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Gene Silencing by RNA Interference: Technology and Application
by Muhammad SohailMaximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by r
Gene Synthesis
by Jean PeccoudThe de novo fabrication of custom DNA molecules is a transformative technology that significantly affects the biotechnology industry. Basic genetic engineering techniques for manipulating DNA in vitro opened an incredible field of opportunity in the life sciences. In, Gene Synthesis: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to fabricate DNA . These include methods and techniques for the assembly of oligonucleotide, cloning of synthons into larger fragments, protocols and software applications, and educational and biosecurity impacts of gene synthesis. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Gene Synthese: Methods and Protocols aids scientists in understanding all the different stages of a complex gene synthesis process, while refining their understanding of gene synthesis and determine what part of the process they can or should do in their laboratory and what parts should be contracted to a specialized service provider.
Gene Targeting and Embryonic Stem Cells (Advanced Methods)
by Alison Thomson Jim McwhirGene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference. The recent isolation of human embryonic stem cells and the potential of these cells for therapeutic applications has generated an entirely new methodology. Similarly, gene targeting methodology has recently been extended to nuclear donor cells in ungulate species. This volume will be invaluable for both new and established researchers in the field of human embryonic stem cells, and to biotech companies engaged in the production of transgenic proteins in livestock, xenotransplantation and the development of animal models.
Gene Therapy (Medical Perspectives Ser.)
by Nicholas R. Lemoine David N. CooperGene Therapy describes the delivery systems now available to target a given tissue with specific gene or oligonucleotide sequences, and explores the utility of animal modules as test systems. In the context of selected disease states, it summarises in vitro and in vivo studies and clinical trials performed to date.
Gene Therapy , Third Edition, Volume 2: Design and Characterization of Gene Transfer Vectors
by Joseph LedouxIn Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today’s technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this second volume of Gene Therapy Protocols: Design and Characterization of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the processing and characterization of viral and non-viral gene transfer vectors, as well as promising approaches to design vectors for efficient, targeted and regulated gene delivery and expression. This second volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.
Gene Therapy for Cartilage and Bone Tissue Engineering
by Yu-Chen Hu"Gene Therapy for Cartilage and Bone Tissue Engineering" outlines the tissue engineering and possible applications of gene therapy in the field of biomedical engineering as well as basic principles of gene therapy, vectors and gene delivery, specifically for cartilage and bone engineering. It is intended for tissue engineers, cell therapists, regenerative medicine scientists and engineers, gene therapist and virologists. Dr. Yu-Chen Hu is a Distinguished Professor at the Department of Chemical Engineering, National Tsing Hua University and has received the Outstanding Research Award (National Science Council), Asia Research Award (Society of Chemical Engineers, Japan) and Professor Tsai-Teh Lai Award (Taiwan Institute of Chemical Engineers). He is also a fellow of the American Institute for Medical and Biological Engineering (AIMBE) and a member of the Tissue Engineering International & Regenerative Medicine Society (TERMIS)-Asia Pacific Council.
Gene Therapy for Diseases of the Lung (Lung Biology In Health And Disease Ser.)
by Kenneth L. BrighamThis up-to-the-minute and comprehensive resource lucidly covers gene therapy for lung diseases from existing technologies delivering foreign DNA to the lungs via the airways or circulation to promising new approaches for the further development of safe and efficient gene delivery systems.
