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Microbial Steroids: Methods and Protocols (Methods in Molecular Biology #2704)

by José-Luis Barredo Carlos Barreiro

This detailed volume explores experimental laboratory procedures for a wide range of steroid bioconversions. After an overview on the current trends and perspectives, the book continues with sections covering microbial screening and synthetic biology applied to microorganisms able to catabolize sterols, methods on strain characterization, including omics and biochemical analyses, methods of fermentation and biocatalysis for steroids production, as well as a chapter on the medical use of glucocorticoids in cancer patients. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Steroids: Methods and Protocols, Second Edition serves as an ideal reference source for laboratory and industrial professionals, as well as for students in a wide array of biological disciplines.

Microbial Strategies for Crop Improvement

by Javed Musarrat Almas Zaidi Mohammad Saghir Khan

This book presents the multidisciplinary nature and the many fascinating aspects of microbiological approaches for crop improvement in both conventional and stressed soils where quality and safety are the key concerns. The major goal is to provide a cross-section of the latest accomplishments and envisaged future directions in these areas. It gives a holistic view of the basic concepts and practical utility of microbes and thus presents an all-inclusive contemporary treatise on strategic aspects of the diverse microbial communities providing solutions to oodles of customary agronomic problems. This book benefits people working in the area of agronomy, biotechnology, environmental biology, microbiology, plant physiology, plant protection and soil science. The contributions by eminent academicians and professionals ensure a good equilibrium between theory and practice without compromising the basic conceptual framework of the concerned subject.

Microbial Strategies for Techno-economic Biofuel Production (Clean Energy Production Technologies)

by P. K. Mishra Vijai Kumar Gupta Neha Srivastava Manish Srivastava

Biofuels are one of the most sustainable options when it comes to renewable energy sources to replace fossil fuels. Biotechnological processes, such as microbial fermentation, are used to produce energy from waste biomass by converting organic substrates into biofuels. This book discusses practices to improve and enrich various microbial communities in order to enhance sustainable and economical biofuel production. It also evaluates various strategies to develop potential microorganisms and microbial consortia to produce highly efficient biofuels at a relatively low cost.

Microbial Strategies for Vegetable Production

by Almas Zaidi Mohammad Saghir Khan

​This book provides a comprehensive information on basic and applied concepts of microbesial strategies adopted for the improvement of vegetables grown in various production systems. The beneficial role of soil microbes including plant growth promoting rhizobacteria (PGPR), nitrogen fixers, and phosphate-solubilizing bacteria in the improvement of vegetables grown both in normal and contaminated soils is discussed. The role of PGPR in tomato production is dealt separately. The impact of heavy metals on different vegetables and abatement of metal toxicity following metal tolerant PGPR and their consequential impact on vegetables grown in metal polluted soil is discussed. Moreover, recent advances in the management of vegetable diseases employing PGPR are addressed. This volume is therefore of special interest to both academics, professionals and practitioners working in the field of vegetable farming/horticulture, microbiology and plant protection sciences.

Microbial Stress Adaptation and Food Safety

by Vijay K. Juneja Ahmed E. Yousef

The first book to address the subject, Microbial Stress Adaptation and Food Safety emphasizes the implications of stress adaptation and its consequences for food safety. It covers the basic science, kinetics, mechanisms, assessment, and control of stress adaptation and its impact on the safety of foods produced by minimal processing or non-thermal technologies. World renowned experts in the field provide detailed accounts of problems associated with stress adaptation and suggest practical solutions for overcoming these problems.

