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The Pauson-Khand Reaction
by Ramon Rios TorresThe Pauson-Khand reaction is an important reaction in the field of organic chemistry. It involves the transition-metal catalysed cycloaddition of an alkyne, an alkene and carbon monoxide, to produce cyclopentenones. The importance of this reaction originates from its high value in transforming simple components into the synthetically useful cyclopentenone unit, in which a high degree of molecular complexity can be achieved in a single step, with impressive stereochemical and regiochemical control.The Pauson-Khand Reaction investigates the nature and many variations of this reaction.Topics covered include: the mechanisms of Pauson-Khand-type reactionsnon chiral intramolecular and intermolecular versions of Pauson-Khand reactionsasymmetric Pauson-Khand reaction using chiral auxiliariesthe enantioselective Pauson-Khand reactionPauson-Khand reactions catalysed by metals other than cobaltunconventional Pauson-Khand reactionsthe Pauson-Khand reaction in total synthesisPresenting a comprehensive overview of this fundamental reaction, The Pauson-Khand Reaction will find a place on the bookshelves of any organic or organometallic chemist.
Pavement Drainage: Theory and Practice
by G L Babu Prithvi S. Kandhal Nivedya Mandankara Kottayi Rajib Basu Mallick Amirthalingam VeeraragavanSUMMARY This book provides complete coverage of surface and subsurface drainage of all types of pavements for highways, urban roads, parking lots, airports, and container terminals. It provides up-to-date information on the principles and technologies for designing and building drainage systems and examines numerous issues, including maintenance and designing for flood events. Practical considerations and sophisticated analysis, such the use of the finite element method and unsaturated soil mechanics, anisotropy and uncertainties, are presented. This book allows civil engineers to make the best use of their resources to provide cost effective and sustainable pavements. Features Presents a holistic consideration of drainage with respect to pavement performance. Includes numerous practical case studies. Examines flooding and the impacts of climate change. Includes PowerPoint slides which include quizzes, schematics, figures, and tables.
Pavement Materials and Associated Geotechnical Aspects of Civil Infrastructures: Proceedings of the 5th GeoChina International Conference 2018 – Civil Infrastructures Confronting Severe Weathers and Climate Changes: From Failure to Sustainability, held on July 23 to 25, 2018 in HangZhou, China (Sustainable Civil Infrastructures)
by WJvdM Steyn Irina Holleran BooHyun NamThis book contains latest research studies regarding issues related to civil infrastructure such as pavement layers and material properties. It contains research data and conclusions that should lead to more resilient infrastructure design, maintenance and management. Civil engineering researchers and practitioners will gain valuable information from this material. Papers were selected from the 5th GeoChina International Conference on Civil Infrastructures Confronting Severe Weathers and Climate Changes: From Failure to Sustainability, held on July 23-25, 2018 in HangZhou, China.
Pavlov's Physiology Factory: Experiment, Interpretation, Laboratory Enterprise
by Daniel P. TodesRussian physiologist and Nobel Prize winner Ivan Pavlov is most famous for his development of the concept of the conditional reflex and the classic experiment in which he trained a dog to salivate at the sound of a bell. In Pavlov's Physiology Factory: Experiment, Interpretation, Laboratory Enterprise, Daniel P. Todes explores Pavlov's early work in digestive physiology through the structures and practices of his landmark laboratory—the physiology department of the Imperial Institute for Experimental Medicine. In Lectures on the Work of the Main Digestive Glands, for which Pavlov won the Nobel Prize in 1904, the scientist frequently referred to the experiments of his coworkers and stated that his conclusions reflected "the deed of the entire laboratory." This novel claim caused the prize committee some consternation. Was he alone deserving of the prize? Examining the fascinating content of Pavlov's scientific notes and correspondence, unpublished memoirs, and laboratory publications, Pavlov's Physiology Factory explores the importance of Pavlov's directorship of what the author calls a "physiology factory" and illuminates its relationship to Pavlov's Nobel Prize-winning work and the research on conditional reflexes that followed it.Todes looks at Pavlov's performance in his various roles as laboratory manager, experimentalist, entrepreneur, and scientific visionary. He discusses changes wrought by government and commercial interests in science and sheds light on the pathways of scientific development in Russia—making clear Pavlov's personal achievements while also examining his style of laboratory management. Pavlov's Physiology Factory thus addresses issues of importance to historians of science and scientists today: "big" versus "small" science, the dynamics of experiment and interpretation, and the development of research cultures.
