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Gene Control
by David LatchmanGene Control offers a current description of how gene expression is controlled in eukaryotes, reviewing and summarizing the extensive primary literature into an easily accessible format. Gene Control is a comprehensively restructured and expanded edition of Latchman’s Gene Regulation: A Eukaryotic Perspective, Fifth Edition. The first part of the book deals with the fundamental processes of gene control at the levels of chromatin structure, transcription, and post-transcriptional processes. Three pairs of chapters deal with each of these aspects, first describing the basic process itself, followed by the manner in which it is involved in controlling gene expression. The second part of the book deals with the role of gene control in specific biological processes. Certain chapters deal with the importance of gene control in cellular signaling processes and for normal development of the embryo. Another chapter discusses the key roles played by gene-regulatory processes in the specification of differentiated cell types such as muscle cells and neurons. The final chapters discuss the consequences of errors in gene control; the relationship between gene misregulation and human diseases, especially cancer; and potential therapies designed specifically to target particular levels of gene control. Gene Control will be of value to students in biological sciences, as well as to scientists and clinicians interested in how genes are regulated in health and disease.
Gene Control
by David LatchmanThe new edition of Gene Control has been updated to include significant advances in the roles of the epigenome and regulatory RNAs in gene regulation. The chapter structure remains the same: the first part consists of pairs of chapters that explain the mechanisms involved and how they regulate gene expression, and the second part deals with specific biological processes (including diseases) and how they are controlled by genes. Coverage of methodology has been strengthened by the inclusion more explanation and diagrams. The significant revision and updating will allow Gene Control to continue to be of value to students, scientists and clinicians interested in the topic of gene control.
Gene Control
by David S. Latchman Venugopalan CheriyathThe new edition of Gene Control, for the first time, provides extensive coverage on prokaryotic gene regulation, which makes it the only textbook offering a complete and detailed account of gene control for both prokaryotic and eukaryotic organisms. The core objective of this edition is to educate students about the fundamental principles and mechanisms governing gene expression, regulation, and function. To reinforce these ideas, each chapter now includes discussion questions to promote critical thinking. There are also multiple choice questions and animations for students, and a large question bank and figure slides for instructors. The textbook also emphasizes the vital role of scientific experiments and evidence in shaping our current understanding of gene control and provides comprehensive coverage of essential gene expression techniques and methodologies throughout the book.This extensively updated edition of the renowned textbook Gene Control will remain a valuable resource for students, instructors, researchers, and medical professionals exploring various aspects of gene control, ranging from the regulation of genes in infectious diseases to embryonic development across different organisms, from bacteria to humans.
Gene Correction
by Francesca StoriciGene correction is a technology that gives us the tools for both repairing and mutating DNA, for discovering gene functions and for engineering new genetic variants. Gene Correction: Methods and Protocols provides a user friendly, detailed and up-to-date collection of strategies and methodologies utilized for generating specific sequence changes in the DNA of cells in the laboratory, while also tackling the major problems that the field of gene correction faces. This volume brings together many experts in the field of gene correction to disclose a wide and varied array of specific gene correction protocols for engineering mutations in DNA, for delivering correcting DNA to target cells, and for improving the accuracy and safety of the gene correction process. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Gene Correction: Methods and Protocols seeks to serve scientists of all backgrounds interested in the area of gene targeting/recombination/therapy.
Gene Delivery (Biomaterial Engineering)
by Huayu Tian Xuesi ChenGene therapy has been regarded as a great potential for specific treatment of gene-related human diseases, such as cancer, genetic and epidemic diseases. Gene therapy refers to the biomedical technology that inserts normal or therapeutic exogenous genes into target cells to repair or replace defective genes in target cells, so as to achieve the purpose of treating diseases. Efficient gene delivery systems are the crucial roles for successful implementation of gene therapy. This book provides a platform for young scholars and students to systematically understand the preparation and characterization of the existing non-viral gene delivery systems, as well as providing a technology platform for clinical gene therapy
Gene Delivery Systems: Development and Applications (ISSN)
by Yashwant V. PathakThis unique volume in our Drugs and Pharmaceutical Sciences series covers the development of gene therapy today, the technology involved, clinical applications of siRNA, non-viral vector-based mRNA delivery using nanotechnology, and RNA based vaccines for treating the infectious diseases. It also presents the current application of the CRISPR/Cas9 gene-editing technique which has revolutionized genome editing and which was awarded the 2020 Nobel Prize in Chemistry. Several new drug delivery systems are explored for the applications of gene therapy. These are found to be useful in treating chronic illnesses, including cancer and infectious diseases. Key Features: Overview of the development of gene therapy Provides the most up to date information on the development of gene therapy, from the technology involved to gene correction and genome editing Presents CRISPR gene therapy recent trends and applications Discusses siRNA, mRNA, and DNA plasmids
Gene Delivery to Mammalian Cells, Volume 1: Nonviral Gene Transfer Techniques
by William C. HeiserHighly experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.
