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Geothermal, Wind and Solar Energy Applications in Agriculture and Aquaculture (Sustainable Energy Developments)
by Guangnan Chen Jochen Bundschuh D. Chandrasekharam Janusz PiechockiThe agri-food chain consumes about one third of the world’s energy production with about 12% of it for crop production and nearly 80% for processing, distribution, retail, preparation and cooking. The agri-food chain also accounts for 80-90% of total global freshwater use where 70% alone is for irrigation. Additionally, on a global scale, freshwater production consumes nearly 15% of the entire energy production. It can therefore be argued that making agriculture and the agri-food supply chain independent from fossil fuel use has a huge potential to contribute to global food security and climate protection not only for the next decades but also for the coming century. Provision of secure, accessible and environmentally sustainable supplies of water, energy and food must thus be a priority. One of the major objectives of the world’s scientists, farmers, decisions makers and industrialists is to overcome the present dependence on fossil fuels in the agro-food sector. <P><P>This dependency increases the volatility of food prices and affects economic access to sustenance. This book provides a critical review of recent developments in solar, wind and geothermal energy applications in agriculture and the agro-food sector such as processing, distribution, retail, preparation and cooking.
Geothermie
by Kurt Bucher Ingrid StoberDas Buch bietet neben einer Einführung in die Grundlagen der Geothermie und ihre wichtigsten thermischen und hydraulischen Parameter einen Überblick über alle derzeit verfügbaren geothermischen Nutzungssysteme. Dabei behandeln die Autoren nicht nur Fragen der Effizienz und potenzieller Umweltauswirkungen, sie besprechen auch detailliert die Planung, den Bau und Betrieb von Erdwärmesonden, von hydrothermalen Anlagen und von EGS-Systemen. Themen wie der Hydraulik oder der Hydrochemie, d. h. dem Bereich Lösung und Fällung, sind eigene Kapitel gewidmet.
Geothermie
by Kurt Bucher Ingrid StoberNach einer Einführung in die Grundlagen der Geothermie werden die wichtigsten thermischen und hydraulischen Parameter in ihrer Bedeutung behandelt. Das nunmehr bereits in der 3., überarbeiteten und aktualisierten Auflage vorliegende Buch gibt einen Überblick über alle derzeit verfügbaren Nutzungssysteme der oberflächennahen und tiefen Geothermie und betrachtet diese bezüglich ihrer Effizienz und potentieller Umweltauswirkungen. Neu aufgenommen in die 3. Auflage wurden geothermische Nutzungen in Hochenthalpie-Gebieten mit Beispielen aus Island, USA und Neuseeland. Im Detail besprochen werden Planung, Bau und Betrieb von Erdwärmesonden, von hydrothermalen Anlagen, von EGS-Systemen und von Hochenthalpie-Nutzungen. Spezielle Kapitel sind der Hydraulik, d.h. der Durchführung und Auswertung hydraulischer Tests, der Hydrochemie, d.h. dem Themenbereich Lösung und Fällung, den geophysikalischen Untersuchungsmethoden an der Erdoberfläche und im Bohrloch sowie dem Abteufen und Ausbau von Tiefbohrungen gewidmet. Mit diesem Buch wird erstmalig die Geothermie in ihrer vollen Breite abgedeckt.
Geothermie
by Kurt Bucher Ingrid StoberNach einer Einführung in die Grundlagen der Geothermie werden die wichtigsten thermischen und hydraulischen Parameter in ihrer Bedeutung behandelt. Das nunmehr bereits in der 2., überarbeiteten und aktualisierten Auflage vorliegende Buch gibt einen Überblick über alle derzeit verfügbaren Nutzungssysteme der oberflächennahen und tiefen Geothermie und betrachtet diese bezüglich ihrer Effizienz und potentieller Umweltauswirkungen. Im Detail besprochen werden Planung, Bau und Betrieb von Erdwärmesonden, von hydrothermalen Anlagen und von EGS-Systemen. Spezielle Kapitel sind der Hydraulik, d.h. der Durchführung und Auswertung hydraulischer Tests, und der Hydrochemie, d.h. dem Themenbereich Lösung und Fällung, gewidmet. Mit diesem Buch wird erstmalig die Geothermie in ihrer vollen Breite abgedeckt.
