cooling applications. Joule-Thomson cryocoolers are used in missile technology because of the fast cool down requirements. The cryocooler field is fast developing and the number of applications are growing because of the increasing costs of the cryogens such as Helium and Neon. The first chapter of the book introduces the different types of cryocoolers, their classification, working principles, and their design aspects, and briefly mentions some of the applications of these systems. This introductory chapter is followed by a number of contributions from prominent international researchers, each describing a specific field of application, the cooling requirements and the cryocooler systems employed. These areas of application include gas liquefaction, space technology, medical science, dilution refrigerators, missile systems, and physics research including particle accelerators. Each chapter describes the cooling requirements based on the end use, the approximate cooling load calculations, the criteria for cryocooler selection, the arrangement for cryocooler placement, the connection of the cooler to the object to be cooled, and includes genuine case studies. Intended primarily for researchers working on cryocoolers, the book will also serve as an introduction to cryocooler technology for students, and a useful reference for those using cryocooler systems in any area of application.
Cryogenic Engineering and Technologies: Principles and Applications of Cryogen-Free Systems
by Dr Chao WangCryogen-free cryogenics is leading a revolution in research and industry by its significant advantages over traditional liquid helium systems. This is the first overview for the field, covering the key technologies, conceptual design, fabrication, operation, performance, and applications of these systems. The contents cover important topics such as the operating principles of 4K cryocoolers, enabling technologies (including vibration reduction) for cryogen free systems, the cryogen- free superconducting magnet, and cryogen-free systems that reach mK. It highlights the wide range of applications in materials science, quantum physics, astronomy and space science, medical sciences and etc. Key features: Introduce technologies and practical know-how employed for cryogen-free systems of using 4 K cryocoolers to replace liquid helium; Address state of the arts of cryogen-free superconducting magnets, sub-kelvin refrigeration systems of He-3 sorption cooler, adiabatic demagnetization refrigerator (ADR) and dilution refrigerators (DR). Discuss applications of cryogen-free systems in modern instruments and equipment.
Cryogenic Engineering, Revised and Expanded
by Thomas FlynnWritten by an engineering consultant with over 48 years of experience in the field, this Second Edition provides a reader-friendly and thorough discussion of the fundamental principles and science of cryogenic engineering including the properties of fluids and solids, refrigeration and liquefaction, insulation, instrumentation, natural gas processi
Cryogenic Heat Management: Technology and Applications for Science and Industry
by Jonathan Demko James E. Fesmire Quan-Sheng ShuCryogenic engineering (cryogenics) is the production, preservation, and use or application of cold. This book presents a comprehensive introduction to designing systems to deal with heat – effective management of cold, exploring the directing (or redirecting), promoting, or inhibiting this flow of heat in a practical way. It provides a description of the necessary theory, design methodology, and advanced demonstrations (thermodynamics, heat transfer, thermal insulation, fluid mechanics) for many frequently occurring situations in low-temperature apparatus. This includes systems that are widely used such as superconducting magnets for magnetic resonance imaging (MRI), high-energy physics, fusion, tokamak and free electron laser systems, space launch and exploration, and energy and transportation use of liquid hydrogen, as well as potential future applications of cryo-life sciences and chemical industries. The book is written with the assumption that the reader has an undergraduate understanding of thermodynamics, heat transfer, and fluid mechanics, in addition to the mechanics of materials, material science, and physical chemistry. Cryogenic Heat Management: Technology and Applications for Science and Industry will be a valuable guide for those researching, teaching, or working with low-temperature or cryogenic systems, in addition to postgraduates studying the topic. Key features: Presents simplified but useful and practical equations that can be applied in estimating performance and design of energy-efficient systems in low-temperature systems or cryogenics Contains practical approaches and advanced design materials for insulation, shields/anchors, cryogen vessels/pipes, calorimeters, cryogenic heat switches, cryostats, current leads, and RF couplers Provides a comprehensive introduction to the necessary theory and models needed for solutions to common difficulties and illustrates the engineering examples with more than 300 figures
Cryogenic Heat Transfer (Chemical And Mechanical Engineering Ser.)
