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Plant Metabolomics
by Xiaoquan Qi Xiaoya Chen Yulan WangThis book introduces plant metabolomics, an experimental approach that is important in both functional genomics and systems biology. It can be argued that metabolite data is most closely linked to phenotypes and that changes in metabolite content or metabolic networks can therefore indicate gene function more directly than mRNA transcript or protein based-approaches. Additionally, the identification of metabolic markers has important applications in plant breeding. The book, written by researchers who are active in plant metabolomics in China, not only introduces the fundamental concepts and the latest methodological advances in the field of plant metabolomics, but also details new studies from the respective scientific programs of the authors and thus reflects the current state of domestic plant metabolomics research. Professor Xiaoquan Qi is the principal investigator at the Institute of Botany, CAS. Professor Xiaoya Chen is a member of the Chinese Academy of Science and also is the principal investigator at the Shanghai Institutes for Biological Sciences, CAS. Professor Yulan Wang is leading a team in BioSpectroscopy and Metabolomics at the Wuhan Institute of Physics and Mathematics, CAS.
Plant Metabolomics: Methods and Protocols (Methods in Molecular Biology #1778)
by Carla AntónioThis detailed book aims to address the need for standardizing key steps in plant metabolomics research that is still considered a critical issue in the field. The collection unites diverse mass spectrometry (MS)-based protocols, from sample preparation and extraction of plant primary and secondary metabolites (e.g. a range of sugars, lipids, phytohormones and volatile organic compounds) to metabolite analysis, using exclusively highly sensitive MS-based methodologies, followed by frontline bioinformatics and/or mathematical modeling approaches to produce reliable biological data interpretation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Metabolomics: Methods and Protocols serves both new and well-established researchers in the plant metabolomics field in finding helpful guidelines and useful laboratory-based protocols for setting up their routine plant metabolomics experiments.
Plant Metal and Metalloid Transporters
by Kundan Kumar Sudhakar SrivastavaThis edited book stands as a one place knowledge hub for plant metal(loid) transporters. The book comprehensively covers holistic aspect of metal(loid) transporters involved in uptake and translocation of essential as well as toxic metal(loid)s. Essential and beneficial metal(loid)s are required in every biological process for normal plant growth and development, however in excess they are toxic. There are toxic metal(loid)s also whose accumulation in plants interferes with normal cellular functioning and hampers growth of plants. Hence, metal(loid) uptake and accumulation in plants is a highly regulated phenomenon involving the role of several transporters, enzymes, metabolites, transcription factors and post translational modifications. The book contains chapters from the experts and the contents of the book are presented in simple language and represented through beautiful and scientifically informative figures and tables. This book is of interest to teachers, researchers, doctoral and graduate students working in the area of plant physiology, environmental biotechnology, plant biotechnology metal(loid) stress, phytoremediation and crop biofortification.
Plant Metallomics and Functional Omics: A System-Wide Perspective
by Gaurav SablokMajor portion of the planet earth is covered by seas and oceans representing 96.5% of the planet’s water, playing a detrimental role in sustaining the plant including crop diversity and productivity for human consumption. Water resources contain both soluble and transition metals, which are easily absorbed by plants through roots as a first point of contact and subsequently play important physiological and biological functions in plants. Transition metals such as copper (Cu), iron (Fe), manganese (Mn) and zinc (Zn) contribute to the plant productivity by playing key functional roles in the photosynthesis. In addition, to their major role in regulating the plant productivity, they also play an important role by acting as homeostatic regulators in uni-parentally inherited chloroplasts and maintains the flow of the electron transfer. It is worthwhile to mention that they play a critical role as transporters, which acts as electron balancing units for managing the electrostatic potential across the membranes. In contrast, some metals such as Cd, As play a significant role in inducing the stress mechanism and influencing either directly or in-directly Haber-Weiss reactions either through the production of the reactive oxygen species (ROS) or through the membrane damage thus leading to leakage of membrane transporters. However, besides playing a detrimental role as transporters in plant system, excessive accumulation of these metals due to the increasing contamination in the marginal soil and water are posing important threats to the plant system. Realizing the toxic effects of the metals, several physiological evidences have been laid for the credence of the metal toxicity and their concurrent effect on plant productivity. Increasing effects of the metals as toxicants can have three adverse effects on the populations: population can move, persist via local adaptation or phenotypic plasticity, or die. Next generation sequencing studies have revolutionized our abilities to detect the changes in expression profiles across an array of genes, which can in-turn help to develop early markers of metal induced stress. Plant Metallomics and Functional Omics: A System-Wide Perspective focuses on the applications of the system wide understanding of the biological and functional interplay occurring at the juncture of the metalloid induced stress and toxicity. The main goal of this book is to familiarize the readers with the most up-to-date information on metal-induced physiological changes in plant species.