Gene Delivery to Mammalian Cells, Volume 2: Viral Gene Transfer Techniques
by William C. HeiserWhether to assess the function of new genes identified from the Human Genome Project or to apply gene therapy successfully, it is often necessary to deliver genes to specific cells. In Gene Delivery to Mammalian Cells, highly experienced researchers describe in great detail methods that have proven most useful in delivering genes to mammalian cells. Volume 2: Viral Gene Transfer Techniques details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors, adenovirus, adeno-associated viruses, alphavirus, herpes simplex virus, baculovirus, and retrovirus. Many of these techniques have only been in practice for a few years and are still being refined and updated. Some are being used not only in basic science, but also in gene therapy applications. Each protocol contains step-by-step instructions, along with background notes, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Introductory chapters review the delivery methods presented, discussing their advantages and disadvantages, how they have been used successfully for gene delivery, and the future of their technology. An accompanying volume, Volume 1: Nonviral Gene Transfer Techniques, focuses on gene delivery by a variety of chemical and physical methods.<P><P> Comprehensive and cutting-edge, Gene Delivery to Mammalian Cells, volumes 1 and 2, offers biomedical specialists in industry and academia an unrivaled collection of reliable methods for the chemical, physical, and viral delivery of genes to mammalian cells, all prepared to save laboratory time and ensure experimental success.
Gene Delivery: Nanotechnology and Therapeutic Applications (Drugs and the Pharmaceutical Sciences)
by Yashwant PathakGene delivery is a transport of genes of therapeutic values into the chromosomes of the cells or tissues which can be targeted to replace the faulty genes. In last two decades lot of research efforts are dedicated to gene delivery for therapeutic applications. Today gene therapy is promising approach in treatment of genetic diseases including mitochondrial related diseases like blindness, muscular dystrophy, cystic fibrosis, and some cancers. Gene Delivery Systems: Nano Delivery Technologies observes the exploration of nanotechnology for gene therapy and gene delivery. Written by prominent authors in the field, this book covers various aspects of gene delivery including challenges in delivering gene therapy, advances in genome editing, RNA-based gene therapy, Green nanoparticles for oligonucleotide delivery. Additional features include" Provides the most up to date information on the development of gene therapy, from the technology involved to gene correction and genome editing. Includes knowledge of the current application of CRISPR/Cas9 gene-editing technique; an approach that has recently been given the Noble Prize. Examines the development of mRNA vaccines for Covid -19 in challenging pandemic scenario Discusses siRNA, mRNA, and DNA plasmids.
Gene Discovery for Disease Models
by W. GuThis book provides readers with new paradigms on the mutation discovery in the post-genome era. The completion of human and other genome sequencing, along with other new technologies, such as mutation analysis and microarray, has dramatically accelerated the progress in positional cloning of genes from mutated models. In 2002, the Mouse Genome Sequencing Consortium stated that "The availability of an annotated mouse genome sequence now provides the most efficient tool yet in the gene hunter's toolkit. One can move directly from genetic mapping to identification of candidate genes, and the experimental process is reduced to PCR amplification and sequencing of exons and other conserved elements in the candidate interval. With this streamlined protocol, it is anticipated that many decades-old mouse mutants will be understood precisely at the DNA level in the near future. " The implication of such a statement should be similar to the identification of mutated genes from human diseases and animal models, when genome sequencing is completed for them. More than five years have passed, but genes in many human diseases and animal models have not yet been identified. In some cases, the identification of the mutated genes has been a bottleneck, because the genetic mechanism holds the key to understand the basis of the diseases. However, an integrative strategy, which is a combination of genetic mapping, genome resources, bioinformatics tools, and high throughput technologies, has been developed and tested. The classic paradigm of positional cloning has evolved with completely new concepts of genomic cloning and protocols. This book describes new concepts of gene discovery in the post-genome era and the use of streamlined protocols to identify genes of interest. This book helps identify not only large insertions/deletions but also single nucleotide mutations or polymorphisms that regulate quantitative trait loci (QTL).