by Gregory Nellis Randall BarronCryogenic Heat Transfer, Second Edition continues to address specific heat transfer problems that occur in the cryogenic temperature range where there are distinct differences from conventional heat transfer problems. This updated version examines the use of computer-aided design in cryogenic engineering and emphasizes commonly used computer programs to address modern cryogenic heat transfer problems. It introduces additional topics in cryogenic heat transfer that include latent heat expressions; lumped-capacity transient heat transfer; thermal stresses; Laplace transform solutions; oscillating flow heat transfer, and computer-aided heat exchanger design. It also includes new examples and homework problems throughout the book, and provides ample references for further study. New in the Second Edition: Expands on thermal properties at cryogenic temperatures to include latent heats and superfluid helium Develops the material on conduction heat transfer and divides it into four separate chapters to facilitate understanding of the separate features and computational techniques in conduction heat transfer Introduces EES (Engineering Equation Solver), a computer-aided design tool, and other computer applications such as Maple Describes special features of heat transfer at cryogenic temperatures such as analysis with variable thermal properties, heat transfer in the near-critical region, Kapitza conductance, and network analysis for free-molecular heat transfer Includes design procedures for cryogenic heat exchangers Cryogenic Heat Transfer, Second Edition discusses the unique problems surrounding conduction heat transfer at cryogenic temperatures. This second edition incorporates various computational software methods, and provides expanded and updated topics, concepts, and applications throughout. The book is designed as a textbook for students interested in thermal problems occurring at cryogenic temperatures and also serves as reference on heat transfer material for practicing cryogenic engineers.
Cryogenic Helium Refrigeration for Middle and Large Powers (International Cryogenics Monograph Series)
by Guy Gistau BaguerThis book offers a practical introduction to helium refrigeration engineering, taking a logical and structured approach to the design, building, commissioning, operation and maintenance of refrigeration systems. It begins with a short refresher of cryogenic principles, and a review of the theory of heat exchangers, allowing the reader to understand the importance of the heat exchanger role in the various thermodynamic cycle structures. The cycles are considered from the simplest (Joule Thomson) to the most complicated ones for the very large refrigeration plants and, finally, those operating at temperatures lower than 4.5 K. The focus then turns to the operation, ability and limitations of the main components, including room temperature cycle screw compressors, heat exchangers, cryogenic expansion turbines, cryogenic centrifugal compressors and circulators. The book also describes the basic principles of process control and studies the operating situations of helium plants, with emphasis on high level efficiency. A major issue is helium purity, and the book explains why helium is polluted, how to purify it and then how to check its purity, to ensure that all components are filled with pure helium prior to starting. Although the intention of the book is not to design thermodynamic cycles, it is of interest to a designer or operator of a cryogenic system to perform some simplified calculations to get an idea of how components or systems are behaving. Throughout the book, such calculations are generally performed using Microsoft® Excel and the Gaspak® or Hepak® software.
Cryogenic Recycling and Processing
by Norman. R BratonThe purpose of this book is to promote useful knowledge in the field of cryogenics. To accomplish this, the manuscript presents a composite of the state-of-the-art knowledge, research, and application of cryogenic processes.
Cryogenic Safety: A Guide to Best Practice in the Lab and Workplace (International Cryogenics Monograph Series)
by Thomas J. Peterson J. G. Weisend IIThis book describes the current state of the art in cryogenic safety best practice, helping the reader to work with cryogenic systems and materials safely. It brings together information from previous texts, industrial and laboratory safety polices, and recent research papers. Case studies, example problems, and an extensive list of references are included to add to the utility of the text. It describes the unique safety hazards posed by cryogenics in all its guises, including issues associated with the extreme cold of cryogenics, the flammability of some cryogenic fluids, the displacement of oxygen by inert gases boiling off from cryogenic fluids, and the high pressures that can be formed during the volume expansion that occurs when a cryogenic fluid becomes a room temperature gas. A further chapter considers the challenges arising from the behavior of materials at cryogenic temperatures. Many materials are inappropriate for use in cryogenics and can fail, resulting in hazardous conditions. Despite these hazards, work at cryogenic temperatures can be performed safely. The book also discusses broader safety issues such as hazard analysis, establishment of a safe work culture and lessons learned from cryogenic safety in accelerator labs. This book is designed to be useful to everyone affected by cryogenic hazards regardless of their expertise in cryogenics.
