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Plant Toxicology
by Bertold Hock Erich F. ElstnerIn order to keep track of all the compounds and pathogens affecting plant metabolism and development, you would need to spend all your waking hours combing periodicals and the Internet in dozens of languages, as new toxins via pollutants and migratory or mutant pathogens are being discovered every day. Plant Toxicology, Fourth Edition start
Plant Transcription Factors
by Ling Yuan Sharyn E. PerryRecent years have seen significant advancements in the development of enabling technologies that facilitate the study of Transcription Factors (TFs). TFs are pivotal in the regulation of plant development, reproduction, intercellular signaling, response to environment, cell cycle, and metabolism. Plant Transcription Factors: Methods and Protocols offers a comprehensive approach by covering the basic concepts as well as the detailed protocols of a series of commonly used tools for investigating plant TFs. From discussing select TF families in plants to presenting approaches for identifying them, methods are covered to verify the function, to identify protein interactions in which TFs are involved, and how the interactions are mediated. Increasing examples of TFs that function non-cell-autonomously are being discovered and methods to assess intercellular trafficking are also addressed. A section is devoted to examining interaction with DNA, and the volume concludes with a discussion of directed evolution to generate transcription factors that can more efficiently control desired processes. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Transcription Factors: Methods and Protocols serves as an ideal guide to seasoned plant molecular biologists as well as scientists new to the field of TFs and provides many necessary methods to all scientists who are interested in exploring the functions of transcription factors.
Plant Transcription Factors: Methods and Protocols (Methods in Molecular Biology #1830)
by Nobutoshi YamaguchiThis detailed book provides general protocols and technologies that plant biologists worldwide often utilize for the purpose of accelerating research progress in the field of plant transcription factors. Beginning with a brief introduction, the volume continues by exploring methods in the preparation of plant materials, detection of expression levels, interaction tests, and chromatin analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Transcription Factors: Methods and Protocols aims to answer a wide range of questions related to transcription factors commonly raised by plant biologists.
Plant Transformation Technologies
by Jr. Tzvi Tzfira Alisher Touraev Vitaly Citovsky C. Neal StewartPlant Transformation Technologies is a comprehensive, authoritative book focusing on cutting-edge plant biotechnologies, offering in-depth, forward-looking information on methods for controlled and accurate genetic engineering. In response to ever-increasing pressure for precise and efficient integration of transgenes in plants, many new technologies have been developed. With complete coverage of these technologies, Plant Transformation Technologies provides valuable insight on current and future plant transformation technologies. With twenty-five chapters written by international experts on transformation technologies, the book includes new information on Agrobacterium, targeting transgenes into plant genomes, and new vectors and market systems. Including both review chapters and protocols for transformation, Plant Transformation Technologies is vitally important to graduate students, postdoctoral students, and university and industry researchers.
Plant Transformation via Agrobacterium Tumefaciens: Culture Conditions, Recalcitrance and Advances in Soybean
by Phetole MangenaPlant Transformation via Agrobacterium Tumefaciens compiles fundamental and specific information and procedures involving in vitro soybean transformation, which forms the basis for the Agrobacterium-mediated genetic manipulation of soybean using plant tissue culture. This method serves as one of the most preferred, reliable and cost-effective mechanism of transgene expression in both leguminous recalcitrant species and non-legume crops. The technology is favoured due to its simplicity, feasibility and high transformation rates that are so far achieved mostly in monocot plants and a few dicot genotypes. This book provides a comprehensive review of plant transformation which remains necessary for many researchers who are still facing protocol-related hurdles. Among some of the major topics covered in Plant Transformation via Agrobacterium Tumefaciens are the history and discovery of Agrobacterium bacterium, longstanding challenges causing transformation inefficiencies, types and conditions of explants, development of transgenic plants for stress resistance, and the role of transgenic plants on animal/human health, including the environment. Plant Transformation via Agrobacterium Tumefaciens helps the reader to understand how soybean, like many other orphan legume crops, faces the risk of overexploitation which may render the currently available varieties redundant and extinct should its narrow gene pool not improve. Plant transformation serves as a key technique in improving the gene pool, while developing varieties that are drought tolerant, have enhanced nutritional value, pest resistant and reduce the destruction by disease causing microorganims. This book is an essential foundation tool that is available for researchers and students to reinforce the application of Agrobacterium-mediated genetic transformation in soybean.