Plant MicroRNAs
by Pamela J. Green Blake C. MeyersMicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects. In Plant MicroRNAs: Methods and Protocols, experts in the field present chapters that focus on the identification, validation, and characterization of the miRNA class of RNAs, and address important aspects about heterochromatic small interfering RNAs. In addition, the methods contained in this volume emphasize miRNA analyses, but also include ways to distinguish one class of small RNAs from another. As a volume in the highly successful Methods in Molecular BiologyTM series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Plant MicroRNAs: Methods and Protocols provides the research community with a set of protocols that will help advance vital miRNA research for all plant species, both in typical model species and non-model species alike.
Plant MicroRNAs and Stress Response
by Deepu Pandita Anu PanditaMicroRNAs (miRNAs) are small (20–24 nt), single stranded, regulatory RNA molecules or gene regulators of critical transcriptional or post-transcriptional gene regulation in plants in sequence-specific order that respond to numerous abiotic stresses and animals, non-coding, highly evolutionarily conserved and widely distributed throughout the plant kingdom. MiRNAs are master regulators of plant growth and development, development attenuation under various environmental stresses by stress-responsive miRNAs and plant stress responses and tolerance. Drought, salinity, heat, cold, UV radiation, heavy metal, pathogens, pests and other microbial infections affect survival, growth, development, quality, yield, and production of plants. Stress induced miRNAs down regulate their target miRNAs. This down regulation leads to the accumulation and function of positive regulators, highlighting their roles in stress responses and tolerance. Plant miRNA mediated modifications include overexpression or repression of stress-responsive miRNAs and/or their target complementary or partially complementary gene products, miRNA-resistant target genes, target-mimics and artificial miRNAs. Thus, miRNAs may serve as "genomic gold mines", novel, potent and potential targets in plant genetic manipulations and miRNA-based biotechnology will aid plant improvement and crop-plant tolerance to different environmental stresses. This book reviews our recent understanding of plant microRNAs, biogenesis and functions, computational tools and bioinformatics, regulation of plant growth and development, expression studies, and the role of plant miRNAs in various biotic and abiotic stress-response regulation in plants.
Plant MicroRNAs: Methods and Protocols (Methods in Molecular Biology #2900)
by Stefan De FolterThis second volume is a collection of new and updated protocols to study miRNA functions in plants. Chapters guide readers from the identification and detection of plant miRNAs, bioinformatic analyses, to strategies for functional analysis of miRNAs and their targets. Furthermore, it contains a few introductory chapters on plant miRNA functioning and, on their conservation, and evolution. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant MicroRNAs: Methods and Protocols, Second Edition aims to provide protocols to help new researchers while also supporting established researchers to broaden the scope of their investigations.
Plant Microbe Interface
by Ajit Varma Ram Prasad Swati TripathiThis book shares the latest insights into the genetic basis of molecular communication between plants and their microbial consortia. Further, the book highlights the capabilities of the rhizosphere and endosphere, which help manage ecosystem responses to climate change, nutrient cycling and sequestration of carbon; and discusses their application to the development and management of renewable energy sources.In their natural environments, plants are surrounded by a tremendous number of microorganisms. Some microbes directly interact with plants in a mutually beneficial fashion, while others colonize plants solely for their own advantage. In addition, microbes can indirectly affect plants by drastically altering their environments. Understanding the complex nature of the plant-microbe interface (PMI) can pave the way for novel strategies to improve plant productivity in an eco-friendly manner.The PMI approach focuses on understanding the physical, molecular, and chemical interactions between organisms in order to determine their functional roles in biological, physical, chemical and environmental systems. Although several metabolites from plants and microbes have now been fully characterized, their roles in chemical interactions between these associates remain poorly understood, and require further investigation.