Gene Drives On The Horizon: Advancing Science, Navigating Uncertainty, And Aligning Research With Public Values
by Committee on Gene Drive Research in Non-Human Organisms Recommendations for Responsible ConductResearch on gene drive systems is rapidly advancing. Many proposed applications of gene drive research aim to solve environmental and public health challenges, including the reduction of poverty and the burden of vector-borne diseases, such as malaria and dengue, which disproportionately impact low and middle income countries. However, due to their intrinsic qualities of rapid spread and irreversibility, gene drive systems raise many questions with respect to their safety relative to public and environmental health. Because gene drive systems are designed to alter the environments we share in ways that will be hard to anticipate and impossible to completely roll back, questions about the ethics surrounding use of this research are complex and will require very careful exploration. Gene Drives on the Horizon outlines the state of knowledge relative to the science, ethics, public engagement, and risk assessment as they pertain to research directions of gene drive systems and governance of the research process. This report offers principles for responsible practices of gene drive research and related applications for use by investigators, their institutions, the research funders, and regulators.
Gene Drives at Tipping Points: Precautionary Technology Assessment and Governance of New Approaches to Genetically Modify Animal and Plant Populations
by Arnim Von Gleich Winfried SchröderThis open access book reports on a pilot project aiming at collecting information on the socio-ecological risks that could arise in the event of an uncontrolled spread of genetically engineered organisms into the environment. The researchers will, for instance, be taking a closer look at genetically engineered oilseed rape, genetically engineered olive flies as well as plants and animals with so-called gene drives. The book mainly adresses researchers.
Gene Drives on the Horizon: Advancing Science, Navigating Uncertainty, and Aligning Research with Public Values
by Engineering Medicine National Academies of SciencesResearch on gene drive systems is rapidly advancing. Many proposed applications of gene drive research aim to solve environmental and public health challenges, including the reduction of poverty and the burden of vector-borne diseases, such as malaria and dengue, which disproportionately impact low and middle income countries. However, due to their intrinsic qualities of rapid spread and irreversibility, gene drive systems raise many questions with respect to their safety relative to public and environmental health. Because gene drive systems are designed to alter the environments we share in ways that will be hard to anticipate and impossible to completely roll back, questions about the ethics surrounding use of this research are complex and will require very careful exploration. Gene Drives on the Horizon outlines the state of knowledge relative to the science, ethics, public engagement, and risk assessment as they pertain to research directions of gene drive systems and governance of the research process. This report offers principles for responsible practices of gene drive research and related applications for use by investigators, their institutions, the research funders, and regulators.
Gene Editing by CRISPR-Cas
by Aarif Ali Farheen ShafiqueCRISPR-Cas systems have redefined the landscape of genetic engineering, offering an unprecedented level of precision, efficiency, and versatility in genome editing. This book, titled Gene Editing by CRISPR-Cas, provides a comprehensive and well-structured approach on the historical context of genetics and gene-editing techniques leading to the groundbreaking discovery of CRISPR-Cas systems.Distinguished by its multidisciplinary scope, this volume not only delves into CRISPR-Cas systems but also examines alternative and next-generation gene-editing technologies. The book aims to inform and engage both general audience with a keen interest in science and biology, and professionals and students in related fields. By offering a balanced blend of scientific explanations and real-world applications, the book seeks to empower readers to understand the potential of CRISPR technology and its impact across diverse domains, such as medicine, agriculture, biotechnology, and diagnostics, while addressing the ethical, regulatory, and biosafety considerations essential for responsible implementation. Furthermore, this book engages in a discussion on the future directions and broader impacts of CRISPR technologies, providing valuable insights into the evolving landscape of genome engineering and its implications for scientific advancements.Key Features:• This book is written in a clear and accessible manner, making complex scientific concepts understandable to a general audience without overwhelming them with technical jargon.• This book provides a comprehensive exploration of the discovery and evolution of CRISPR technology, delving into the underlying science and its transformative capabilities. It serves as an excellent introduction to both the technical intricacies and the human implications of this groundbreaking technology.• This book provides an in-depth exploration of the potential uses of CRISPR across multiple domains, with a particular focus on genetic disease treatment, cancer studies, agricultural advancements, HIV, blood disorders, neurological conditions, insect biology, microbial engineering, and both infectious and non-infectious diseases.• Furthermore, this book also provides critical analysis of bioethics, safety concerns, and regulatory frameworks, providing a responsible perspective on genome editing.