Microbial Stress Tolerance for Biofuels

by Zonglin Lewis Liu

The development of sustainable and renewable biofuels is attracting growing interest. It is vital to develop robust microbial strains for biocatalysts that are able to function under multiple stress conditions. This Microbiology Monograph provides an overview of methods for studying microbial stress tolerance for biofuels applications using a systems biology approach. Topics covered range from mechanisms to methodology for yeast and bacteria, including the genomics of yeast tolerance and detoxification; genetics and regulation of glycogen and trehalose metabolism; programmed cell death; high gravity fermentations; ethanol tolerance; improving biomass sugar utilization by engineered Saccharomyces; the genomics on tolerance of Zymomonas mobilis; microbial solvent tolerance; control of stress tolerance in bacterial host organisms; metabolomics for ethanologenic yeast; automated proteomics work cell systems for strain improvement; and unification of gene expression data for comparable analyses under stress conditions.

Microbial Styrene Degradation

by Dirk Tischler

This book describes the complex processes involved in styrene degradation by microbes, including highly adaptive microorganisms, the various enzymes involved in styrene biodegradation, new styrene-catabolic routes, novel regulatory mechanisms, and the genes coding for styrene metabolizing enzymes. Numerous biotechnological applications are discussed, such as the development of sustainable eco-friendly technologies as well as the use of styrene degrading microorganisms and their enzymes as a rich resource for biotechnology.

Microbial Surfactants in Pharmaceuticals and Cosmetics

by R. Z. Sayyed Shilpa Mujumdar

Biosurfactants and bioemulsifiers are considered green molecules as they are produced from microbes and are easily degradable as compared to surfactants. They are suitable due to properties such as low toxicity, tolerance to a wide-range pH level and temperature, high surface activity, biodegradability, excellent emulsifying and demulsifying ability. While, caution and care should be exercised in its widespread usage, they are likely to replace chemical surfactants.The book focuses on biosurfactant production from various bacteria, diversity of biosurfactant producing bacteria, and the industrial need of biosurfactants. Fields such as pharmacy, medicine, and cosmetics are covered. It is presented in an easy-to-understand manner, and is well illustrated, and comprises protocols and recent data on the production, formulation and commercialization and other aspects of biosurfactants and bioemulsifiers.

Microbial Surfactants: Volume 2: Applications in Food and Agriculture (Industrial Biotechnology)

by R. Z. Sayyed

Biosurfactants are surface-active biomolecules produced by a wide variety of microorganisms. They can be produced from renewable sources, and possess high surface activity, high specificity, low toxicity, tolerance to pH, temperature and ionic strength, biodegradability, excellent emulsifying and demulsifying ability and antimicrobial activity. Biosurfactants have found applications in several industries including organic chemicals, petrochemicals, mining, metallurgy (mainly bioleaching), agrochemicals, fertilizers, foods, beverages, cosmetics, pharmaceuticals and many others. The main aim of this volume is to highlight concepts, classifications, production and applications of microbial surfactants in food and agriculture. The book provides a comprehensive coverage of fermentation, recovery, genomics and metagenomics of biosurfactant production. It is presented in an easy-to-understand manner, and includes protocols, figures, and recent data on the industrial demand market and economics, and the production of biosurfactants from novel substrates are particularly worthwhile additions. The volume will be useful for students, researchers, teachers, and entrepreneurs in the area of microbial biosurfactants and their applications in food and agriculture.

Microbial Surfactants: Volume 3: Applications in Environmental Reclamation and Bioremediation (Industrial Biotechnology)

by R. Z. Sayyed

Biosurfactants are the surface-active biomolecules produced by a wide range of microorganisms. The enormous diversity of biosurfactants make them an interesting group of molecules. Biosurfactants, are less toxic, eco-friendly and possess amphiphatic structure. Due to these and many other useful properties, biosurfactants play a significant role in oil recovery, waste utilization and bioremediation of industrial effluents; hydrocarbons, pesticides and toxic heavy metals and many other hazardous substances. Biosurfactants offer numerous advantages over chemical surfactants. They are environmentally friendly, can be produced from natural sources, less toxic, possess high specificity, have better foaming and emulsifying properties and survive extreme conditions such as high temperatures, extreme pH and salinity. They are cheaper as they can be produced from industrial waste and from by-products as opposed to chemical surfactants. This volume comprises concepts, classification, production and applications of biosurfactants in oil recovery, environment and clean up, etc. It is an excellent literature on fermentation, recovery, genomics and metagenomics of biosurfactant production. It is presented in an easy-to-understand manner, with well-illustrated diagrams, protocols, figures, and recent data on the production, formulation and commercialization and other aspects of biosurfactants. As such, the book will be useful for students, researchers, teachers, and entrepreneurs in the area of PGPR and its allied fields.