Geotourism Potential of Georgia, the Caucasus: History, Culture, Geology, Geotourist Routes and Geoparks (Geoheritage, Geoparks and Geotourism)
by Irakli Gamkrelidze Avtandil Okrostsvaridze Kakhaber Koiava Ferando MaisadzeGeorgia’s territory represents a real “natural geological laboratory,” exposing magmatic, metamorphic and sedimentary rocks, ranging from the Neoproterozoic to the Quaternary. After a brief presentation of Georgia’s history and culture, the authors present the stratigraphy, rock types of individual tectonic zones of Georgia, their tectonic structure and paleotectonic reconstructions of the Caucasus.This book describes the three main geotourism routes of Georgia meticulously: 1. Tbilisi-Pasanauri-Kazbegi (along the Georgian Military Road), 2. Tbilisi-Zugdidi-Mestia-Ushguli and 3. Tbilisi-Khashuri-Vardzia, which cross different parts of Fold Systems of the Greater and Lesser Caucasus and Transcaucasian Intermountain area.The following potential geoparks are described in this book: 1. Sataplia dinosaur footprints together with Sataplia and Prometheus caves; 2. Tskaltubo resort-town and mineral water deposit; 3. Borjomi resort-town and mineral water deposit; 4. Kazbegi Quaternary volcanoes and Keli volcanic highland;5. Dariali Paleozoic granitoid massif; 6. Dmanisi hominids site and the Mashavera gorge basaltic flow; 7. Dashbashi canyon; 8.Uplistsikhe rock-cut town and Kvakhvreli cave complex; 9.Udabno - Upper Miocene marine and continental deposits and David Gareja monastery complex;10. Dedoplistskaro - Vashlovani protected areas and mud volcanoes.
Geranium and Pelargonium: History of Nomenclature, Usage and Cultivation
by Thomas A. JohnsonConfusion about the genera Geranium and Pelargonium existed even before Linnaeus' binomial system of classification bundled both into the former category in 1753. Despite later evaluations that separated the two, many practitioners of alternative medicine and aromatherapists, among others, remain unaware of the distinction. Laymen and plant sales personnel are often equally in the dark, as the majority of garden center 'geraniums' are Pelargonium species and cultivars. This work aims to dispel myths and peel away layers of incorrect and muddled information which contribute to the unclear image of the genera. Geranium and Pelargonium: The Genus Geranium and Pelargonium features discussion and new information on some of the following areas:* taxonomy and history of usage and nomenclature* cultivation for essential oil production and retail purposes in different countries* phytochemistry of the genera* chemistry of Geranium and Pelargonium oils* theory and practice of distillation of Geranium and Pelargonium oils* pharmacology an therapeutic properties of Geranium and Pelargonium oils and extracts* use of Geranium and Pelargonium products in aromatherapy, perfumery, cosmetics, food processing and medicine* new research into Geranium and PelargoniumThis book will be of interest to graduate students, scientists and professionals in the Geranium and Pelargonium growing and retail industry, the perfumery, food and cosmetics industries, and to those interested in Geranium and Pelargonium for alternative and conventional medical use. * Theory and practice of distillation
Geriatric Imaging
by Ali Guermazi Giuseppe Guglielmi Wilfred C. PehIn the elderly, the coexistence of various diseases, the presence of involutional and degenerative changes, and the occurrence of both physical and cognitive problems represent "the norm." It is therefore important to know how to distinguish the healthy elderly from those in need of treatment as a sound basis for avoiding overdiagnosis and overtreatment. This aspect is a central theme in Geriatric Imaging, which covers a wide range of applications of different imaging techniques and clearly explains both the potential and the limitations of diagnostic imaging in geriatric patients. Individual sections are devoted to each major region or system of the body, and a concluding section focuses specifically on interventional procedures. The book, written by recognized experts in the field, is superbly illustrated and will be an ideal resource for geriatricians, radiologists, and trainees.