Gene Editing in Plants: CRISPR-Cas and Its Applications
by Ashwani Kumar Krishnendu Mukherjee Shinjiro Ogita Yuan-Yeu Yau Sudipti AroraThis book is a collection of information about applying CRISPR-Cas systems for genome editing in plants. The main focus of this book is to address the recent advances and future prospects of CRISPR-Cas technology in crops.Genome editing technology is important because it can be used to improve plant traits. The earlier genome-engineering tools, zinc finger nucleases (ZFNs), and TAL effector nucleases (TALENs) are complicated to design and not flexible. The novel genome editor, CRISPR-Cas systems, has advantages over ZFNs and TALENs. The advantages are simple and easy to design precision in targeting and efficiency. Due to its precision and simplicity, the CRISPR-Cas technology has rapidly become the most popular genome-editing platform in life-science fields. CRISPR-Cas technology has been used widely for human gene therapy to treat diseases and for plant breeding programs for crop improvement.This book is of interest and useful to genome-editing professionals, plant breeders, horticulturists, field-level extension workers, nurserymen, planters, ecologists, and valuable source of reference to the relevant researchers.
Gene Editing, Law, and the Environment: Life Beyond the Human (Law, Science and Society)
by Irus BravermanTechnologies like CRISPR and gene drives are ushering in a new era of genetic engineering, wherein the technical means to modify DNA are cheaper, faster, more accurate, more widely accessible, and with more far-reaching effects than ever before. These cutting-edge technologies raise legal, ethical, cultural, and ecological questions that are so broad and consequential for both human and other-than-human life that they can be difficult to grasp. What is clear, however, is that the power to directly alter not just a singular form of life but also the genetics of entire species and thus the composition of ecosystems is currently both inadequately regulated and undertheorized. In Gene Editing, Law, and the Environment, distinguished scholars from law, the life sciences, philosophy, environmental studies, science and technology studies, animal health, and religious studies examine what is at stake with these new biotechnologies for life and law, both human and beyond.
Gene Essentiality
by Long Jason LuThis volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and corrections of transposon mutagenesis data, as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Gene Essentiality: Methods and Protocols will aid researchers who wish to further our knowledge in this vital field of study.
Gene Expression Analysis: Methods And Protocols (Methods in Molecular Biology #1783)
by Natàlia Garcia-Reyero Nalini RaghavachariThis volume provides experimental and bioinformatics approaches related to different aspects of gene expression analysis. Divided in three sections chapters detail wet-lab protocols, bioinformatics approaches, single-cell gene expression, highly multiplexed amplicon sequencing, multi-omics techniques, and targeted sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Gene Expression Analysis: Methods and Protocols aims provide useful information to researchers worldwide.
Gene Expression Analysis: Methods and Protocols (Methods in Molecular Biology #2880)
by Nalini Raghavachari Natalia Garcia-ReyeroThis second edition volume expands on the previous edition with updates on the latest methodologies in the transcriptomics field. The chapters in this book cover topics such as spatial omics, long-read sequencing technology, tissue microarrays, analysis of saliva and extracellular vesicles, machine learning and artificial intelligence-based approaches for analysis of singe cells transcriptome, and large sets of data on multi-omics including transcriptomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Gene Expression Analysis: Methods and Protocols, Second Edition is a valuable resource for advanced undergraduate and graduate students studying gene expression analysis, and scientists interested in learning more about this rapidly advancing field.
Gene Expression Profiling
by Richard A. ShimketsLeading scientists in gene expression methodology and bioinformatics data analysis describe readily reproducible methods for measuring RNA levels in cells and tissues. The techniques presented include new methods for applying the Affymetrix GeneChip®, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay®, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. By distilling the basic underlying principles of many methods to a few straightforward concepts, investigators can easily choose the method most appropriate to their application.