Gene Therapy in Reconstructive and Regenerative Surgery
by Giorgio GiatsidisThis book offers an updated overview of the most recent research advances in the field, a comparison of established techniques and methods, a discussion on current experimental and translational challenges, and a commentary on potential opportunities and future directions.Dedicated chapters address and review the preclinical and clinical state-of-the-art of gene therapies for the reconstructive and regenerative surgery of skin and wounds, pathological scars, cartilage, tendons, skeletal muscles, and bio-engineered flaps. A brief guide to developing gene therapy clinical trials in the context of reconstructive and regenerative surgery is also provided.Biomedical and technological innovations are transforming our capacity to use gene therapies to safely and effectively repair, reconstruct, and regenerate tissues that are deficient or have been damaged by trauma and diseases. The targeted and controlled modulation of gene expression in tissues represents a game-changing, next-generation therapeutic tool for the modern reconstructive surgeon, expanding the horizon of regenerative surgery and tissue engineering. Through gene therapies, surgeons can direct (stem) cell differentiation and cell function, modulate the release of growth/transcriptional factors, affect the biological properties of regenerative scaffolds, control tissue inflammation, or induce immune-suppression in composite tissue allotransplants and xenotransplants.Written by renowned reconstructive surgeons and leading experts in each of these fields - from top academic institutions around the globe, the book provides an initial practical guide for veteran and newcomer surgeons alike, as well as for researchers interested in exploring the latest gene-based therapeutic strategies for reconstructive and regenerative surgery.
Gene Transfer and the Ethics of First-in-Human Research
by Jonathan KimmelmanHuman gene transfer is widely regarded as one of the most promising technologies for the treatment of a variety of disorders, but it presents practitioners with a variety of difficult ethical questions. Gene Transfer and the Ethics of First-in-Human Research examines the ethical and policy dimensions of testing interventions in human beings for the first time. The book discusses the difficult ethical challenges that arise from attempting to translate laboratory discoveries into clinical applications. These range from which available techniques to use, when to initiate human testing, questions of consent, expectation in public arenas, how to define acceptable risk, and the inclusion of vulnerable or disadvantaged subjects in early phase trials. This book is relevant to ethicists, legal practitioners, policy makers, geneticists and clinicians involved in clinical trials of new medical interventions.
Gene Transfer to Animal Cells (Advanced Methods)
by Richard TwymanGene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles. Gene Transfer to Animals Cells addresses this problem by describing the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. A final chapter provides an overview of all the applications of animal cell transformation in farming, medicine and research.
Gene Vaccines
by Richard Weiss Sandra Scheiblhofer Josef ThalhamerThe induction of antigen-specific immune responses after in vivo transfection with expression plasmids has triggered a revolution of vaccine research. After a first hype, evoked by the fascinating options of this method, clinical studies did not reach the ambitious aims and a phase of disillusion ensued. It became obvious that Gene vaccines displayed a weaker immunogenicity in humans than had been observed in the mouse models. Meanwhile these hurdles have been overcome and gene vaccines undergo a renaissance. The present book gives an update of the "world of naked gene vaccines", namely DNA and RNA vaccines. Its content ranges from general mechanisms, inherent immunostimulatory properties and the vast potential to modulate immune responses, to recent successful clinical studies and approved veterinary gene vaccines. Beyond the state-of-the-art of genetic immunization, the reader will be stimulated with a chapter addressing "burning questions".
Gene Wars: The Politics of Biotechnology (Open Media Series)
by Kristin DawkinsDespite technological advances, an alarming number of people in the world go hungry. Even more chilling is the fact that in the future that number will likely increase. In this book, Kristin Dawkins discusses the international policies that are shaping this future, including those that govern the genetic engineering of plants. Dawkins shows how a diversified gene pool is crucial to food production - and how corporate control of the gene pool threatens our collective security.Behind these issues lies the specter of globalization - transnational corporations freely exploiting the resources and consumers of the world while political power shifts to remote international institutions strictly dedicated to commerce. Dawkins challenges those in power to develop global systems of political discourse in the public interest and shows how each one of us can make a difference.