Payment for Environmental Services in Agricultural Landscapes
by David Zilberman Takumi Sakuyama Leslie Lipper Randy StringerIn recent years, development policy has responded to an increasing concern about natural resource degradation by setting up innovative payment for environmental services (PES) programs in developing countries. PES programs use market and institutional incentives in order to meet both environmental and poverty alleviation objectives. However, their optimal design, implications for the rural poor, and how these initiatives integrate into international treaties on global warming and biodiversity loss are still being discussed. This book addresses these issues by scrutinizing analytical tools, providing policy insights and stimulating debate on linkages between poverty alleviation and environmental protection. In particular, it turns attention towards the role of environmental services in agricultural landscapes as they provide a living for many poor in developing countries. It serves as a valuable reference for academics and students in various disciplines, as well as for policy makers and advisors. This book is a co-publication between Springer and the Food and Agriculture Organization of the United Nations.
PBX Security and Forensics
by I. I. AndroulidakisPBX Security and Forensics presents readers with theoretical and practical background for Private Branch Exchanges (PBXs). PBX is privately owned equipment that serve the communication needs of a private or public entity making connections among internal telephones and linking them to other users in the Public Switched Telephone Network (PSTN). Targeted damages and attacks in PBXs can cause significant instability and problems. The author provides examples of these threats and how to prevent against such attacks in the future. Readers will also be shown where to find forensics data and how to conduct relevant analysis.
PbZn 2020: 9th International Symposium on Lead and Zinc Processing (The Minerals, Metals & Materials Series)
by J. Grogan A. Siegmund S. Alam U. Kerney E. ShibataEstablished in 1970, the PbZn symposium series is considered the leading international technical forum for the lead and zinc processing industries. The PbZn 2020 volume addresses all aspects of current processing technologies for primary and secondary lead and zinc, as well as emerging technologies for both metals.
PCAT Test Prep Flash Cards: Chemistry Essentials (Exambusters PCAT Workbook #4 of 4)
by Ace Inc.<P><P><i>Advisory: Bookshare has learned that this book offers only partial accessibility. We have kept it in the collection because it is useful for some of our members. Benetech is actively working on projects to improve accessibility issues such as these.</i><P><P> 700 questions and answers. Essential definitions, formulas, concepts, and sample problems. <P><P>Topics: Introduction, Matter, Atoms, Formulas, Moles, Reactions, Elements, Periodic Table, Electrons, Chemical Bonds, Heat, Gases, Phase Changes, Solutions, Reaction Rates, Equilibrium, Acids and Bases, Oxidation and Reduction, Introduction to Organic Chemistry, Radioactivity <P>Exambusters PCAT Prep Workbooks provide comprehensive, fundamental PCAT review--one fact at a time--to prepare students to take practice PCAT tests. Each PCAT study guide focuses on one specific subject area covered on the PCAT exams. From 300 to 600 questions and answers, each volume in the PCAT series is a quick and easy, focused read. Reviewing PCAT flash cards is the first step toward more confident PCAT preparation and ultimately, higher PCAT exam scores!
PCAT Test Prep Flash Cards: Biology Essentials (Exambusters PCAT Workbook #3)
by Ace Inc.<P><P><i>Advisory: Bookshare has learned that this book offers only partial accessibility. We have kept it in the collection because it is useful for some of our members. Benetech is actively working on projects to improve accessibility issues such as these.</i><P><P> 450 questions and answers (ILLUSTRATED). Essential definitions and concepts. <P><P>Topics: Cells, Biochemistry and Energy, Evolution and Classification, Kingdoms: Bacteria, Fungi, Protista; Kingdom: Plantae, Kingdom: Animalia, Human Locomotion, Human Circulation and Immunology, Human Respiration and Excretion, Human Digestion, Human Nervous System, Human Endocrinology, Reproduction and Development, Genetics, Ecology <P>Exambusters PCAT Prep Workbooks provide comprehensive, fundamental PCAT review--one fact at a time--to prepare students to take practice PCAT tests. Each PCAT study guide focuses on one specific subject area covered on the PCAT exams. From 300 to 600 questions and answers, each volume in the PCAT series is a quick and easy, focused read. Reviewing PCAT flash cards is the first step toward more confident PCAT preparation and ultimately, higher PCAT exam scores!