Microbial Surfactants: Volume I: Production and Applications (Industrial Biotechnology)

by Hesham All El-Enshasy

Biosurfactants are the surface-active biomolecules produced by microorganisms. Biosurfactants have gained commercial significance due to their unique properties, such as high surface activity, high specificity, low toxicity, tolerance to pH, temperature and ionic strength, biodegradability, excellent emulsifying and demulsifying ability, antimicrobial activity, ability to work under extreme conditions, and relative ease of preparation. Biosurfactants are used in several industries, including organic chemicals, petroleum, petrochemicals, mining, metallurgy (mainly bioleaching), agrochemicals, fertilizers, foods, beverages, cosmetics, pharmaceuticals and many others. The aim of this book is to highlight key aspects from basics to advanced concepts, classifications, production and applications in various fields such as agriculture, health, bioremediation, industries, pharmaceutical, oil recovery, environment, and nanotechnology. It also serves as an excellent and expansive literature on fermentation, recovery, genomics, and metagenomics of biosurfactant production. The book focuses on the biosurfactant production from bacteria, the diversity of biosurfactant producing bacteria, and industrial need of biosurfactant.

Microbial Symbionts and Plant Health: Trends and Applications for Changing Climate (Rhizosphere Biology)

by Swarnendu Roy Piyush Mathur Rupam Kapoor

This book provides a comprehensive understanding of the complex relationship between microbial symbionts and plants in the era of climate change. It focuses on the plant microbiome associated with different plant organs like roots, leaves, stems, fruit, and seeds, and showcases their significant role in the enhancement of crop yield and protection in a sustainable manner. Concomitantly, acumens to the most emerging trends in plant microbial research that includes rhizosphere engineering and metagenomics are also covered in this title. The association of microbial symbionts with the host offers a wide advantage in terms of acclimatization to varied environmental conditions. A large number of microbes such as cyanobacteria, PGPR, endophytes, and AMF have been shown to improve plant growth and production under the effect of various abiotic and biotic stresses. These microbial symbionts secrete several secondary metabolites, signaling molecules, and hydrolytic enzymes that play a multifarious role in improving plant growth and yield. Moreover, the symbionts have been known to regulate the host responses at the molecular level. Bioprospecting these microbial symbionts will provide an alternative to the chemical-based fertilizers and pave the path for the development of biofertilizers. The book is a suitable reading material for undergraduate and postgraduate students, researchers, and scientists working in the field of agricultural biotechnology, microbiology, mycology and plant pathology, and allied fields of plant and microbial sciences. The book in this context attempts to provide an integrative and exhaustive study as well as research material that would help the scientific community in wide respect.

Microbial Synthesis of Chalcogenide Nanoparticles: Combining Bioremediation and Biorecovery of Chalcogen in the Form of Chalcogenide Nanoparticles (IHE Delft PhD Thesis Series)

by Joyabrata Mal

Recent years have seen a growing interest in the application of chalcogenide nanoparticles (NPs), e.g. Se, Te, CdSe and CdTe NPs, in various industrial sectors including energy, petroleum refining and in the field of biology and medicine. Moreover, due to the high toxicity of chalcogen oxyanions, their release into the environment is of great concern. Thus, emphasis was given in this study on the development of a novel microbial synthesis process of chalcogenide NPs by combining biological treatment of Se/Te containing wastewaters with biorecovery in the form of Se NPs, Te NPs and CdSe NPs. Enrichment of Se-oxyanion reducing microorganisms was carried out to simultaneously remove selenite (Se(IV)) and cadmium (Cd(II)) from wastewaters by combining bioremediation of toxic Se-rich wastewater with the biorecovery of Se as CdSe NPs. The results showed compositional changes in the extracellular polymeric substances (EPS) matrix of the anaerobic granular sludge upon exposure to Cd(II) and Se(IV) and identified the roles of EPS fractions in the biogenesis of CdSe NPs. Besides, it was found that the EPS on the surface of the biogenic Se NPs play a major role in lowering the bioavailability and toxicity of biogenic Se(0) compared to chemogenic Se(0) NPs. An upflow anaerobic sludge blanket (UASB) reactor was used for the first time to continuously remove tellurite from wastewater and recover biogenic Te(0).