Geriatric Neuropsychology: Practice Essentials (Studies on Neuropsychology, Neurology and Cognition)
by Shane S. Bush Thomas A. MartinThe text provides a lifespan developmental approach to neuropsychology. It addresses the many issues in neuropsychological assessment that differ between younger and older adults. It describes the symptoms, neuropathology, diagnostic considerations, and treatment options of common neurological disorders associated with aging. It also addresses special considerations related to geriatric neuropsychology, such as ethical issues, family systems issues, decision-making capacity, cultural consideration, and medical/medication/substance use issues. Additionally, a list of resources for the elderly and their families is also provided.
Geriatric Rehabilitation: From Bedside to Curbside (Rehabilitation Science in Practice Series)
by K. Rao PoduriGeriatric Rehabilitation addresses the fact that this is an age in which individuals have increasing longevity, better health care, education and expectations of health care which present new, increasing and even radical challenges to health care providers. The care of our older patients in rehabilitation settings demands the broad understanding of the key differences in strategies to care for older adults. The combined skills embraced in rehabilitation and geriatrics are presenting unprecedented opportunities for both fields to make substantive and even ground-breaking improvements in the lives of millions of older adults who entrust their lives to us. Rarely in one’s medical career are such opportunities so evident and achievable. Geriatric Rehabilitation edited by Dr. K. Rao Poduri, MD. FAAPMR draws on a distinguished group of authors who are the front-line providers of care to the older adults. This book presents the full spectrum of the unique care needs of older patients who need the combined skills of physical medicine and geriatrics. It provides an easily accessible means of acquiring and improving these new skills for all those involved in geriatric care.
Germ Cell Development in C. elegans
by Tim SchedlGerm cells in sexually reproducing metazoa, through the germline lineage, are the route by which genetic material and cytoplasmic constituents are passed from one generation to the next in the continuum of life. Chapters in this book review germ cell development in the model organism Caenorhabditis elegans, discussing the biology, the genetics and the molecular mechanisms for various processes, as well as drawing comparisons with other organisms. Processes discussed include specification of germ cell fate, meiosis, gametogenesis, environmental/ physiological controls, epigenetics and translational control, fertilization and the oocyte-to-embryo transition. This book thus provides a comprehensive picture of the germline lineage and the continuum of life for the worm.
Germ Cell Development: Methods and Protocols (Methods in Molecular Biology #2770)
by Marco Barchi Massimo De FeliciThis detailed volume explores techniques for the isolation, purification, and establishment of in vitro germ cell (GS) systems at different stages of development in a variety of organisms, including chickens, mice, rats, and humans. The book additionally describes cutting-edge analytic and informatic tools to study germ cell development at the single cell level and meiotic recombination. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Germ Cell Development: Methods and Protocols provides new insight into the understanding of basic biological aspects of germ cell biology, as well as the opportunity for in vitro manipulation of germ cells for toxicology studies and for genetic intervention.
Germ Cell Protocols, Volume 1: Sperm and Oocyte Analysis
by Heide SchattenGerm cell research has produced enormous advances in recent years and more recently has entered into an explosive phase of new discoveries with the introduction of transgenic technologies and nuclear cloning. In Germ Cell Protocols, expert laboratory investigators describe in step-by-step detail a series of powerful techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Chosen for their proven success in advancing our understanding of germ cells, these methods cover sperm and egg activation, motility, fertilization, nuclear development, nuclear cloning, the molecular characterization of specific events, and the imaging of cell structures. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Highlights include in vitro maturation and fertilization of human, porcine, and canine oocytes; the cryopreservation of sperm cells; establishment of an in vitro spermatogenesis system; visualization of sperm accessory structures; and motility assays of spermatozoa. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. An accompanying second volume, Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning, contains methods for molecular egg analysis, live egg imaging, nuclear cloning, oocyte preservation, and nuclear transfer. <P><P> Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-find methods for high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems.