Gene and Cell Delivery for Intervertebral Disc Degeneration (Gene and Cell Therapy)
by Raquel Madeira Gonçalves Mario Adolfo BarbosaIntervertebral disc degeneration is one of the major causes of lower back pain for which the common therapeutic interventions are not efficient. A search for alternative therapies for lower back pain and intervertebral disc degeneration includes cell-based therapies. Unfortunately, intervertebral disc degeneration is avascular and thus a hostile environment for cell survival. Furthermore, cellular characterization in intervertebral disc degeneration, and particularly in the nucleus pulposus, is controversial, mainly due to lack of specific markers and species variability. This book adds to the knowledge on cellular and molecular therapies for intervertebral disc degeneration and associated lower back pain. <P><P>Key Selling Features: <li>Describes the ontogeny and phenotype of intervertebral disc cells <li>Reviews the role that inflammation plays in disco-genic pain <li>Highlights the types of cells that might be used as sources for treating degenerating intervertebral discs <li>Summarizes current alternative therapies <li>Explores methods for cell delivery into degenerated intervertebral discs
Gene and Cell Therapies for Beta-Globinopathies
by Punam Malik John TisdaleHemoglobin defects, specifically sickle cell disease & thalassemia, combined, constitute the most common monogenic disorders in the world. In fact, nearly 2% of the world's population carries a globin gene mutation. The transfer of the corrective globin gene through the HSC compartment by allogeneic HSC transplantation (HSCT) has already proven curative in both SCD and thalassemia patients, and provides the proof of concept that genetic manipulation of the defective organ might be equally therapeutic. However, procedural toxicities and the requirement of an HLA-matched sibling donor limit this approach to a fraction of affected individuals. The editors review the progress & the state of the field in HSCT for hemoglobinopathies & shed light on the major changes expected in the next decade. Although allogeneic HSCT is a curative option, it is limited by the availability of matched donors, which are often available only to 15-20% of patients. An alternative to allogeneic HS CT is genetic correction of autologous HSCs, to overcome donor availability & immune side effects. This Book reviews the progress made on additive gene therapy approaches & the current state of the field. Finally, targeted genetic correction is emerging as a novel therapeutic strategy in the hemoglobinopathies. Although ideal, the inefficiency of targeted correction was rate limiting for translation of this technology to the clinic. With advancements in zinc finger nucleases and TALE endonuclease mediated targeted correction, correction frequencies in hematopoietic stem cells is now reaching levels that may become clinically relevant. Furthermore, the ability to generate autologous embryonic stem cell like cells from primary somatic cells (skin fibroblasts or hematopoietic cells) of the affected individual has allowed for the potential application of genetic correction strategies. This Book reviews upcoming genetic strategies to reactivate fetal hemoglobin production and research advances.
Gene and Cell Therapy: Biology and Applications
by Giridhara R. JayandharanRecent advances in stem cell biology, nanotechnology and gene therapy have opened new avenues for therapeutics. The availability of molecular therapeutics that rely on the delivery of DNA, RNA or proteins, harnessing enhanced delivery with nanoparticles, and the regenerative potential of stem cells (adult, embryonic or induced pluripotent stem cells) has had a tremendous impact on translational medicine. The chapters in this book cover a range of strategies for molecular and cellular therapies for human disease, their advantages, and central challenges to their widespread application. Potential solutions to these issues are also discussed in detail. Further, the book addresses numerous advances in the field of molecular therapeutics that will be of interest to the general scientific community. Lastly, the book provides specific examples of disease conditions for which these strategies have been transferred to the clinic. As such, it will be extremely useful for all students, researchers and clinicians working in the field of translational medicine and molecular therapeutics.
Gene and Cell Therapy: Therapeutic Mechanisms and Strategies, Fourth Edition
by Nancy Smyth TempletonThe fourth edition of this bestseller presents extensive background and basic information, state-of-the-art technologies, important achievements, and lingering challenges in the fields of gene and cell therapies. It continues to provide the most comprehensive coverage of these fields in one volume. Some chapters have expanded introductions, making the book even more suitable for classroom use. This edition also offers more material on the contributors' research efforts as well as current technologies, disease targets, and clinical applications.