Cryopolitics: Frozen Life in a Melting World (The\mit Press Ser.)
by Emma Kowal Joanna RadinThe social, political, and cultural consequences of attempts to cheat death by freezing life.As the planet warms and the polar ice caps melt, naturally occurring cold is a resource of growing scarcity. At the same time, energy-intensive cooling technologies are widely used as a means of preservation. Technologies of cryopreservation support global food chains, seed and blood banks, reproductive medicine, and even the preservation of cores of glacial ice used to study climate change. In many cases, these practices of freezing life are an attempt to cheat death. Cryopreservation has contributed to the transformation of markets, regimes of governance and ethics, and the very relationship between life and death. In Cryopolitics, experts from anthropology, history of science, environmental humanities, and indigenous studies make clear the political and cultural consequences of extending life and deferring death by technoscientific means.The contributors examine how and why low temperatures have been harnessed to defer individual death through freezing whole human bodies; to defer nonhuman species death by freezing tissue from endangered animals; to defer racial death by preserving biospecimens from indigenous people; and to defer large-scale human death through pandemic preparedness. The cryopolitical lens, emphasizing the roles of temperature and time, provokes new and important questions about living and dying in the twenty-first century.ContributorsWarwick Anderson, Michael Bravo, Jonny Bunning, Matthew Chrulew, Soraya de Chadarevian, Alexander Friedrich, Klaus Hoeyer, Frédéric Keck, Eben Kirksey, Emma Kowal, Joanna Radin, Deborah Bird Rose, Kim TallBear, Charis Thompson, David Turnbull, Thom van Dooren, Rebecca J. H. Woods
Cryopreservation and Freeze-Drying Protocols (Methods in Molecular Biology #2180)
by Willem F. Wolkers Harriëtte OldenhofThis fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and authoritative, Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution.
Cryopreservation and Freeze-Drying Protocols (Methods in Molecular Biology #38)
by Mark R. Mclellan John G. DayThis book provides detailed protocols for all the latest methodologies used to assure the long-term biostorage of a diverse range of biological materials. Developed in expert laboratories, the protocols have been painstakingly perfected over the years to provide time-tested, step-by-step instructions that ensure robust and reproducible results. Each protocol deals with the preservation of an organelle, cell, or tissue type, and is accessible even to the nonspecialist because of its cookbook approach. Novel protocols can be readily developed with the help of the "hands-on" Notes sections. Cryopreservation and Freeze-Drying Protocols is an indispensable reference work for both the individual researcher, and all those who want to establish or improve biostorage systems in their laboratories. Its applications range from microbial culture collections, botanic gardens, and zoos to animal husbandry, aquaculture, medicine, human fertilization, and cell and molecular biology.
Cryopreservation and Freeze-Drying Protocols, 2nd Edition (Methods in Molecular Biology #368)
by John G. Day Glyn StaceyThis widely expanded second edition offers a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. It includes novel approaches and protocols that were not preservable when the first edition was published.<P><P> The book begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures.
Cryopreservation of Fish Gametes
by Judith Betsy Stephen KumarUnderstanding the reproductive physiology and endocrinology of fishes is essential for captive maturation and seed production in the field of aquaculture. Studying the spermatology of fishes is a comparatively new focus in aquaculture, which has emerged as an important area of fish research over the past two decades. In this regard, the cryopreservation of fish gametes is a crucial aspect. Moreover, energetics studies of gametes have become essential, considering the loss of vigour in the spermatozoa after cryopreservation.The latest development in this context is the cryopreservation of spermatogonial stem cell, which is also covered in the book, along with detailed information on embryo cryopreservation in fishes and crustaceans. The role of cryopreservation in conservation programmes is another important aspect, one that will especially interest biologists.This book addresses central issues in fish gamete cryopreservation and breeding, while also reviewing the history of cryopreservation. Its most unique feature is the breadth of its coverage, from basic information on reproduction in fishes, to such advanced topics as embryo cryopreservation. Chiefly intended as a handy troubleshooting guide, the book represents a valuable resource for research students in related fields.
