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Microbial Limit and Bioburden Tests: Validation Approaches and Global Requirements,Second Edition
by Lucia ClontzIn recent years, the field of pharmaceutical microbiology has experienced numerous technological advances, accompanied by the publication of new and harmonized compendial methods. It is therefore imperative for those who are responsible for monitoring the microbial quality of pharmaceutical/biopharmaceutical products to keep abreast of the latest c
Microbial Lipid Production: Methods and Protocols (Methods in Molecular Biology #1995)
by Venkatesh BalanThe volume explores methods for isolation and characterization of lipids, and their production using various oleaginous organisms. The protocols presented in the book consider sugars derived from different substrates including chemically pre-treated agricultural residues, industrial residues containing lignin, food wastes, and industrial waste water in an approachable format. The book also discusses applications that use oleaginous organisms to transform substrates into a variety of products including bio-crude, high value fatty acids, biofuels such as biodiesel, neutral lipids, volatile fatty acids, and surfactants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Microbial Lipid Production: Methods and Protocols is a valuable resource aimed at novice researchers who are working on improving their knowledge and skills in this developing field.
Microbial Lipids and Biodiesel Technologies
by Le Zhang To-Hung Tsui Yen Wah Tong Ronghou LiuThis book, belonging to energy discipline, summarized the latest research progress in the development of microbial lipids and biodiesel technologies. This book introduced the concept and development of microbial lipids and biodiesel technologies, the microbial lipid technology based on oleaginous yeasts, filamentous fungi, microalgae, bacteria, and thraustochytrids. Meanwhile, this book introduced the inhibition and removal of lignocellulosic hydrolysis inhibitors on microbial fermentation for lipid production, the isolation and screening of high-yield strains of oleaginous microorganisms, the use of metabolic engineering to transform oleaginous microorganisms, the process engineering technologies for optimization and process improvement, the harvesting of microbial cells and the extraction of microbial lipids, the production of crude biodiesel by esterification of microbial lipids, the biodiesel purification technology as well as the challenges and prospects of the industrialization of biodiesel technology based on microbial lipids. Reading this book will help readers comprehensively understand the latest developments in the field of "microbial lipids and biodiesel technologies". This book takes into account the relevant practical engineering technologies and the latest basic scientific research, and can be used as a reference for the researchers, engineers, investors, policy-makers, and students engaged in clean energy, microbial lipids and biodiesel industries.
Microbial Machines: Experiments with Decentralized Wastewater Treatment and Reuse in India
by Kelly D. AlleyAround 2004, members of governmental and nongovernmental organizations, science institutes, and private companies throughout India began brainstorming and then experimenting with small-scale treatment systems that could produce usable water from wastewater. Through detailed case studies, Microbial Machines describes how residents, workers, and scientists interact with technology, science, and engineering during the processes of treatment and reuse. Using a human-machine-microbe framework, Kelly Alley explores the ways that people's sensory perceptions of water—including disgust—are dynamic and how people use machines and microbes to digest wastewater. A better understanding of how the human and nonhuman interact in these processes will enable people to generate more effective methods for treating and reusing wastewater. While decentralized wastewater treatment systems may not be a perfect solution, they alleviate resource stress in regions that are particularly hard hit by climate change. These case studies have broad relevance for solving similar problems in many other places around the world.
Microbial Mats
by Joseph Seckbach Aharon OrenThis book provides information about microbial mats, from early fossils to modern mats located in marine and terrestrial environments. Microbial mats - layered biofilms containing different types of cells - are most complex systems in which representatives of various groups of organisms are found together. Among them are cyanobacteria and eukaryotic phototrophs, aerobic heterotrophic and chemoautotrophic bacteria, protozoa, anoxygenic photosynthetic bacteria, and other types of microorganisms. These mats are perfect models for biogeochemical processes, such as the cycles of chemical elements, in which a variety of microorganisms cooperate and interact in complex ways. They are often found under extreme conditions and their study contributes to our understanding of extremophilic life. Moreover, microbial mats are models for Precambrian stromatolites; the study of modern microbial mats may provide information on the processes that may have occurred on Earth when prokaryotic life began to spread.