Microbial Systematics: Taxonomy, Microbial Ecology, Diversity

by Bhagwan Rekadwad

This book presents recent scientific investigations in microbial ecology and systematics. Advanced microbial science investigations employ the latest technologies for research in microbiology and microbial applications. The book has complete information on classical microbiology techniques for assessment of the composition of microbial diversity assessment, advancement in next-generation technology, advantages of microbial products in sustainable developments and their application for societal benefits. Current research on microorganisms is presented as a perfect book for studies on "Microbial Systematics". This book will serve as an important resource for practising research and review for the scientific community.

Microbial Systems Biology

by Ali Navid

Systems biology is the study of interactions between assorted components of biological systems with the aim of acquiring new insights into how organisms function and respond to different stimuli. Although more and more efforts are being directed toward examining systems biology in complex multi-cellular organisms, the bulk of system-level analyses conducted to date have focused on the biology of microbes. In, Microbial Systems Biology: Methods and Protocols expert researchers in the field describe the utility and attributes of different tools (both experimental and computational) that are used for studying microbial systems. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Systems Biology: Methods and Protocols introduces and aids scientists in using the various tools that are currently available for analysis, modification and utilization of microbial organisms.

Microbial Systems Biology: Methods and Protocols (Methods in Molecular Biology #2349)

by Ali Navid

This second edition provides new and updated chapters describing the utility and attributes of different tools that are used for studying microbial systems. Chapters detail a number of methods, including in silico system level analyses by MetaFlux, Kbase genome-scale model builder, COBRA toolbox, NanoSIP, and PAMMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Microbial Systems Biology: Methods and Protocols, Second Edition aims to introduce and aid scientists in using the various tools that are currently available for analysis, modification, and utilization of microbial organisms.

Microbial Technologies for Wastewater Recycling and Management: Recent Trends, Challenges, and Perspectives

by Kumar Vineet

This book introduces the innovative and emerging microbial technologies for the treatment, recycling, and management of industrial, domestic, and municipal water and other wastewater in an environment-friendly and cost-effective manner. It discusses existing methods and technologies, up-gradation of existing technologies, and new technologies. It also highlights opportunities in the existing technologies along with industrial practices and real-life case studies.

Microbial Technologies in Advanced Biofuels Production

by Patrick C. Hallenbeck

Concerns over dwindling fossil fuel reserves and impending climate changes have focused attention worldwide on the need to discover alternative, sustainable energy sources and fuels. Biofuels, already produced on a massive industrial scale, are seen as one answer to these problems. However, very real concerns over the effects of biofuel production on food supplies, with some of ht recent increases in worldwide food costs attributable to biofuel production, have lead to the realization that new, non-food substrates for biofuel production must be bought online. This book is an authoritative, comprehensive, up-to-date review of the various options under development for the production of advanced biofuels as alternative energy sources. A general overview and introductory chapters for each section place the field in the context as well as provide essential basic notions for the more general reader. Accomplished, internationally recognized experts carrying out research on individual focus areas contribute specific technical chapters detailing present progress and future prospects.