Germ Cell Protocols,Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning
by Heide SchattenGerm cell research has produced enormous advances in recent years and more recently has entered into an explosive phase of new discoveries with the introduction of transgenic technologies and nuclear cloning. In Germ Cell Protocols, expert laboratory investigators describe in step-by-step detail a series of powerful techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Chosen for their proven success in advancing our understanding of germ cells, these methods cover sperm and egg activation, motility, fertilization, nuclear development, nuclear cloning, the molecular characterization of specific events, the imaging of cell structures, and the cryopreservation of germ cells. Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning focuses on molecular egg analysis, live egg imaging, nuclear cloning, oocyte cryopreservation, and nuclear transfer. Highlights include the identification and characterization of Oct-4, germline chromatin silencing by RNAi, cDNA subtraction and cloning in the field of trophoblast/placental development, and selective ablation. There are also methods for PCR-based cloning, differentiating embryonic stem cells into embryonic bodies, live imaging of mammalian embryos using multiphoton microscopy, and the cloning of pig, cattle, rabbit, and mouse oocytes. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. An accompanying earlier volume, Sperm and Oocyte Analysis, focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques.<P><P> Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-find methods for high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems.
Germ Hunter: A Story about Louis Pasteur
by Elaine Marie AlphinGrowing up in the 1830s, Louis Pasteur saw the horrifying effects of diseases like rabies and tuberculosis. Filled with curiosity and imagination, Pasteur began a lifelong search for answers to his many questions about diseases. Although many scientists disagreed with his unusual ideas, his discoveries made him famous. Through his dedication and insight, Pasteur saved millions of lives and laid the groundwork for future medical advancements.
Germ Theory: Medical Pioneers in Infectious Diseases (ASM Books)
by Robert P. GaynesGerm Theory: Medical Pioneers in Infectious Diseases,SECOND EDITION From the ancient worlds of Hippocrates and Avicenna to the early 20th century hospitals of Paul Ehrlich and Lillian Wald to the modern-day laboratories of François Barré-Sinoussi and Barry Marshall, Germ Theory brings to life the inspiring stories of medical pioneers whose work helped change the very fabric of our understanding of how we think about and treat infectious diseases. In beautifully crafted narratives, author Robert Gaynes describes and presents compelling stories, including How Edward Jenner, the pioneer of vaccination, faced down scores of naysayers How a chance discovery led Louis Pasteur to the idea that the virulence of microbes can be altered How scientists in France, the Netherlands, and Denmark produced penicillin during World War 2 while keeping their efforts hidden from the Nazis The second edition features three new chapters based on interviews with Nobel Laureates François Barré-Sinoussi and Barry Marshall, and former NIAID Director and medical advisor to seven U.S. presidents Anthony Fauci, detailing fascinating accounts from their careers, including their groundbreaking work in the areas of HIV, peptic ulcers, and COVID-19, respectively. Winner of a CHOICE Outstanding Academic Title award for its first edition, Germ Theory is required reading for anyone training or working in the field of infectious diseases or with an interest in microbes, the history of medicine, or how new discoveries can bring about paradigmatic shifts in thinking.