Microbial Megaplasmids
by Edward SchwartzMegaplasmids are extrachromosomal genetic elements in the size range of 100 kb and larger. They are found in physiologically and phylogenetically diverse groups of bacteria and archaea. By definition, megaplasmids are not essential for the viability of their hosts under all growth conditions, but paradoxically many megaplasmids carry the genetic information for the defining and characteristic traits of the organism in which they reside. Microbial Megaplasmids reviews our knowledge of the extensively studied representatives, such as the catabolic plasmids of the pseudomonads, the rhizobial Sym plasmids, the Ti plasmids of the genus Agrobacterium and the giant enterobacterial virulence plasmids. It also presents snapshots of more recently discovered megaplasmids. The contribution of megaplasmids to the biology of their hosts is described, highlighting the interactions between megaplasmid and chromosomal genes.
Microbial Metabolic Engineering
by Qiong ChengMetabolic engineering is the practice of genetically optimizing metabolic and regulatory networks within cells to increase production and/or recovery of certain substance from cells. In Microbial Metabolic Engineering: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study metabolic engineering. These include methods and techniques to engineer genes and pathways, use of modern biotechnology tools in microbial metabolic engineering, and examples of metabolic engineering for real world applications such as whole cell biosensors and acetate control in large scale fermentation. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Metabolic Engineering: Methods and Protocols seeks to provide researchers with an overview of key topics on microbial metabolic engineering.
Microbial Metabolism In The Digestive Tract
by M. J. HillIn this book an attempt has been made to give an update on the flora of the human digestive tract and its role in disease. This is a subject that has implications in many disciplines and therefore is aimed at not only microbiologists, but also clinicians, dentists, medical researchers, biochemists, and toxicologists who have a background knowledge of bacteriology but are not necessarily directly involved in research into the metabolic actions of gut bacteria.
Microbial Metabolism of Metals and Metalloids (Advances in Environmental Microbiology #10)
by Christon J. HurstThis book explains the metabolic processes by which microbes obtain and control the intracellular availability of their required metal and metalloid ions. The book also describes how intracellular concentrations of unwanted metal and metalloid ions successfully are limited. Its authors additionally provide information about the ways that microbes derive metabolic energy by changing the charge states of metal and metalloid ions.Part one of this book provides an introduction to microbes, metals and metalloids. It also helps our readers to understand the chemical constraints for transition metal cation allocation.Part two explains the basic processes which microbes use for metal transport. That section also explains the uses, as well as the challenges, associated with metal-based antimicrobials.Part three gives our readers an understanding that because of microbial capabilities to process metals and metalloids, the microbes have become our best tools for accomplishing many jobs. Their applications in chemical technology include the design of microbial consortia for use in bioleaching processes that recover metal and metalloid ions from industrial wastes. Many biological engineering tasks, including the synthesis of metal nanoparticles and similar metalloid structures, also are ideally suited for the microbes. Part four describes unique attributes associated with the microbiology of these elements, progressing through the alphabet from antimony and arsenic to zinc.
Microbial Metabolism of Xenobiotic Compounds (Microorganisms for Sustainability #10)
by Pankaj Kumar AroraXenobiotic compounds including pesticides, nitrophenols, pyridine, polycyclic aromatic compounds and polychlorinated biphenyls are widely spread in environment due to anthropogenic activities. Most of them are highly toxic to living beings due to their mutagenic and carcinogenic properties. Therefore, the removal of these compounds from environment is an essential step for environmental sustainability. Microbial remediation has emerged as an effective technology for degradation of these xenobiotic compounds as microorganisms have unique ability to utilize these compounds as their sole source of carbon and energy. The primary goal of this book is to provide detailed information of microbial degradation of many xenobiotic compounds in various microorganisms.
Microbial Metabolomics
by David J. Beale Konstantinos A. Kouremenos Enzo A. PalomboThis book brings together contributions from global experts who have helped to facilitate the exciting and rapid advances that are taking place in microbial metabolomics. The main application of this field is in clinical and veterinary microbiology, but there is a great potential to apply metabolomics to help to better understand complex biological systems that are dominated by multiple-species microbial populations exposed to changing growth and nutritional conditions. In particular, environmental (e. g. , water, soil), food (e. g. , microbial spoilage, food pathogens), and agricultural and industrial applications are seen as developing areas for microbial metabolomics. As such, the book includes contributions with clinical, environmental, and industrial perspectives.