Plant Transposable Elements
by Josep Casacuberta Marie-Angèle GrandbastienTransposable elements are short lengths of DNA with the capacity to move between different points within a genome. This process can affect the function of genes at or near the insertion site. The present book gives an overview of the impact of transposable elements on plant genomes and explains how to recognize and study transposable elements, e.g. by using state-of-the-art strategies like "new generation sequencing." Moreover, the impact of transposable elements on plant genome structure and function is reviewed in detail, and also illustrated in examples and case studies. The book is intended both for readers familiar with the field and for newcomers. With large-scale sequencing becoming increasingly available, more and more people will come across transposable element sequences in their data, and this volume will hopefully help to convince them that they are not just "junk DNA."
Plant Transposable Elements: Methods and Protocols
by Thomas A. PetersonTransposable elements have played a major role in shaping plant genome structure and gene expression. Transposons not only drive sequence expansion, induce mutations and generate chromosome rearrangements, they also help to shape the epigenetic topology of the eukaryotic genome. In Plant Transposable Elements: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study transposons. These methods include computational approaches to study the ancient transposon remnants that comprise the bulk of plant genomes, as well as laboratory techniques to identify recent and ongoing transposition events. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Transposable Elements: Methods and Protocols seeks to aid scientists in the further study of transposons by providing essential background information and specific experimental protocols.
Plant Transposable Elements: Methods and Protocols (Methods in Molecular Biology #2250)
by Jungnam ChoThis volume details the most up-to-date technologies used in plant transposable element studies and provides easy-to-follow protocols. Chapters guide readers on available database resources, annotation of different families of transposon, and experimental methods to detect their transposition intermediates, neo-transposed DNAs, and transposition events. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Transposable Elements: Methods and Protocols aims to provide web-lab and dry-lab methodologies targeted at various levels from beginner to experienced.
Plant Transposons and Genome Dynamics in Evolution
by Nina V. FedoroffThe transposable genetic elements, or transposons, as they are now known, have had a tumultuous history. Discovered in the mid-20th century by Barbara McClintock, they were initially received with puzzlement. When their genomic abundance began to be apparent, they were categorized as "junk DNA" and acquired the label of parasites. Expanding understanding of gene and genome organization has revealed the profound extent of their impact on both.Plant Transposons and Genome Dynamics in Evolution captures and distills the voluminous research literature on plant transposable elements and seeks to assemble the big picture of how transposons shape gene structure and regulation, as well as how they sculpt genomes in evolution. Individual chapters provide concise overviews of the many flavors of plant transposons and of their roles in gene creation, gene regulation, development, genome evolution, and organismal speciation, as well as of their epigenetic regulation.This volume is essential reading for anyone working in plant genetics, epigenetics, or evolutionary biology.
Plant Vacuolar Trafficking: Methods and Protocols (Methods in Molecular Biology #1789)
by Cláudia PereiraThis volume provides a collection of state-of-the-art protocols on plant vacuolar trafficking. Chapters guide the reader through design and preparation of fusion proteins, expression vectors, and detailed analysis of different microscopy techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Plant Vacuolar Trafficking: Methods and Protocols aims to be a highly valuable resource for researchers interested in learning more about this field
Plant Variation and Evolution
by David Briggs Briggs S. Max Walters David And Walters S. MaxNatural populations of plants show intricate patterns of variation. European botanists of the eighteenth and nineteenth centuries used this variation to classify different 'kinds' into a hierarchy of family, genus, and species. Although useful, these classifications were based on a belief in the fixity of species and the static patterns of variation. Darwin's theory of evolution changed this view; populations and species varied in time and space and were part of a continuing process of evolution. The development of molecular techniques has transformed our understanding of microevolution and the evolutionary history of the flowering plants. This new edition reviews recent progress in its historical context, showing how hypotheses and models developed in the past have been critically tested. The authors consider the remarkable insights that molecular biology has given us into the processes of evolution in populations of cultivated, wild and weedy species, the threats of extinction faced by many endangered species and the wider evolutionary history of the flowering plants as revealed by cladistic methods.