Plant Microbe Symbiosis
by Ajit Varma Ram Prasad Swati TripathiThis book provides an overview of the latest advances concerning symbiotic relationships between plants and microbes, and their applications in plant productivity and agricultural sustainability. Symbiosis is a living phenomenon including dynamic variations in the genome, metabolism and signaling network, and adopting a multidirectional perspective on their interactions is required when studying symbiotic organisms. Although various plant-microbe symbiotic systems are covered in this book, it especially focuses on arbuscular mycorrhiza (AM) symbiosis and root nodule symbiosis, the two most prevalent systems. AM symbiosis involves the most extensive interaction between plants and microbes, in the context of phylogeny and ecology. As more than 90% of all known species of plants have the potential to form mycorrhizal associations, the productivity and species composition, as well as the diversity of natural ecosystems, are frequently dependent upon the presence and activity of mycorrhizas. In turn, root nodule symbiosis includes morphogenesis and is formed by communication between plants and nitrogen-fixing bacteria. The biotechnological application of plant–microbe symbiosis is expected to foster the production of agricultural and horticultural products while maintaining ecologically and economically sustainable production systems. Designed as a hands-on guide, this book offers an essential resource for researchers and students in the areas of agri-biotechnology, soil biology and fungal biology.
Plant Microbe Symbiosis- Fundamentals and Advances
by Naveen Kumar AroraPlant microbe interaction is a complex relationship that can have various beneficial impacts on both the communities. An urgent need of today's world is to get high crop yields in an ecofriendly manner. Utilization of beneficial and multifaceted plant growth promoting (PGP) microorganisms can solve the problem of getting enhanced yields without disturbing the ecosystem thus leading to sustainability. For this to achieve understanding of the intricate details of how the beneficial microbes form associations with the host plant and sustain that for millions of years must be known. A holistic approach is required wherein the diversity of microbes associated with plant and the network of mechanisms by which they benefit the host must be studied and utilized. 'Plant Microbe Symbiosis - Fundamentals and Advances' provides a comprehensive understanding of positive interactions that occur between plant and microorganisms and their utilization in the fields. The book reviews the enormous diversity of plant associated microbes, the dialog between plant-microbes-microbes and mechanisms of action of PGP microbes. Utilization of PGPRs as nutrient providers, in combating phytopathogens and ameliorating the stressed and polluted soils is also explained. Importantly, the book also throws light on the unanswered questions and future direction of research in the field. It illustrates how the basic knowledge can be amalgamated with advanced technology to design the future bioformulations.
Plant Microbes Symbiosis: Applied Facets
by Naveen Kumar AroraPlants form mutualistic association with various microorganisms, particularly in the rhizosphere region. The association benefits both the partners in a number of ways. A single plant can support the growth of diverse microbes and in reciprocation these microbes help the plant in several ways. A great deal of knowledge is now available on the mechanisms of action of plant growth promoting microbes in forming association with their partner plant and benefitting it. With ever increasing population and to achieve food security it has become utmost necessary to utilize these friendly microbes to enhance the crop yield and quality in an ecofriendly and sustainable manner. We already know about the huge negative impact of chemicals used in agriculture on the humans and the ecosystems as whole. 'Plant Microbes Symbiosis - Applied Facets' provides a comprehensive knowledge on practical, functional and purposeful utility of plant-microbe interactions. The book reviews the utilization of beneficial microbes for crop yield enhancement and protection against diseases caused by phytopathogens and nutrient deficiencies. The tome also reviews the utility of plant growth promoting microbes in helping the plants to deal with abiotic stresses imposed by climate change and anthropogenic activities. The book showcases how plant-microbe interactions are or can be utilized for reclamation of stressed soils and degradation of pollutants in a most effective and environment friendly manner. It also ascertains the reasons for the below par performance of the microbial based inoculants. The utilization of biotechnological tools for development of next generation bioformulations to combat the new challenges and overcome past hurdles has been discussed. This wonderful association between plants and microbes if used properly will not only enhance the crop yields and reclaim barren lands but also make our planet a better place to live on for all of its habitants.
Plant Microbiology
by Andrew Holmes Michael GillingsPlant Microbiology provides a comprehensive source of information on DNA sequencing and mapping, the newest technology and procedures in areas such as radiation hybrid mapping, FISH and specialized sequencing techniques are covered. The book also describes how transgene expression is controlled in plants and how advanced information strategies can be used to manipulate and modify the plant genome. An exciting final chapter provides and overview of all the applications of plant transformation in agriculture, medicine and industry.