Microbial Metabolomics: Methods and Protocols (Methods in Molecular Biology #1859)
by Edward E.K. BaidooThis detailed volume includes protocols that represent the breadth of microbial metabolomics approaches to both large-scale and small-scale experiments with intention of highlighting techniques that can be used for applications ranging from environmental microbiology to human disease. Utilizing mass spectrometry as their primary measurement tool, the chapters explore microbial metabolomics, metabolism and microbial physiology, metabolite sample preparation, current analytical techniques used to profile primary and secondary metabolites and lipids, as well as establishing data analysis workflows for targeted metabolomics, untargeted metabolomics, analysis of metabolic fluxes, and genome-scale models. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Microbial Metabolomics: Methods and Protocols serves as an ideal reference for both novice and advanced users and can be adapted to similar analytical platforms or customized to suit the needs of the researcher.
Microbial Metabolomics: Recent Developments, Challenges and Future Opportunities
by Sunny Dhiman Sukhminderjit Kaur Manikant TripathiThis book explores the potential of microbial metabolism in health, agriculture, and environmental technologies, serving as a comprehensive guide to microbial metabolomics with practical applications. It uncovers the complex biochemical processes of microbes, from bacteria to fungi, revealing their impact on biotechnology, environmental sciences, healthcare, and agriculture. Microbes, the unseen drivers of biological processes, offer innovative solutions across these fields. The book examines the rapid advancements in microbial metabolomics and addresses challenges like data integration and metabolite identification, providing insights to unlock its full potential. It caters to the growing demand for advanced resources in microbiology and biotechnology, making it valuable for researchers, students, and professionals in microbiology, biotechnology, and biochemistry, as well as innovators in food security and green technology.
Microbial Metal Respiration
by Andreas Kappler Johannes GescherMicrobes can respire on metals. This seemingly simple finding is one of the major discoveries that were made in the field of microbiology in the last few decades. The importance of this observation is evident. Metals are highly abundant on our planet. Iron is even the most abundant element on Earth and the forth most abundant element in the Earth's crust. Hence, in some environments iron, but also other metals or metalloids, are the dominant respiratory electron acceptors. Their reduction massively drives the carbon cycle in these environments and establishes redox cycles of the metallic electron acceptors themselves. These redox cycles are not only a driving force for other biotic reactions but are furthermore necessary for initiating a number of geochemically relevant abiotic redox conversions. Although widespread and ecologically influential, electron transfer onto metals like ferric iron or manganese is biochemically challenging. The challenge is to transfer respiratory electrons onto metals that occur in nature at neutral pH in the form of metal oxides or oxihydroxides that are effectively insoluble. Obviously, it is necessary that the microbes specially adapt in order to catalyze the electron transfer onto insoluble electron acceptors. The elucidation of these adaptations is an exciting ongoing process. To sum it up, dissimilatory metal reduction has wide-spread implications in the field of microbiology, biochemistry and geochemistry and its discovery was one of the major reasons to establish a novel scientific field called geomicrobiology. Recently, the discovery of potential applications of dissimilatory metal reducers in bioremediation or current production in a microbial fuel cell further increased the interest in studying microbial metal reduction
Microbial Metatranscriptomics Belowground
by Prachi Bhargava Manoj Nath Deepesh Bhatt D. K. ChoudharyThe book emphasizes role of functional microbes in soil to improve fertility and plant health in agro-ecosystem. In this compendium main emphasis is on occurrence and distribution of microbial communities, In situ active microbial quorum in rhizosphere, metratranscriptomics for microflora- and fauna, and fnctional diversity in rhizosphere. The book also highlights the importance of PGPRs in rhizosphere, root endotrophic microbes, functional niche under biotic stress, functional niche under abiotic stress, functional root derived signals, as well as functional microbe derived signals. Approaches deployed in metatranscriptomics, and molecular Tools used in rhizosphere are also discussed in detail. The book presents content is useful for students, academicians, researchers working on soil rhizosphere and as a policy document on sustenance of agriculture.
Microbial Mitigation of Stress Response of Food Legumes
by Krishna Kumar A. Sankaranarayanan N. Amaresan Senthilkumar MurugesanMicrobial Mitigation of Stress Responses of Food Legumes provides knowledge on the impact of abiotic and biotic stress on the agriculture of grain legumes especially pulses and it critically reviews the cutting-edge research in exploring plant microbe interactions to mitigate the stress. It helps in understanding the fundamentals of microbial-mediated management of abiotic and biotic stress in grain legumes. Salient features: Describes the usefulness of microbiome of plant/insects for enhancing the production of grain legumes Focuses on recent advances in microbial methods for mitigating the stress and their application in sustainability of legume production Provides a unique collection of microbial data for the improvement of legume productivity Details microbial metabolites at the gene and molecule levels for plant stress management The reader will get all essential and updated information on various stress factors, crop responses, and microbial-mediated stress management for better food legume production.