Plant Viral Vectors
by Kenneth Palmer Yuri GlebaIn this volume, the authors provide an excellent overview of how far the plant viral vector field has come. The discipline is no longer exclusively in the domain of academics--there is a small, but growing number of small biotechnology companies that exploit plant viruses as the platform for commercial innovation in crop improvement, industrial product manufacturing, and human and veterinary health care.
Plant Virology Protocols
by Ichiro Uyeda Chikara MasutaPlant Virology Protocols: New Approaches to Detect Viruses and Host Responses addresses recent developments in genome analyses and cytological technologies being used today to learn more about plant virology. Opening with chapters covering techniques relevant to the detection of unknown viruses and disease diagnosis, this detailed volume continues with chapters on the utilization of meta-genome sequencing and global gene expression analyses for the search and identification of viruses, as well as the elucidation of host responses to viral infection, construction methods of infectious cDNAs, and methods relevant to plant virus control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses will be an invaluable guide to researchers working in the field of plant sciences.
Plant Virology Protocols
by Yiguo Hong Peter Nagy Elisabeth Johansen Gary FosterFollowing the considerable success of the first edition of Plant Virology Protocols, this exciting new edition covers the many new techniques that are now applied to the examination and understanding of plant viruses. Each section presents the most novel methods and step-by-step reproducible laboratory protocols to allow researchers more effective approaches to study plant viruses. This updated book will prove indispensable to laboratory investigators studying plant viruses.
Plant Virology Protocols
by Sally Taylor Gary D. FosterA comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus.
Plant Virology: Methods and Protocols (Methods in Molecular Biology #2400)
by Yi Li Aiming WangThis volume discusses traditional and current techniques that are successfully used to diagnose plant viruses and study molecular plant-virus interactions. The chapters in this book cover topics such as in vivo detection of double-stranded RNA, developing rice mutant using CRISPR-Cas9-based technology, protein-protein interaction assays, purification and transfection of protoplasts, protocols for gene silencing, and transmission electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Plant Virology: Methods and Protocols is a valuable resource for plant pathologists, microbiologists, virologists, graduate students, and teachers who are interested in learning more about the developments in plant virology research.
Plant Virus and Viroid Diseases in the Tropics
by K. Subramanya Sastry Thomas A. ZitterAround the globe, besides fungal and bacterial diseases, both virus and viroid diseases have acquired greater importance in the realm of plant pathology and call for effective management measures as they are responsible for heavy yield losses and are a matter of vital importance and concern to farmers, horticulturists, gardeners and foresters. Understanding disease epidemiology is of vital importance for formulating viable disease management practices in a given agro-ecosystem. The development and progress of plant disease epidemics are variable from region to region. Epidemiology is not a static process, but rather a dynamic course that varies with a change in the ecology, host, vector and virus systems.
Plant Virus and Viroid Diseases in the Tropics: Introduction of Plant Viruses and Sub-Viral Agents, Classification, Assessment of Loss, Transmission and Diagnosis
by K. Subramanya SastryPlant virus and sub-viral pathogens pose severe constraints to the production of wide range of economically important crops worldwide. The crops raised both through true seed and vegetative propagated materials are affected with number of virus and virus-like diseases. The virus may enter into plants through seed planting materials or by vectors. Once the virus is in the field, it multiplies and spreads following definite patterns depending upon the nature of the vector and agro-meteorological conditions. Disease free crops and plants are great economic and social importance in feeding the world's population. Detection of virus and sub-viral agents at initial stages of infection is critical to reduce economic losses. For nearly two decades, ELISA and its variants played a major role in large scale virus testing and also in the production of virus-free planting materials.