Germ Wars: The Politics of Microbes and America's Landscape of Fear
by Melanie ArmstrongThe United States government has spent billions of dollars to prepare the nation for bioterrorism despite the extremely rare occurrence of biological attacks in modern American history. Germ Wars argues that bioterrorism has emerged as a prominent fear in the modern age, arising with the production of new forms of microbial nature and the changing practices of warfare. In the last century, revolutions in biological science have made visible a vast microscopic world, and in this same era we have watched the rise of a global war on terror. Germ Wars demonstrates that these movements did not occur separately but are instead deeply entwined—new scientific knowledge of microbes makes possible new mechanisms of war. Whether to eliminate disease or create weapons, the work to harness and control germs and the history of these endeavors provide an important opportunity for investigating how biological natures shape modern life. Germ Wars aims to convince students and scholars as well as policymakers and activists that the ways in which bioterrorism has been produced have consequences for how people live in this world of unspecifiable risks.
German Political Thought and the Discourse of Platonism: Finding the Way Out of the Cave
by Paul BishopTaking Plato’s allegory of the cave as its starting-point, this book demonstrates how later European thinkers can be read as a reaction and a response to key aspects of this allegory and its discourse of enchainment and liberation. Focusing on key thinkers in the tradition of European (and specifically German) political thought including Kant, Marx, Hegel, Nietzsche, Heidegger, and the Frankfurt School, it relates them back to such foundational figures as Rousseau, Aristotle, and in particular Plato. All these thinkers are considered in relation to key passages from their major works, accompanied by an explanatory commentary which seeks to follow a conceptual and imagistic thread through the labyrinth of these complex, yet fascinating, texts. This book will appeal in particular to scholars of political theory, philosophy, and German language and culture.
German Rocketeers in the Heart of Dixie
by Dr Monique LaneyThis thought-provoking study by historian Monique Laney focuses on the U. S. government-assisted integration of German rocket specialists and their families into a small southern community soon after World War II. In 1950, Wernher von Braun and his team of rocket experts relocated to Huntsville, Alabama, a town that would celebrate the team, despite their essential role in the recent Nazi war effort, for their contributions to the U. S. Army missile program and later to NASA's space program. Based on oral histories, provided by members of the African American and Jewish communities, and by the rocketeers' families, co-workers, friends, and neighbors, Laney's book demonstrates how the histories of German Nazism and Jim Crow in the American South intertwine in narratives about the past. This is a critical reassessment of a singular time that links the Cold War, the Space Race, and the Civil Rights era while addressing important issues of transnational science and technology, and asking Americans to consider their country's own history of racism when reflecting on the Nazi past.
Germany's Ancient Pasts: Archaeology and Historical Interpretation since 1700
by Brent ManerIn Germany, Nazi ideology casts a long shadow over the history of archaeological interpretation. Propaganda, school curricula, and academic publications under the regime drew spurious conclusions from archaeological evidence to glorify the Germanic past and proclaim chauvinistic notions of cultural and racial superiority. But was this powerful and violent version of the distant past a nationalist invention or a direct outcome of earlier archaeological practices? By exploring the myriad pathways along which people became familiar with archaeology and the ancient past—from exhibits at local and regional museums to the plotlines of popular historical novels—this broad cultural history shows that the use of archaeology for nationalistic pursuits was far from preordained. In Germany’s Ancient Pasts, Brent Maner offers a vivid portrait of the development of antiquarianism and archaeology, the interaction between regional and national history, and scholarly debates about the use of ancient objects to answer questions of race, ethnicity, and national belonging. While excavations in central Europe throughout the eighteenth and nineteenth centuries fed curiosity about the local landscape and inspired musings about the connection between contemporary Germans and their “ancestors,” antiquarians and archaeologists were quite cautious about using archaeological evidence to make ethnic claims. Even during the period of German unification, many archaeologists emphasized the local and regional character of their finds and treated prehistory as a general science of humankind. As Maner shows, these alternative perspectives endured alongside nationalist and racist abuses of prehistory, surviving to offer positive traditions for the field in the aftermath of World War II. A fascinating investigation of the quest to turn pre- and early history into history, Germany’s Ancient Pasts sheds new light on the joint sway of science and politics over archaeological interpretation.