Plant Microbiome Engineering (Methods and Protocols in Food Science)
by Dhanasekaran Dharumadurai A. Sankara NarayananThis volume provides lab-oriented protocols to deal the various plant microbiome engineering approaches in a lucid and simple manner. Chapters are divided into four section detailing plant associated microbiomes, single cell genomics, whole community metagenomics, metabolic network monitoring and advanced methods in plant microbiome engineering. Written in the format of the Methods and Protocols in Food Science series, the chapters include an introduction to the respective topic, list necessary materials and reagents, detail well-established and validated methods for readily reproducible laboratory protocols and contain notes on how to avoid or solve typical problems. Authoritative and cutting-edge, Plant Microbiome Engineering aims to provide well-established protocols and procedures largely used by both academics and industrials.
Plant Microbiome Paradigm
by Ajit Varma Ram Prasad Swati TripathiThis book provides a comprehensive overview of the current state of knowledge on plant-microbiome interactions and associations. It covers all major mechanistic approaches used to investigate microbes’ impacts on plant growth promotion, disease control and health.The industrial manufacture of nitrogen currently accounts for roughly 2% of the world’s total energy consumption. Microbial products are expected to reduce the need for costly fertilizers, as well as chemical pesticides and fungicides. While beneficial microorganisms are increasingly being used in agriculture, abiotic and biotic stresses such as heat, drought, cold, and salt can quickly kill or render them useless in the field. However, discovering new and better treatments is a lengthy process due to the considerable microbial diversity found in soils.Researchers have now proposed using biotechnological approaches to accelerate the process of microbial technology development. The fact that plant-associated microbes stimulate plant growth and development is well known, as the examples of rhizobia and mycorrhizal fungi show. The mechanisms by which these microorganisms maintain plant growth include the production of phytohormones, fixation of nitrogen, and the mobilization of phosphorus and minerals. The plant microbiome is also involved in pathogen suppression, and especially the root microbiome acts as a protective shield against soil-borne pathogens.A special feature of this book is its multidisciplinary approach, spanning from plant microbiology/biocontrol, fungal and bacterial endophytes, plant physiology, to biochemistry, proteomics and genomics. It is ideally suited for researchers and student of agri-biotechnology, soil biology and fungal biology.
Plant Microbiome and Biological Control: Emerging trends and applications (Sustainability in Plant and Crop Protection #20)
by Swarnendu Roy Piyush MathurThis book offers a comprehensive guide to discovering, assessing, and utilizing consortia of beneficial microbes for crop protection and enhanced crop production in the context of climate change. It provides deep insights into the functional roles of the rhizomicrobiome, including AMF, endophytes, PGPRs, and the phyllomicrobiome, as well as the microbiomes of different plant parts such as seeds, fruits, and stems, in promoting plant growth, development, and the biocontrol of pests and pathogens in a sustainable manner. The book also presents the latest updates on molecular biology techniques, genetic engineering, biotechnological tools, and metagenomics, which are widely used for analyzing plant-pathogen interactions and microbial identification. It will be especially valuable for students and faculty involved in the study and teaching of plant–microbe interactions, as well as researchers working on sustainable methods for plant disease management. With cutting-edge research from leading experts, this book aims to contribute to the development of an eco-friendly, sustainable agricultural system.
Plant Microbiome for Plant Productivity and Sustainable Agriculture (Microorganisms for Sustainability #37)
by Narendra Tuteja Naga Raju Maddela Ram Prasad Sagar ChhabraThis edited book deals with latest comprehensive information on conventional and high throughput techniques and technologies that are recently used to study plant microbial interface for agricultural research and enhancing plant productivity. Plant microbiota are important for many plant growth promotion activity and agricultural productivity and are sustainable green technology for enhancing agricultural productivity under changing environment. The book covers recent information about the plant associated microbiota and their ecology. It discusses technologies to isolate and test microbiota inhabiting in different portion of plants. The book explores the conventional methods as well as the most recently recognized high throughput technologies which are important for productive agroecosystems to feed the growing global population.This book is of interest to teachers, researchers, microbiologist, plant and environmental scientist and those interested in environment stewardship around the world. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, soil science, and environmental sciences and policy makers to be a useful to read.
Plant Microbiome: Stress Response
by Parvaiz Ahmad Dilfuza EgamberdievaThis book presents state-of-the-art research on the many facets of the plant microbiome, including diversity, ecology, physiology and genomics, as well as molecular mechanisms of plant-microbe interactions. Topics considered include the importance of microbial secondary metabolites in stimulating plant growth, induced systemic resistance, tolerance to abiotic stress, and biological control of plant pathogens. The respective contributions show how microbes help plants to cope with abiotic stresses, and represent significant progress toward understanding the complex regulatory networks critical to host-microbe interaction and plant adaptation in extreme environments. New insights into the mechanisms of microbial actions in inducing plant stress tolerance open new doors for improving the efficacy of microbial strategies, and could produce new ways of economically increasing crop yields without harming the environment. As such, this book offers an essential resource for students and researchers with an interest in plant-microbe interaction, as well as several possibilities for employing the plant microbiome in the enhancement of crop productivity under future climate change scenarios.