PCM-Based Building Envelope Systems: Innovative Energy Solutions for Passive Design (Green Energy and Technology)
by Benjamin DurakovićPCM Enhanced Building Envelopes presents the latest research in the field of thermal energy storage technologies that can be applied to solar heating and cooling with the aim of shifting and reducing building energy demand. It discusses both practical and technical issues, as well as the advantages of using common phase change materials (PCMs) in buildings as a more efficient, novel solution for passive solar heating/cooling strategies. The book includes qualitative and quantitative descriptions of the science, technology and practices of PCM-based building envelopes, and reflects recent trends by placing emphasis on energy storage solutions within building walls, floors, ceilings, façades, windows, and shading devices. With the aim of assessing buildings’ energy performance, the book provides advanced modeling and simulation tools as a theoretical basis for the analysis of PCM-based building envelopes in terms of heat storage and transfer. This book will be of interest to all those dealing with building energy analysis such as researchers, academics, students and professionals in the fields of mechanical and civil engineering and architectural design
Pcr: Methods and Protocols (Methods in Molecular Biology #1620)
by Lucília DominguesThis volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field. Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and synthetic biotechnology, food science and technology, environmental microbiology and molecular ecology, and healthcare. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, PCR: Methods and Protocols hopes to be a valuable and useful resource for wet lab researchers, particularly within the biotechnology and bioengineering field.
PCR: Methods and Protocols (Methods in Molecular Biology #2967)
by Lucília DominguesThis second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
PCR
by Mike McPherson Simon MøllerA thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products a
PCR Cloning Protocols
by Harry W. Janes Bing-Yuan ChenPCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.
PCR Cloning Protocols
by Bruce A. WhiteDistinguished scientists and researchers present a comprehensive collection of current preparative PCR techniques that can be used in cloning and modifying DNA and cDNA. Topics include performing and optimizing PCR (including long PCR), cloning PCR products, cloning unknown neighboring DNA, and library construction and screening. Also covered are mutagenesis, recombination, and in vitro selection, differential and subtractive approaches to cDNA analysis and screening, and cloning members of gene families. The techniques bring to both new and established researchers the power to apply PCR-based methodology to the cloning and modification of DNA, either through innovative protocols or by fostering individual creativity to modify and customize the protocols to best fit their own needs.
PCR Detection of Microbial Pathogens
by Konrad Sachse Joachim FreyHands-on laboratory experts present a set of "classic" PCR-based methods for the identification and detection of important animal and food microbial pathogens, including several zoonotic agents. These proven techniques can be precisely applied to a wide variety of microbes, among them Campylobacter spp., chlamydiae, toxigenic clostridia, Escherichia coli (STEC), Listeria monocytogenes, mycoplasmas, salmonellae, and Yersinia enterocolitica. Additional chapters review the specificity and performance of diagnostic PCR analysis, the pre-PCR processing of samples, the critical aspects of standardizing PCR methods, and the general issues involved in using PCR technology for microbial diagnosis.
PCR Detection of Microbial Pathogens
by Mark WilksPCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one's own tests or introducing tests developed by other workers. PCR Detection of Microbial Pathogens, Second Edition presents alternatives to commercially produced PCR methods to detect microbial pathogens. Although most of the chapters in this book are devoted to the detection of specific pathogens, the first chapters in this book should appeal to anyone working in this field regardless of their particular interests. Although PCR tests can often be made to work with relatively little effort, it is often unclear how efficient the PCR test is, how inhibitory the specimen containing the pathogen of interest is and how the test can be quality controlled. All of which are of great importance in developing tests for diagnostic use. These topics are covered in great depth at the beginning of the book. The main part of the book is devoted to describing methods for the detection of a wide range of pathogens and from widely different specimens and situations. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, PCR Detection of Microbial Pathogens, Second Edition serves microbiologists regardless of their particular interest because, when used together with the general principles, the sheer variety of procedures provided here enables the reader to design and introduce diagnostic tests in the laboratory with confidence.