Cryopreservation of Mammalian Gametes and Embryos: Methods and Protocols (Methods in Molecular Biology #1568)
by Ashok Agarwal Alex C. Varghese Zsolt Peter NagyThis volume provides a comprehensive and technical presentation of numerous aspects of reproductive cell tissue cryopreservation, and presents readers with current procedures and detailed discussions of novel techniques and the latest innovations. The chapters in this book are divided into five parts and cover subjects such as: immature oocyte cryopreservation, human sperm vitrification and slow-freezing, directional freezing of ovarian tissue, automated vitrification systems, and detailed protocols on popular and commercially available cryopreservation/vitrification systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Cryopreservation of Mammalian Gametes and Embryos: Methods and Protocols includes chapters written by leading experts in the field and is a valuable resource for anyone interested in the field of cryopreservation.
Cryopreservation of Plant Cells and Organs
by K.K. KarthaThis monograph will put the topic of Cryopreservation of Plant Cells and Organs in perspective. Originally published in 1985.
Cryostat Design: Case Studies, Principles and Engineering (International Cryogenics Monograph Series)
by J. G. Weisend IIThis book enables the reader to learn the fundamental and applied aspects of practical cryostat design by examining previous design choices and resulting cryostat performance. Through a series of extended case studies the book presents an overview of existing cryostat design covering a wide range of cryostat types and applications, including the magnet cryostats that comprise the majority of the Large Hadron Collider at CERN, space-borne cryostats containing sensors operating below 1 K, and large cryogenic liquid storage vessels. It starts with an introductory section on the principles of cryostat design including practical data and equations. This section is followed by a series of case studies on existing cryostats, describing the specific requirements of the cryostat, the challenges involved and the design choices made along with the resulting performance of the cryostat. The cryostat examples used in the studies are chosen to cover a broad range of cryostat applications and the authors of each case are leading experts in the field, most of whom participated in the design of the cryostats being described. The concluding chapter offers an overview of lessons learned and summarises some key hints and tips for practical cryostat design. The book will help the reader to expand their knowledge of many disciplines required for good cryostat design, including the cryogenic properties of materials, heat transfer and thermal insulation, instrumentation, safety, structures and seals.
Crypt: Life, Death and Disease in the Middle Ages and Beyond
by Alice RobertsTHE SUNDAY TIMES NO. 1 BESTSELLERCHOSEN BY WATERSTONES AS ONE OF THEIR BEST HISTORY BOOKS OF 2024 'Compulsive . . . A wonderful display of how modern archaeology can bring hidden histories to life' Daily Telegraph 'Gripping . . . I found it hard to put down' Evening Standard 'Another really good book from archaeologist Alice Roberts . . . Helps you understand the facts on a technical level, but also makes you feel them in your bones' New Scientist 'Robert's reflections on Thomas Becket and Canterbury Cathedral are especially entertaining . . . Fascinating' Spectator The new book by Sunday Times bestselling author of Ancestors and Buried - the final instalment in Professor Alice Roberts' acclaimed trilogy.We can unlock secrets from bones preserved for centuries in tombs, graves and crypts. The history of the Middle Ages is typically the story of the rich and powerful, there&’s barely a written note for most people&’s lives. Archaeology represents another way of interrogating our history. By using cutting-edge science to examine human remains and burials, it is possible to unearth details about how individuals lived and died that give us a new understanding of the past – one that is more intimate and inclusive than ever before. The seven stories in Crypt are not comforting tales. We meet the patients at one of the earliest hospitals in England and the victims of the St Brice&’s Day Massacre. We see a society struggling to make sense of disease, disability and death, as incurable epidemics sweep through medieval Europe. We learn of a protracted battle between Church and State that led to the murder of Thomas Becket and the destruction of the most famous tomb in England. And we come face to face with the archers who went down with Henry VIII&’s favourite ship, the Mary Rose. Beautifully written and expertly researched by Professor Alice Roberts, Crypt is packed with thrilling discoveries that will make you see the history of Britain afresh.