Gene Expression Studies Using Affymetrix Microarrays (Chapman & Hall/CRC Computational Biology Series)
by Hinrich Gohlmann Willem TalloenThe Affymetrix GeneChip system is one of the most widely adapted microarray platforms. However, due to the overwhelming amount of information available, many Affymetrix users tend to stick to the default analysis settings and may end up drawing sub-optimal conclusions. Written by a molecular biologist and a biostatistician with a combined decade of
Gene Expression Systems in Fungi: Advancements and Applications
by Monika Schmoll Christoph DattenböckBiotechnology has emerged as one of the key environmentally safe technologies for the future which enables use of biomass to develop novel smart materials and to replace oil derived products. Fungi are the most efficient producers of the enzymes needed for this purpose and in addition they produce a plethora of secondary metabolites, among which novel antibiotics can be found. Industrial application and exploitation of the metabolic capacities of fungi requires highly productive and robust gene expression systems, which can be achieved by selection of appropriate species and strain improvement. In this book we aim to summarize homologous and heterologous gene expression systems of fungi for production of enzymes and secondary metabolites. A broad overview on requirements, challenges and successful applications shall serve as a basis for further development of fungi as biotechnological workhorses in research and industry.
Gene Expression and Its Discontents
by Rodrick Wallace Deborah WallaceThis book describes how epigenetic context, in a large sense, affects gene expression and the development of an organism, using the asymptotic limit theorems of information theory to construct statistical models useful in data analysis. The approach allows deep understanding of how embedding context affects development. We find that epigenetic information sources act as tunable catalysts, directing ontogeny into characteristic pathways, a perspective having important implications for epigenetic epidemiology. In sum, environmental stressors can induce a broad spectrum of developmental dysfunctions, and the book explores a number of pandemic chronic diseases, using U. S. data at different scales and levels of organization. In particular, we find the legacy of slavery has been grossly compounded by accelerating industrial decline and urban decay. Individual chapters are dedicated to obesity and its sequelae, coronary heart disease, cancer, mental disorders, autoimmune dysfunction, Alzheimer's disease, and other conditions. Developmental disorders are driven by environmental factors channeled by historical trajectory and are unlikely to respond to medical interventions at the population level in the face of persistent individual and community stress. Drugs powerful enough to affect deleterious epigenetic programming will likely have side effects leading to shortened lifespan. Addressing chronic conditions and developmental disorders requires significant large-scale changes in public policy and resource allocation.
Gene Expression and its Regulation: An Evolutionary Perspective
by Siddhartha RoyThis textbook for graduate students provides a conceptual framework for comprehending diversity and evolutionary relationships of gene regulatory systems. It elucidates the importance and significance of the physical and chemical principles that underpin gene regulatory processes. The introductory section presents the core principles of the organization of genes, genomes, and aspects of gene regulation in three domains of life. The subsequent narrative then describes the process of gene expression as it goes from the DNA sequence embedded in the genome to the final functional protein. The book then describes how genes are regulated in different domains of life, the principal focus of the book, with considerable mechanistic details. This section also discusses the evolutionary relationships of gene regulatory systems in three domains of life: archaea, eubacteria, and eukarya. In the final section, the book underscores the potential to artificially intervene and alter gene regulation and describes attempts to re-engineer gene regulatory circuits.
Gene Flow: Monitoring, Modeling and Mitigation
by Wei Wei And C. Neal Stewart JrGene flow is a natural process that occurs spontaneously and enables the evolution of life. However, with the release of genetically modified organisms, concerns have focused on introduced foreign transgenes and their dispersal in nature through gene flow. This book examines gene flow of transgenes, such as herbicide resistance genes, with the goal of understanding the factors that may affect the process of gene flow. A greater biological understanding is essential to make sound management regulatory decisions when also taking into consideration the processes that happen in conventional plants. Monitoring, modelling, and mitigation are the three most closely related elements of gene flow. The book includes both scientific reviews and perspectives on gene flow and experimental case studies, including studies of gene flow in soybean and poplar. The authors present diverse views and research methodologies to understand transgene flow. This book: Focuses on applications of gene flow (monitoring, modelling, and mitigation); Includes both review chapters and case studies; Is written by international team of scientists currently working in gene flow. This book will be valuable for students and researchers in genetics, biotechnology, plant science, and environmental science. It also provides key insights of value to regulators of biotechnology as well as policy-makers.