Microbial Nanobionics: Volume 1, State-of-the-Art (Nanotechnology in the Life Sciences)
by Ram PrasadMicrobial Nanobionics: Volume 1, State of the Art, discusses a wide range of microbial systems and their utilization in biogenic synthesis of metallic nanoparticles. The rich biodiversity of microbes makes them excellent candidates for potential nanoparticle synthesis biofactories. Through a better understanding of the biochemical and molecular mechanisms of the microbial biosynthesis of metal nanoparticles, the rate of synthesis can be better developed and the monodispersity of the product can be enhanced. The characteristics of nanoparticles can be controlled via optimization of important parameters, such as temperature, pH, concentration and pressure, which regulate microbe growth conditions and cellular and enzymatic activities. Large scale microbial synthesis of nanoparticles is a sustainable method due to the non-hazardous, non-toxic and economical nature of these processes.The applications of microbial synthesis of nanoparticles are wide and varied, spanning the industrial, biomedical and environmental fields. Biomedical applications include improved and more targeted antimicrobials, biosensing, imaging and drug delivery. In the environmental fields, nanoparticles are used for bioremediation of diverse contaminants, water treatment, catalysis and production of clean energy. With the expected growth of microbial nanotechnology, this volume will serve as a comprehensive and timely reference.
Microbial Nanobionics: Volume 2, Basic Research and Applications (Nanotechnology in the Life Sciences)
by Ram PrasadMicrobial Nanobionics: Volume 2, Basic Research Applications continues the important discussion of microbial nanoparticle synthesis with a focus on the mechanistic approach of biosynthesis towards nanobionics. This volume also explores the toxicity of nanomaterials in microbes and their effect on human health and the environment. Special Emphasis is given to the use of polymeric nanomaterials in smart packing for the food industry and agricultural sector. The future of nanomaterials for detection of soil microbes and their interactions and tools for environmental remedies is also comprehensively covered.The rich biodiversity of microbes make them excellent candidates for potential nanoparticle synthesis biofactories. Through a better understanding of the biochemical and molecular mechanisms of the microbial biosynthesis of metal nanoparticles, the rate of synthesis can be better developed and the monodispersity of the product can be enhanced. The characteristics of nanoparticles can be controlled via optimization of important parameters, such as temperature, pH, concentration and pressure, which regulate microbe growth conditions and cellular and enzymatic activities. Large scale microbial synthesis of nanoparticles is a sustainable method due to the non-hazardous, non-toxic and economical nature of these processes.The applications of microbial synthesis of nanoparticles are wide and varied, spanning the industrial, biomedical and environmental fields. Biomedical applications include improved and more targeted antimicrobials, biosensing, imaging and drug delivery. In the environmental fields, nanoparticles are used for bioremediation of diverse contaminants, water treatment, catalysis and production of clean energy. With the expected growth of microbial nanotechnology, this volume will serve as a comprehensive and timely reference.
Microbial Nanobiotechnology: Principles and Applications (Materials Horizons: From Nature to Nanomaterials)
by Shivendu Ranjan Nandita Dasgupta Agbaje Lateef Evariste Bosco Gueguim-KanaThis edited book serves as a vital resource on the contributions of microorganisms to advances in nanotechnology, establishing their applications in diverse areas of biomedicine, environment, biocatalysis, food and nutrition, and renewable energy. It documents the impacts of microorganisms in nanotechnology leading to further developments in microbial nanobiotechnology. This book appeals to researchers and scholars of microbiology, biochemistry and nanotechnology.
Microbial Nanotechnology
by Mahendra RaiThis book provides an account of the biogenic synthesis of nanomaterials by using different microorganisms. The chapters are focused on the biosynthesis of various metal and metal oxide nanosized materials by using bacteria, actinomycetes, fungi, and algae, including mechanisms of microbial synthesis. Other chapters summarize recent developments of microbial-based nanostructures for the management of food-borne pathogens, plant pathogenic fungi, as nutrients, and biomedical applications. Microorganisms are discussed not only as biofactories for the synthesis of nanomaterials but also as removal agents of toxic metals from the environment. Exposure sources and ecotoxicity of microbially synthesized nanoparticles are also discussed.