Cryptic Female Choice in Arthropods: Patterns, Mechanisms and Prospects
by Alfredo V. Peretti Anita AisenbergThis timely book revisits cryptic female choice in arthropods, gathering detailed contributions from around the world to address key behavioral, ecological and evolutionary questions. The reader will find a critical summary of major breakthroughs in taxon-oriented chapters that offer many new perspectives and cases to explore and in many cases unpublished data. Many groups of arthropods such as spiders, harvestmen, flies, moths, crickets, earwigs, beetles, eusocial insects, shrimp and crabs are discussed. Sexual selection is currently the focus of numerous and controversial theoretical and experimental studies. Selection in mating and post-mating patterns can be shaped by several different mechanisms, including sperm competition, extreme sexual conflict and cryptic female choice. Discrimination among males during or after copulation is called cryptic female choice because it occurs after intromission, the event that was formerly used as the definitive criterion of male reproductive success and is therefore usually difficult to detect and confirm. Because it sequentially follows intra- and intersexual interactions that occur before copulation, cryptic female choice has the power to alter or negate precopulatory sexual selection. However, though female roles in biasing male paternity after copulation have been proposed for a number of species distributed in many animal groups, cryptic female choice continues to be often underestimated. Furthermore, in recent years the concept of sexual conflict has been frequently misused, linking sexual selection by female choice irrevocably and exclusively with sexually antagonistic co-evolution, without exploring other alternatives. The book offers an essential source of information on how two fields, selective cooperation and individual sex interests, work together in the context of cryptic female choice in nature, using arthropods as model organisms. It is bound to spark valuable discussions among scientists working in evolutionary biology across the world, motivating new generations to unveil the astonishing secrets of sexual biology throughout the animal kingdom.
Cryptic Species: Morphological Stasis, Circumscription, and Hidden Diversity (Systematics Association Special Volume Series)
by Simon J. Mayo Alexandre K. MonroCryptic species are organisms which look identical, but which represent distinct evolutionary lineages. They are an emerging trend in organismal biology across all groups, from flatworms, insects, amphibians, primates, to vascular plants. This book critically evaluates the phenomenon of cryptic species and demonstrates how they can play a valuable role in improving our understanding of evolution, in particular of morphological stasis. It also explores how the recognition of cryptic species is intrinsically linked to the so-called 'species problem', the lack of a unifying species concept in biology, and suggests alternative approaches. Bringing together a range of perspectives from practicing taxonomists, the book presents case studies of cryptic species across a range of animal and plant groups. It will be an invaluable text for all biologists interested in species and their delimitation, definition, and purpose, including undergraduate and graduate students and researchers.
Cryptococcus neoformans: Methods and Protocols (Methods in Molecular Biology #2775)
by Erin E. McClellandThis volume explores the latest developments in the study of Cryptococcus neoformans and its pathology, along with discussion on newly used therapeutics. The chapters in this book cover a wide range of protocols commonly used in Cryptococcus research such as animal models, genetics techniques, virulence factor phenotyping, and microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Cryptococcus neoformans: Methods and Protocols is a valuable resource that will help researchers further enhance their understanding of this pathogenic yeast, and will aid in new discoveries and therapeutics.
Cryptosporidiosis of Man and Animals
by J. P. DubeyThis book attempts to provide a broad coverage of current information needed by public health workers, physicians, veterinarians, parasitologists, technicians, and various biologists who encounter or work with the parasitic disease Cryptosporidium.
Cryptosporidium and Cryptosporidiosis
by Lihua Xiao Ronald FayerFrom the microscopic observation of infection to the widespread application of molecular techniques in taxonomy and epidemiology, to the genome sequencing of two major species and advances in biochemistry, phylogeny, and water treatment, new information on this fascinating genus continues to mount as we discover and utilize the latest scientific te