PCR for Clinical Microbiology
by Catriona L. Halliday Ian W.J. Carter Gregory S. James Margret Schuller Theo P. SlootsNot another textbook, but a valuable tool for doctors and microbiologists wanting to know how to set up a PCR diagnostic microbiology laboratory according to current regulatory standards and perform assays supplied with patient clinical diagnostic criteria and easy to follow protocols. Whether laboratories are using commercial kits or in-house methods developed in their own laboratories or adopted from published methods, all clinical microbiology laboratories need to be able to understand, critically evaluate, perform and interpret these tests according to rigorous and clinically appropriate standards and international guidelines. The cost and effort of development and evaluation of in-house tests is considerable and many laboratories do not have the resources to do so. This compendium is a vehicle to improve and maintain the clinical relevance and high quality of diagnostic PCR. It is a unique collection of; guidelines for PCR laboratory set up and quality control, test selection criteria, methods and detailed step by step protocols for a diagnostic assays in the field of molecular microbiology. The structure of the book provides the PCR fundamentals and describes the clinical aspects and diagnosis of infectious disease. This is followed by protocols divided into; bacteria, virus, fungi and parasites, and susceptibility screens. The inclusion of medical criteria and interpretation adds value to the compendium and benefits clinicians, scientists, researchers and students of clinical diagnostic microbiology
PCR in Bioanalysis
by Stephen J MeltzerPCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.
PCR Primer Design
by Chhandak BasuThis volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.
PCR Primer Design
by Anton YuryevAt the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). This book focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.
PCR Protocols
by John M. Bartlett David StirlingIn this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
PCR Protocols
by Daniel J. ParkKnown for flexibility and robustness, PCR techniques continue to improve through numerous developments, including the identification of thermostable DNA polymerases which exhibit a range of properties to suit given applications. PCR Protocols, Third Edition selects recently developed tools and tricks, contributed by field-leading authors, for the significant value that they add to more generally established methods. Along with the cutting-edge methodologies, this volume describes many core applications, such as PCR cloning and sequencing, expression, copy number or methylation profile analysis, 'DNA fingerprinting', diagnostics, protein engineering, interaction screening as well as a chapter highlighting workflow considerations and contamination control, crucial for all PCR methods. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary reagents and materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, PCR Protocols, Third Edition seeks to further elucidate this essential technique while also providing core principles with broad applications for scientists of all backgrounds.
PCR Protocols
by Bruce A. WhiteThe newest and most up-to-date collection, PCR Protocols offers detailed laboratory procedures for the use of polymerase chain reactions in a wide range of applications. Although specific examples and experimental systems are described, the book's focus is on general applications that can be modified to suit a broad spectrum of research systems, thus making its scope appealing to nearly all researchers. PCR Protocols features clear, easy-to-follow descriptions of procedures and a Notes section in each chapter to provide tips, alternative suggestions, and other enhancements of the protocols. Key Topics and Techniques: basic startup procedures for PCR * PCR in detecting DNA and RNA * PCR in DNA synthesis and mutagenesis * PCR cloning of DNA * selection of primers * radioactive and nonradioactive labeling * quantitation of PCR products by HPLC * chromosome assignment * PCR mapping of HLA genes * sequencing of PCR products * genomic footprinting * quantitation of tumor gene expression * screening of phage libraries * SSP-PCR and genome walking * subcloning of PCR products.
PCR Protocols in Molecular Toxicology (Methods in Life Sciences - Toxicology Section)
by John P. Vanden HeuvelMolecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail: Quantitative reverse transcriptase PCR and methods to examine gene expression Differential display cloning Cloning and library screening by PCR Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes Basic, non-PCR based molecular biology methods PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.