Gene zum Frühstück: 95 Kolumnen aus der Biologie
by Reinhard Renneberg Iris RapoportDie Autoren entführen uns auch in ihrem neuen Werk in die spannende und vielfältige Welt der Biochemie und Biotechnologie. Das Buch befasst sich mit einer Vielzahl interessanter Fragen und Fakten. Gentechnik – darf man das, soll man das oder muss man das? Die Autoren spüren dem Traum vom Jungbrunnen nach und versuchen Paganinis Geheimnis zu lüften. Sie verblüffen uns, wo alte Handarbeitstechniken plötzlich gefragt sind oder giftige Skorpione sich nützlich machen. Geschrieben in Zeiten, in denen Corona uns beutelt, wurden unsichtbare Feinde, und wie wir uns gegen sie wehren, natürlich nicht ausgespart. Nebenbei wird auch geklärt, was brauner Haut und buntem Obst gemeinsam ist, wie Vitamine in den Kuchen kommen und worin Monets Tragik bestand.
Gene, Drug, and Tissue Engineering (Methods in Molecular Biology #2575)
by Glaucia C. PereiraThis book combines discursive chapters that present the latest progress in molecular biology, drug discovery, organ-tissue engineering, and related fields, with a number of descriptive chapters on methods, protocols, and case studies. Structured into four parts, this volume walks the reader through the latest in cellular biology, with discussions on novel medicinal plant metabolites, nanotechnology in precision medicine, nucleic acid-based therapeutics and vaccines, genetic engineering, computational aid, bioinformatics, synthetic organs for transplantation, and organ-tissue engineering. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice that ensures quality results in the lab. Authoritative and informative, Gene, Drug, and Tissue Engineering serves as an ideal guide for undergraduate students, postgraduate researchers, and senior researchers working in biomedicine and its underlying technologies, stimulating both computational and experimental development and fostering the exchange of new ideas.
Gene-Edited Crops: The CRISPR Solution for Global Food Security
by Aftab AhmadCRISPR-based genome editing has revolutionized the precise genetic improvement of agriculturally important crops, offering unprecedented precision and efficiency in crop improvement. This powerful technology holds immense potential to reshape the future of food security, enabling scientists and agriculturalists to develop resilient, high yield crops resistant to diseases, pests, and environmental stresses. Gene-Edited Crops: The CRISPR Solution for Global Food Security offers a comprehensive guide to the latest research, advancements, and real-world applications of CRISPR in crop genetic improvement for sustainable agriculture and food security.Through in-depth chapters, this book provides an accessible yet rigorous exploration of CRISPR technology, CRISPR reagents and their potential delivery methods, recent developments in CRISPR, and its transformative role in agriculture and food security. With a focus on practical implementation and future potential, this book examines the science behind genome editing and the strategic pathways to address key challenges in crop development—from enhancing nutritional profiles to improving environmental adaptability.Features include: Comprehensive overview: An introduction to the fundamentals of CRISPR technology and its application in crop science. Computational tools and CRISPR reagents: Bioinformatic tools for designing precise gRNA, CRISPR reagents and their effective delivery methods. Recent developments in CRISPR: Emerging developments in CRISPR for precise gene editing in plants such as base editing, prime editing, Fanzor, and multiplex gene editing. Real-world applications: Application of CRISPR for developing future crops, including projects targeting enhanced disease resistance, drought tolerance, and improved nutritional content. Regulatory landscape: Insightful discussion on the evolving global regulatory framework governing gene-edited crops, including the transformative role of artificial intelligence in navigating these regulations. Future directions: Exploration of ongoing advances and the outlook for CRISPR technology in addressing food security. With detailed examples, data, and resources, this book serves as an essential reference for scientists, agriculturalists, and policymakers invested in the future of food security. It also doubles as an educational resource for advanced courses in genetics, plant sciences, and agricultural biotechnology, making it invaluable to both seasoned researchers and those newly interested in the potential of CRISPR.