Plant Viruses: Diversity, Interaction and Management
by Rajarshi Kumar Gaur Smp Khurana Yuri DorokhovPlant viruses are significant as they affect our food supply and are capable of rapidly spreading to new plant species, so a comprehensive study of plant viruses is important in understanding their pathogenesis and prevention. This book focuses on the plant virus evolution, their molecular classification, epidemics and management. The key features in the book includes genome organization, translation and replication, virus-coded proteinases, structure of virus particles, cell receptors and host range, the RNA polymerase, quasispecies dynamics and virus evolution, and its natural habitats.
Plant Viruses: Evolution and Management
by Rajarshi Kumar Gaur Nikolay Manchev Petrov Basavaprabhu L. Patil Mariya Ivanova StoyanovaThis book focuses on the evolution of plant viruses, their molecular classification, epidemics and management, covering topics relating to evolutionary mechanisms, viral ecology and emergence, appropriate analysis methods, and the role of evolution in taxonomy. The currently emerging virus species are increasingly becoming a threat to our way of life, both economically and physically. Plant viruses are particularly significant as they affect our food supply and are capable of rapidly spreading to new plant species. In basic research, plant viruses have become useful models to analyze the molecular biology of plant gene regulation and cell-cell communication. The small size of DNA genome of viruses possesses minimal coding capacity and replicates in the host cell nucleus with the help of host plant cellular machinery. Thus, studying virus cellular processes provides a good basis for explaining DNA replication, transcription, mRNA processing, protein expression and gene silencing in plants. A better understanding of these cellular processes will help us design antiviral strategies for plants. The book provides in-depth information on plant virus gene interactions with hosts, localization and expression and the latest advances in our understanding of plant virus evolution, their responses and crop improvement. Combining characterization of plant viruses and disease management and presenting them together makes it easy to compare all aspects of resistance, tolerance and management strategies. As such, it is a useful resource for molecular biologists and plant virologists alike.
Plant Viruses: Volume I: Structure and Replication
by C.L. MandaharThis book deals with the structure and replication of plant viruses, viroids, satellites of plant viruses, and spiroplasmas and neatly sums up the state of our knowledge about these aspects of these pathogens.Published information about single-stranded positive-sense RNA plant viruses is far greater than for any other group of plant pathogens.The book caters to the needs of students as well as researchers and is illustrated with micrographs, figures of postulated models, and genetic maps
Plant Viruses: Volume II: Pathology
by C.L. MandaharPlant viruses are of considerable interest to the science of biology, and their study has contributed significantly to the elucidation of several mysteries of traditional and molecular biology.
Plant Vitamins
by A. MozafarThis unique book provides a comprehensive, up-to-date collection of information on the genetic factors, agronomic production methods, and environmental factors that impact the content of vitamins in plants. The effect of various biotic and abiotic stress factors is discussed, and the possible role of some vitamins in plant tolerance to stress factors is also investigated. The book features eye-opening data on vast vitamin variations among farmer-cultivated plants, as well as an extensive comparison between foods grown organically and those grown by conventional methods.With increasing evidence supporting the role of some vitamins in reducing risks of various forms of human cancer, this book provides timely information for researchers, teachers, and students in agronomy, horticulture, plant physiology, food sciences, and human nutrition.
Plant and Algae Biomass: Feasible Sources for Biofuel Production
by Khalid Rehman Hakeem Rouf Ahmad Bhat Dig Vijay Singh Fernanda Maria TonelliThis volume discusses how plant and algae organisms play a pivotal role in the transformation of solar energy to essential metabolites, and explores the numerous beneficial roles these metabolites have at an industrial level. It presents information on the utilization of plant and algae for biomass production, and shows how this is a practical option for large scale biofuel production. The book examines how these bio-metabolites can then be used to extract biofuel. Biomass produced from plants and algae can act as the source of feedstock for biofuel production and industrially important compounds. This book also explores that by curtailing culturing cost using wastewater, seawater, and industrial water as a nutrient and water source, biomass becomes an economical energy source. The introductory chapters of the book focus on the appreciative values of a pollution-free atmosphere, with special reference to enhanced greenhouse effect, and then are followed by chapters on the potential of plant and algae as a liquid energy resource. This book targets researchers, graduate students, and energy and fuel industry professionals interested in the plant sciences, biotechnology and renewable energy.