Plant Microbiomes for Sustainable Agriculture (Sustainable Development and Biodiversity #25)
by Joginder Singh Ajar Nath Yadav Ali Asghar Rastegari Neelam YadavThis book encompasses the current knowledge of plant microbiomes and their potential biotechnological application for plant growth, crop yield and soil health for sustainable agriculture. The plant microbiomes (rhizospheric, endophytic and epiphytic) play an important role in plant growth, development, and soil health. Plant and rhizospheric soil are a valuable natural resource harbouring hotspots of microbes, and it plays critical roles in the maintenance of global nutrient balance and ecosystem function. The diverse group of microbes is key components of soil–plant systems, where they are engaged in an intense network of interactions in the rhizosphere/endophytic/phyllospheric. The rhizospheric microbial diversity present in rhizospheric zones has a sufficient amount of nutrients release by plant root systems in form of root exudates for growth, development and activities of microbes. The endophytic microbes are referred to those microorganisms, which colonize in the interior of the plant parts, viz root, stem or seeds without causing any harmful effect on host plant. Endophytic microbes enter in host plants mainly through wounds, naturally occurring as a result of plant growth, or through root hairs and at epidermal conjunctions. Endophytes may be transmitted either vertically (directly from parent to offspring) or horizontally (among individuals). The phyllosphere is a common niche for synergism between microbes and plant. The leaf surface has been termed as phyllosphere and zone of leaves inhabited by microorganisms as phyllosphere. The plant part, especially leaves, is exposed to dust and air currents resulting in the establishments of typical flora on their surface aided by the cuticles, waxes and appendages, which help in the anchorage of microorganisms. The phyllospheric microbes may survive or proliferate on leaves depending on extent of influences of material in leaf diffuseness or exudates. The leaf diffuseness contains the principal nutrients factors (amino acids, glucose, fructose and sucrose), and such specialized habitats may provide niche for nitrogen fixation and secretions of substances capable of promoting the growth of plants. The microbes associated with plant as rhizospheric, endophytic and epiphytic with plant growth promoting (PGP) attributes have emerged as an important and promising tool for sustainable agriculture. PGP microbes promote plant growth directly or indirectly, either by releasing plant growth regulators; solubilization of phosphorus, potassium and zinc; biological nitrogen fixation or by producing siderophore, ammonia, HCN and other secondary metabolites which are antagonistic against pathogenic microbes. The PGP microbes belong to different phylum of archaea (Euryarchaeota); bacteria (Acidobacteria, Actinobacteria, Bacteroidetes, Deinococcus-Thermus, Firmicutes and Proteobacteria) and fungi (Ascomycota and Basidiomycota), which include different genera namely Achromobacter, Arthrobacter, Aspergillus, Azospirillum, Azotobacter, Bacillus, Beijerinckia, Burkholderia, Enterobacter, Erwinia, Flavobacterium, Gluconoacetobacter, Haloarcula, Herbaspirillum, Methylobacterium, Paenibacillus, Pantoea, Penicillium, Piriformospora, Planomonospora, Pseudomonas, Rhizobium, Serratia and Streptomyces. These PGP microbes could be used as biofertilizers/bioinoculants at place of chemical fertilizers for sustainable agriculture. The aim of “Plant Microbiomes for Sustainable Agriculture” is to provide the current developments in the understanding of microbial diversity associated with plant systems in the form of rhizospheric, endophytic and epiphytic. The book is useful to scientist, research and students related to microbiology, biotechnology, agriculture, molecular biology, environmental biology and related subjects.
Plant Microevolution and Conservation in Human-Influenced Ecosystems
by David BriggsAs human activities are increasingly domesticating the Earth's ecosystems, new selection pressures are acting to produce winners and losers amongst our wildlife. With particular emphasis on plants, Briggs examines the implications of human influences on micro-evolutionary processes in different groups of organisms, including wild, weedy, invasive, feral, and endangered species. Using case studies from around the world, he argues that Darwinian evolution is ongoing. He considers how far it is possible to conserve endangered species and threatened ecosystems through management, and questions the extent to which damaged landscapes and their plant and animal communities can be precisely recreated or restored. Many of Darwin's ideas are highlighted, including his insights into natural selection, speciation, the vulnerability of rare organisms, the impact of invasive species, and the effects of climate change on organisms. An important text for students and researchers of evolution, conservation, climate change and sustainable use of resources.