Microbial Technologies in Industrial Wastewater Treatment

by Maulin P. Shah

Microbial technology using live, naturally occurring microorganisms in industrial wastewater treatment, is the most effective and innovative method to degrade environmental pollutants such as heavy metals, radionuclides, xenobiotic compounds, organic waste, and pesticides, or to prevent their contamination of the environment. The technology includes several techniques such as biostimulation, biogeneration, bioaccumulation, and biosorption. This book describes the limitations and challenges associated with some generally accepted bioremediation strategies and evaluates the possible applications of these corrective strategies to eliminate toxic pollutants from the environment.The environmental contamination by both natural and anthropogenic sources is, nowadays, an international concern. To decontaminate soils, sediments, and water polluted by anthropogenic activities, scientists and technologists have developed various technologies over the years; however, not only do we have to pay a high cost for physical and chemical environmental technologies but also they sometimes are not ecological or safe. Therefore, we have realized the necessity to develop viable technologies that use microbes and plants to avoid these issues.This book is designed for engineers, scientists, and other professionals who are seeking introductory and advanced knowledge of the principles of nanotechnology, and those who work or are interested in the environmental microbiology or bioremediation field.

Microbial Technology for Health and Environment (Microorganisms for Sustainability #22)

by Pankaj Kumar Arora

Rampant industrialization has caused high levels of contamination by various toxic chemicals in our water bodies, which is a matter of concern in terms of ecosystems, as well as human and animal health. Polluted wastewater can contaminate drinking water and is also is a causal factor for bio-magnification of heavy metals into our food cycle. In the last decade, several methodologies have been adopted to clean the wastewaters, and among these, microbial remediation has emerged as an effective technology. Several variants of microbial technologies have been developed for wastewater treatment and biodegradation specific to the industry, type of waste and toxicity of the chemicals.This book describes the recent advances in microbial degradation and microbial remediation of various xenobiotic compounds in soil and wastewater. It also explains various modern microbial technologies for biodegradation and wastewater treatment. It covers various microbial technologies for wastewater treatment, biodegradation, bioremediation and solid waste management. Gathering contributions from leading international it focuses on the status quo in industrial wastewater treatment and its biodegradation.The book is intended for researchers in the field of industrial wastewater, students of environmental sciences and practitioners in water pollution abatement.

Microbial Technology for Sustainable E-waste Management

by Ravindra Soni Deep Chandra Suyal Saurabh Kumar Prasenjit Debbarma

This book, besides discussing challenges and opportunities, will reveal the microbe-metal interactions and strategies for e-waste remediation in different ecosystems. It will unveil the recent biotechnological advancement and microbiological approach to sustainable biorecycling of e-waste such as bioleaching for heavy metal extraction, valorization of precious metal, biodegradation of e-plastic, the role of the diverse microbial community in e-waste remediation, genetically engineered microbes for e-waste management, the importance of microbial exopolysaccharides in metal biosorption, next-generation technologies, omics-based technologies etc. It also holds the promise to discuss the conservation, utilization and cataloging indigenous microbes in e-waste-polluted niches and promising hybrid technology for sustainable e-waste management.Revolution in the area of information technology and communication is constantly evolving due to scientific research and development. Concurrently, the production of new electrical and electronic equipment also thus uplifting in this era of revolution. These technological advancements certainly have problematic consequences which is the rise of huge amounts of electronic obsoletes or electronic waste (e-waste). Improper management of both hazardous and nonhazardous substances of e-waste led to a major concern in our digital society and environment. Therefore, a sustainable approach including microbial candidates to tackle e-waste is the need of the hour.Nevertheless, the continuous demand for new-generation gadgets and electronics set this high-tech evolution to a new frontier in the last few years. With this continuing trend of technological development, e-waste is expanding exponentially worldwide. In the year of 2019, the worldwide generation of e-waste was approximately 53.6 Mt, of which only about 17.4% of e-waste was collected and recycled, and the other 82.6% was not even documented. E-waste contains various heterogeneous waste complexes such as metals (60%), blends of many polymers (30%) and halogenated compounds, radioactive elements and other pollutants (10%), respectively. The sustainable, efficient, and economic management of e-waste is thus, a challenging task today and in the coming decades. Conventional techniques such as the use of chemicals, incineration and informal ways of e-waste dismantling trigger serious health risks and contamination to the human population and environment, respectively due to the liberation of toxic and hazardous substances from the waste. In this context, bio-candidates especially microorganisms could be sharp-edged biological recycling tools to manage e-waste sustainably. As microbes are omnipresent and diverse in their physiology and functional aspects, they offer a wide range of bioremediation.