Gene-Environment Interplay in Interpersonal Relationships across the Lifespan
by Briana N. Horwitz Jenae M. NeiderhiserIntriguing new findings on how genes and environments work together through different stages of life take the spotlight in this significant collection. Studies from infancy to late adulthood show both forces as shaping individuals' relationships within family and non-family contexts, and examine how these relationships, in turn, continue to shape the individual. Transitional periods, in which individuals become more autonomous and relationships and personal identities become more complicated, receive special emphasis. In addition, chapters shed light on the extent to which the quantity and quality of genetic and environmental influence may shift across and even within life stages. Included in the coverage: Gene-environment interplay in parenting young children. The sibling relationship as a source of shared environment. Gene-environment transactions in childhood and adolescent problematic peer relationships. Toward a developmentally sensitive and genetically informed perspective on popularity. Spouse, parent, and co-worker: roles and relationships in adulthood. The family system as a unit of clinical care: the role of genetic systems. Behavioral geneticists, clinical psychologists, and family therapists will find in Gene-Environment Interplay in Interpersonal Relationships across the Lifespan a window into current thinking on the subject, new perspectives for understanding clients and cases, and ideas for further study.
Gene-Mapping Techniques and Applications
by Lawrence B. Schook Harris A. Lewin David G. McLarenThis book explains current strategies for mapping genomes of higher organisms and explores applications of gene mapping to agriculturally important species of plants and animals. It also explores the experimental techniques used for genetic and physical mapping of genes.
GeneRisk
by Broder Breckling Winfried Schröder Gunther SchmidtDas Buch gibt einen Einblick in systemische Verknüpfungen, denen der Anbau von gentechnisch veränderten Pflanzen in Deutschland unterliegen würde. Es thematisiert Verknüpfungen, die sich zwischen ökologischen und sozialen Systemzusammenhängen herstellen lassen. Bisherige Betrachtungen widmeten sich dem Thema entweder aus ökologischer Perspektive oder thematisieren (agrar-)politische Zusammenhänge. Das Buch resultiert aus einem Forschungsprojekt, in welchem das BMBF die Erforschung sozialökologischer Wechselwirkungen gefördert hat.
Genealogy Standards
by Board for Certification of GenealogistsHow do family historians know they are producing or receiving trustworthy results? This official manual from the Board of Certification for Genealogists, essentially a users' guide for family historians, provides standards for genealogical researchers to assess their own and others' work. <p><p>The revised second edition increases the clarity of DNA and privacy standards. Those standards are especially useful in the twenty-first century, when many genealogists use a complex new tool―DNA testing―and trace living people more often than they did in the past.
Genentech: The Beginnings of Biotech
by Sally Smith HughesIn the fall of 1980, Genentech, Inc. , a little-known California genetic engineering company, became the overnight darling of Wall Street, raising over $38 million in its initial public stock offering. Lacking marketed products or substantial profit, the firm nonetheless saw its share price escalate from $35 to $89 in the first few minutes of trading, at that point the largest gain in stock market history. Coming at a time of economic recession and declining technological competitiveness in the United States, the event provoked banner headlines and ignited a period of speculative frenzy over biotechnology as a revolutionary means for creating new and better kinds of pharmaceuticals, untold profit, and a possible solution to national economic malaise. Drawing from an unparalleled collection of interviews with early biotech players, Sally Smith Hughes offers the first book-length history of this pioneering company, depicting Genentech's improbable creation, precarious youth, and ascent to immense prosperity. Hughes provides intimate portraits of the people significant to Genentech's science and business, including cofounders Herbert Boyer and Robert Swanson, and in doing so sheds new light on how personality affects the growth of science. By placing Genentech's founders, followers, opponents, victims, and beneficiaries in context, Hughes also demonstrates how science interacts with commercial and legal interests and university research, and with government regulation, venture capital, and commercial profits. Integrating the scientific, the corporate, the contextual, and the personal, Genentech tells the story of biotechnology as it is not often told, as a risky and improbable entrepreneurial venture that had to overcome a number of powerful forces working against it.
General Agriculture for West Africa (Routledge Library Editions: Agriculture #13)
by Q.B. Olatunji AnthonioFirst published in 1979. This study examines various aspects of agriculture in West Africa. There is a strong chapter on the economics of agriculture and records, farm machinery, agricultural improvement, fish and the basics of crop and livestock production are all dealt with. The role of government policy in the improvement of agriculture is also examined. This title will be useful to undergraduates concerned with agriculture who want a good grounding before going on to their specialised fields of the applied sciences, as well as to those interested in commercial farming and policy makers in civil administration.