Plant Micronutrients: Deficiency and Toxicity Management
by Khalid Rehman Hakeem Tariq AftabPlants require essential nutrients (macronutrients and micronutrients) for normal functioning. Sufficiency range is the levels of nutrients necessary to meet the plant’s needs for optimal growth. This range depends on individual plant species and the particular nutrient. Nutrient levels outside of a plant’s sufficiency range cause overall crop growth and health to decline, due either to deficiency or toxicity from over-accumulation. Apart from micronutrients (B, Cl, Mn, Fe, Zn, Cu and Mo), Aluminum (Al), cerium (Ce), cobalt (Co), iodine (I), lanthanum (La), sodium (Na), selenium (Se), silicon (Si), titanium (Ti), and vanadium (V) are emerging as novel biostimulants that may enhance crop productivity and nutritional quality. These beneficial elements are not "essential" but when supplied at low dosages, they augment plant growth, development, and yield by stimulating specific molecular, biochemical, and physiological pathways in responses to challenging environments.The book is the first reference volume that approaches plant micronutrient management with the latest biotechnological and omics tools. Expertly curated chapters highlight working solutions as well as open problems and future challenges in plant micronutrient deficiency or toxicity. We believe this book will introduce readers to state-of-the-art developments and research trends in this field.
Plant Microtechniques and Protocols
by Edward Chee Tak Yeung Claudio Stasolla Michael John Sumner Bing Quan HuangA proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one's own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e. g. , Jensen (1964); O'Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e. g. , archeology, wood science, etc. ]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.
Plant Migration: The Dynamics of Geographic Patterning in Seed Plant Species
by Jonathan D. SauerUsing cases of plant migration documented by both historical and fossil evidence, Jonathan D. Sauer provides a landmark assessment of what is presently known, and not merely assumed, about the process.
Plant Mineral Nutrients
by Frans J.M. MaathuisThe study of plant mineral nutrition has both academic and applied aspects to it. Today, research into plant mineral nutrition is more pertinent than ever in the face of a growing world population and the increasing need for sustainable agriculture. In Plant Mineral Nutrients: Methods and Protocols, expert researchers in the field detail a comprehensive collection of methodologies that are routinely used in plant mineral nutrition research. These include methods and protocols for plant growth parameters, ion contents and composition, soil analyses, flux measurements and the use of public facilities for high throughput analyses. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Mineral Nutrients: Methods and Protocols seeks to aid scientist in the further study of into plants and their mineral nutrients.
Plant Mitochondria
by Frank KempkenMitochondria are the product of a long evolutionary history. It is now a well established fact that mitochondria did evolve from free living bacteria being the common ancestor of both, eukaryotic mitochondria and α-proteobacteria. Advances in genome sequencing, the establishment of in organello and in vitro assays to name only a few, contributed significantly to advances in plant mitochondrial research. Second generation sequencing and the ability to directly sequence and analyse the whole plant transcriptome certainly will help to develop the research on plant mitochondria to another level in the future. In this book the current knowledge about plant mitochondria is presented in a series of detailed chapters, which have been organized in five main sections: (i) dynamics, genes and genomes; (ii) transcription and RNA processing; (iii) translation and import; (iv) biochemistry, regulation and function; and (v) mitochondrial dysfunction and repair. These sections consist of two to five chapters, each written by well-known specialists in the field. This book thus provides a comprehensive inside in the field of plant mitochondria for the specialist. The addition of a glossary and text boxes to each chapter provides easy access for readers from other subjects and hopefully will attract young scientist to the fascinating and exiting field of plant mitochondria.
Plant Mitochondria
by James Whelan Monika W. MurchaThe chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today, starting from the isolation of mitochondria to detailed analyses of RNA, protein and enzymatic activities. Given that the ability to uncover mitochondria's unique features is underpinned by current methodology, this book explores the subject from morphology to detailed molecular mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Mitochondria: Methods and Protocols serves as a vital resource to beginners in the field as well as to expert researchers who find themselves being pulled into the field of mitochondrial research as it links to so many important aspects of plant cell biology.