Microbial Nanotechnology: Green Synthesis and Applications
by Mohammad Azam Ansari Suriya RehmanThis book introduces the principles and mechanisms of the biological synthesis of nanoparticles from microorganisms, including bacteria, fungi, viruses, algae, and protozoans. It presents optimization processes for synthesis of microbes-mediated nanoparticles. The book also reviews the industrial and agricultural applications of microbially-synthesized nanoparticles. It also presents the medical applications of green nanoparticles, such as treating multidrug-resistant pathogens and cancer treatment. Further, it examines the advantages and prospects for the synthesis of nanoparticles by microorganisms. Lastly, it also presents the utilization of microbial-synthesized nanoparticles in the bioremediation of heavy metals.
Microbial Natural Products Chemistry: A Metabolomics Approach (Advances in Experimental Medicine and Biology #1439)
by Taícia Pacheco FillThis book focuses on the importance of omics strategies and de-replication analysis to unveil new molecules from microbial sources with diverse chemical structures and biological functions. Chapters address metabolomics strategies, which will lead to a better understanding of the chemical interactions between microorganisms, plant-microorganisms, and virus-microorganisms. Authors also describe analytical tools used in microbial metabolomics and natural products discovery, in addition to describing a step-by-step protocol to identify and annotate metabolites using various databases and online platforms. The book presents the newest research, tools, and protocols for chemists, biochemists, bio-and chemical engineers, and biotechnologists, among others.
Microbial Niche Nexus Sustaining Environmental Biological Wastewater and Water-Energy-Environment Nexus (Environmental Science and Engineering)
by Maulin P. Shah Senthilkumar Kandasamy Kavitha Subbiah Naveenkumar ManickamIn most of the industries, industrial effluent treatment plants are playing vital roles to ensure the efficient management of industrial effluent for supporting sustainable development of our society. Due to the technological development, new concepts about future wastewater management are being incorporated by process industries in the whole world, including recyclable resources and energy/nutrient recovery from industrial effluent, etc. However, conventional treatment methods including biotechnological methods used in treatment plants are facing a lot of difficulties due to the strict discharging norms and coming out of new-fangled pollutants. Recently, a novel concept microbial niche nexus sustaining biological wastewater treatment was introduced, which can accomplish the significant removal of toxic emerging pollutants by different microbial communities, with the concern of other components like integrated and healthy ecosystem. The book focuses on research related to future potential and progress of microbial niche-based environmental biotechnology such as microbial enrichment, microbial function, system design, new technological developments and its applications. Besides, the book reviews important interconnections between water, energy, and the environment as security in water and energy, and the environment is associated with human beings, natural resources, economic, and environmental sustainability. In addition, the book describes innovative green technologies with the aim of enhancing the present state-of-the-art technologies in the various fields like water, energy, the environment, and the related potential fields of industrial wastewater treatment.
Microbial Omics in Environment and Health
by Rajeshwar P. Sinha Minu Kesheri Swarna Kanchan Travis B. SalisburyThis book describes the basic concepts and recent advances in new discoveries and technologies related to microbial omics and their role in environmental research and human health. The term "omics" refers to a blend of high-throughput analysis and traditional methods including genomics, transcriptomics, proteomics, lipidomics and metabolomics for a variety of applications in the field of life sciences, biomedical sciences, environmental sciences, and related industries. The book aims to fill the existing gap in terms of a comprehensive approach that incorporates recent advances in this dynamic field and provides an answer to why this field requires an interdisciplinary research approach. The focus of this book is on the applications of genomics, epigenomics, metagenomics, integrative omics, machine learning and microbiome research in environmental health, disease, wastewater epidemiology, antibiotic resistance, drug discovery, cancer, production of secondary metabolites of microbial origin, oxidative stress alleviating mechanisms, etc. The overall construction of this book emphasizes three major perspectives, namely, elaborate knowledge from fundamentals to recent advances in technologies about omics in general and its application in microbiology, environment, and health in particular; illustrative figures capable of easy visualization of complex pathways; and serving as a platform for highly demanded bioinformatics strategies including relevant codes, programming scripts, machine learning and use of artificial intelligence strategies needed to streamline the research outcomes. With global contributions from authors from internationally renowned organizations who excel in relevant research, this book will benefit bibliophiles and prospective audiences from the research fraternity, academia, professionals, and experts in the field of life sciences, biomedical as well as industries related to the development of drug design and novel advances in biotechnological applications.
Microbial Pest Control
by Sushil KhetanA complete overview of the technologies and products for microbial-based pest control. It documents the use of genetically altered Bt and transgenic crops, microbial formulations, and synergistic interactions of microbials with synthetic chemicals, as well as the management of Bt foliar applications and Bt genes in transgenic crops. The book includ