Microbial Technology for Sustainable Environment

by Pankaj Bhatt Saurabh Gangola Dhanushka Udayanga Govind Kumar

Microorganisms are ubiquitous on earth. These microorganisms are able to perform various functions in the environment. Microbial applications are used as biofertilizers, bioremediation, biofortification and other sustainable approaches of environmental development. Indigenous microbial cultures have the potential to perform various functions that are beneficial to achieve the sustainable goals. To date, different strains have been commercialized for the industrial and common applications for the sustainable environment. This book will cover different aspects of microbial technology for sustainable development.

Microbial Technology for the Welfare of Society (Microorganisms for Sustainability #17)

by Pankaj Kumar Arora

This book describes various aspects of modern microbiology including microbial enzymes, secondary metabolites, next-generation sequencing, microbial-based biopesticides, microbial-based cancer therapies, biodiesel, and microbial products from fermentation, biodegradation, bioremediation and wastewater treatment. Further, it explains how and why microbes play an important role in preserving the welfare of living beings and the environment. Many bacteria play a significant part in cleaning our environment by detoxifying various xenobiotic compounds, while several microbes produce secondary metabolites that are useful to human beings. The book is divided into 15 chapters that cover various aspects of microorganism-based biotechnology, including recent methodologies such as advanced molecular techniques, as well developments in classical microbiological techniques. The authors also explain how the latest and classical techniques are being used in modern-day microbial biotechnology. All chapters were written by experts from prominent universities, research laboratories, and institutes around the globe. Above all, they focus on recent advances in microbial technology that promote the welfare of living beings and the environment.

Microbial Toxins

by Otto Holst

Interest in the field of microbial toxins is ever growing and spreading across a broad spectrum of scientific disciplines. In an effort to supplement the available reference texts on toxins, Microbial Toxins: Methods and Protocols includes protocols on mold fungus toxins, with some focus on aflatoxins. Intended to support a wide variety of researchers, Microbial Toxins: Methods and Protocols presents the reader with biological, chemical, physical, and medical approaches, as well as state-of-the-art research techniques. Divided into three convenient sections, this detailed volume covers bacterial protein toxins, endotoxins, and mold fungus toxins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Microbial Toxins: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential field.

Microbial Toxins in Food Systems: Causes, Mechanisms, Complications, and Metabolism

by Abdel Moneim Elhadi Sulieman Nawaf Ibrahim Alshammari

When our food items become contaminated with pathogenic microorganisms, these microorganisms secrete microbial toxins which promote infection by attacking the host tissue’s immune system, thereby leading to foodborne intoxication, or poisoning, in consumers. Because these toxic microorganisms are not typically identifiable by taste, smell or sight, it is crucial to the safety of our food systems that they be detected through microbial testing. As the title suggests, Microbial Toxins: Causes, Mechanisms, Complications and Metabolism is a comprehensive overview of the life of these toxins from their pathogenesis through to their implications for human and environmental health. Including examples of salmonella, botulism, listeria and more, as well as various mycotoxins, this text will appeal to both microbiology researchers as well as food industry professionals. Beyond foodborne illness, this text also unpacks environmental toxicology and the role of microbial toxins in the development of novel anti-cancer drugs. Emerging techniques in the detection of microbial toxins will be discussed, setting this text apart from existing books on the subject. The use of proteomics in toxin identification, for example, allows for the determination of metabolic pathways and biomarkers of pathogenicity and resistance of biotoxins. This text furthers the study of foodborne hazards and has important implications for the improvement of safety in the food industry.

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