Germany: Geographies of Complexity (World Regional Geography Book Series)
by Olaf Kühne Florian WeberThis book addresses the highly differentiated spatial, social, cultural and demographic structure(s) of Germany, with a particular focus on the reciprocal relations between different levels of spatial development. The historical development of Germany serves as a background in order to provide context for the development of spatially relevant ideas and ideals (whether in relation to politics, landscape, or culture). In this regard, questions of divergence and convergence become highly salient. The book makes the complexity of spatial and social developments in Germany comprehensible. The neopragmatic approach adopted here allows bringing together different theoretical strands while providing a basis for independent regional geographic research at the same time. Beginning with an overview of the physical structures of Germany which provides the material point of departure for the societal development of Germany, key aspects of the German history are discussed. Particular attention is paid to the reciprocal influence between material substrate and notions of landscape. Here, specific ‘German’ trajectories of aesthetic and normative conceptions of landscape become clear. A common theme throughout the book are questions of divergence and of efforts towards convergence, which become evident when considering past and present economic, political, and demographic developments. Efforts to tackle current challenges, such as adapting to climate change and mitigating it, or securing raw materials, also become apparent. The complexity of spatial processes in Germany is illustrated in case study regions dealing with the challenges of structural change in traditional industrial regions (such as the Ruhr area), or e.g. efforts of Berlin to position and find itself as the capital of a unified Germany. Overall, the book shows how theory-driven regional geographic research can make spatiotemporal complexities tangible and comprehensible.
Germfree and Gnotobiotic Animal Models: Background and Applications
by Bernard S. WostmannThe germfree animal is reared in the laboratory to be bacteria free; its counterpart, the gnotobiotic animal, is exposed to select microorganisms. The need for such an animal model for use in biomedical studies was first expressed by Pasteur in the late 1800s. Subsequent development of germfree and gnotobiotic animals led to an explosion of studies on the effects of microflora and its components on the physiology and metabolism of the host. Germfree and Gnotobiotic Animal Models brings together the most notable points of early and recent studies and gives reference to the most pertinent literature.
Germinal Centers
by Dinis Pedro CaladoThis volume provides key methods and protocols from laboratories engaged in germinal centers (GC) research with the expectation of stimulating further research, and to aid scientists in the study of GC biology and pathology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Germinal Centers: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Germline Development
by Wai-Yee Chan Le Ann BlombergThe germline is unique in mammals as it is the only cell lineage that undergoes mitosis, meiosis, and differentiation, making these cells amenable to in-depth genomic and mechanistic studies. In Germline Development: Methods and Protocols, expert researchers provide a wide-ranging collection of techniques that can be applied to the study of both the male and female germline. As the techniques used for isolating and studying male and female germ cells are slightly dissimilar, the different technologies are grouped under two sections in this volume, namely, male germline and female germline. This collection brings the field of germline developmental studies together such that readers can find, in a single volume, techniques that allow the isolation of gonads with germ cells at different stages of development, the study of the genomics and epigenomics of germ cells, and an examination of the role of single genes in the developmental potential and function of germ cells. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and the avoidance of known pitfalls. Comprehensive and cutting-edge, Germline Development: Methods and Protocols serves as a comprehensive text to individuals, particularly researchers in reproductive and developmental biology, who are interested in studying cells of the germ lineage.
Germline Development in the Zebrafish: Methods and Protocols (Methods in Molecular Biology #2218)
by Roland DoschThis volume details a wide range of methods, ranging from beginner through advanced, used to further study zebrafish and fish germline.. Chapter guide readers through cultivating and manipulating germ cells, imaging of germline processes and the molecular analysis of their, protein, and RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Germline Development in the Zebrafish: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Germline Stem Cells
by Shree Ram Singh Steven X. HouIn this comprehensive and cutting-edge book, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize and maintain them. The volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells.