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Recombinant Protease Inhibitors in Plants
by Dominique MichaudPocket-sized text provides the procedures for taking accurate vital signs. Provides an historical overview and covers such vital signs as temperature, heart rate, respiration, blood pressure, and level of consciousness. For nurses, residents and physicians. Wire-spiral binding.
Recombinant Protein Expression in Mammalian Cells: Methods and Protocols (Methods in Molecular Biology #1850)
by David L. HackerThis detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host; however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.
Recombinant Protein Expression in Mammalian Cells: Methods and Protocols (Methods in Molecular Biology #2810)
by David L. HackerThis fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell surface protein ectodomain expression and for identifying host genes involved in protein production. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols, Second Edition serves as an ideal guide for researchers investigatingprotein structure and function and accelerating the discovery of new therapeutic proteins.
Recombinant Protein Production in Yeast (Methods in Molecular Biology #1923)
by Brigitte Gasser Diethard MattanovichThis volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and post-translational modifications of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications.
Recombinant Protein Protocols
by Rocky S. TuanLeading researchers and experts present wide-ranging methods for detecting and isolating expressed gene products-recombinant proteins. These state-of-the-art techniques describe a large number of molecular tags and labels, including enzymes, ligand-binding moieties, and immunodetectable molecules. There are also methods to detect interactive proteins and gene expression-mediated alterations in cellular activity, as well as chapters on in situ detection of gene expression. When combined with a companion volume by the same editor, Recombinant Gene Expression Protocols, both volumes guide the research worker through the entire journey of recombinant gene expression.
Recombinant Proteins from Plants
by Charles Cunningham Andrew J. PorterThis authoritative collection of basic and advanced protocols permits biotechnologists to use plants rather than single cell fermentation systems for expressing recombinant proteins. The protocols provide clear step-by-step instructions for the cloning, expression, and analysis of a wide variety of recombinant proteins in plants, and for the cloning of genes into a number of different plant species. The book also describes promising experimental techniques that will become increasingly important in the future, and includes techniques for the characterization of medically important proteins expressed in plants.
Recombinant Proteins From Plants
by Veronique Gomord Loïc FayeThe past decade has seen the emergence of plants as a viable alternative to the current production systems for therapeutic proteins. In Recombinant Proteins from Plants, expert researchers explore plants and their potential for the production of increasingly safe, high quality and biologically active complex recombinant pharmaceutical proteins. Chapters present several production platforms, focusing on the largest and most important group of biotechnological products in clinical trials: antibodies and their derived fragments with acknowledged potential for immunotherapy in humans. Due to the shortage of step by step protocols that can be used easily by beginners, this volume aims to fill the void with several chapters of detailed instructions for using the main plant expression systems. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Recombinant Proteins from Plants serves as an ideal guide for those new and seasoned plant scientists, pharmaceutical scientists, and molecular and cellular biologists interested in molecular pharming.
Recombinant Proteins from Plants
by Rima Menassa Igor Kolotilin Jacqueline MacdonaldThis volume provides up-to-date scientific achievements from the world's top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Proteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming.
Recombinant Proteins in Plants: Methods and Protocols (Methods in Molecular Biology #2480)
by Stefan Schillberg Holger SpiegelThis volume provided methods and protocols on recombinant protein production in different plant systems, downstream processing, and strategies to optimize protein expression. Chapters guide readers through recombinant protein production in important plant systems, protein recovery and purification, different strategies to optimise productivity, cloning and fusion protein approaches, and the regulation and freedom to operate analysis of plant-produced proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Proteins in Plants: Methods and Protocols aims to be useful to newcomers and experienced researchers interested in expanding their expertise in the field of plant-based protein production.Chapters 6, 8 and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
The Recombinant University: Genetic Engineering and the Emergence of Stanford Biotechnology
by Doogab YiThe advent of recombinant DNA technology in the 1970s was a key moment in the history of both biotechnology and the commercialization of academic research. Doogab Yi’s The Recombinant University draws us deeply into the academic community in the San Francisco Bay Area, where the technology was developed and adopted as the first major commercial technology for genetic engineering. In doing so, it reveals how research patronage, market forces, and legal developments from the late 1960s through the early 1980s influenced the evolution of the technology and reshaped the moral and scientific life of biomedical researchers. Bay Area scientists, university administrators, and government officials were fascinated by and increasingly engaged in the economic and political opportunities associated with the privatization of academic research. Yi uncovers how the attempts made by Stanford scientists and administrators to demonstrate the relevance of academic research were increasingly mediated by capitalistic conceptions of knowledge, medical innovation, and the public interest. Their interventions resulted in legal shifts and moral realignments that encouraged the privatization of academic research for public benefit. The Recombinant University brings to life the hybrid origin story of biotechnology and the ways the academic culture of science has changed in tandem with the early commercialization of recombinant DNA technology.
Recombineering: Methods and Protocols (Methods in Molecular Biology #2479)
by Christopher R. ReischThis volume explores a collection of methods that studies genome editing across a variety of bacteria, phages, and plants. Chapters in this book cover topics such as scarless DNA recombineering of phage in the lysogenic state; HEMSE; Dup-In and DIRex; recombineering in Staphylococcus aureus; and genome editing with Cas9 in lactobacilli. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Recombineering: Methods and Protocols is a valuable resource for any researchers interested in learning more about this developing field.
Recommendations for Fatigue Design of Welded Joints and Components (IIW Collection)
by A. F. HobbacherThis book provides a basis for the design and analysis of welded components that are subjected to fluctuating forces, to avoid failure by fatigue. It is also a valuable resource for those on boards or commissions who are establishing fatigue design codes. For maximum benefit, readers should already have a working knowledge of the basics of fatigue and fracture mechanics. The purpose of designing a structure taking into consideration the limit state for fatigue damage is to ensure that the performance is satisfactory during the design life and that the survival probability is acceptable. The latter is achieved by the use of appropriate partial safety factors. This document has been prepared as the result of an initiative by Commissions XIII and XV of the International Institute of Welding (IIW).
Recommendations for Fatigue Design of Welded Joints and Components (IIW Collection)
by A. F. Hobbacher J. BaumgartnerThis book presents an enriched exploration of structural fatigue assessment. Now in its updated form, this comprehensive edition delves into foundational principles while introducing extensive revisions and fresh content. Notable enhancements include a refined discussion on stress determination, an expanded section on fatigue resistance, also for welded thin sheets, and a thorough update of crucial chapters such as fatigue assessment using S-N curves at constant and variable amplitudes as well as practical application of fracture mechanics on fatigue of welded joints. The addition of new chapters on high-frequency mechanical impact (HFMI) treatment, insightful statistical considerations based on IIW recommendations, and practical application examples further distinguish this edition. With updated references and meticulous attention to detail, this new edition emerges as an indispensable resource, offering professionals and enthusiasts a deeper understanding of fatigue assessment in structural engineering. Prepared as the result of an initiative by Commissions XIII and XV of the International Institute of Welding (IIW), this book represents a significant contribution to the field.
Recommended Practice for Corrosion Management of Pipelines in Oil & Gas Production and Transportation (European Federation Of Corrosion Publications)
by Bijan KermaniFirst Published in 2017. Pipeline integrity is key to maintaining operational success, safety and security and minimising harm to the environment. Corrosion is a dominant contributory factor to failures, leaks and integrity threats in pipelines. Therefore, its optimum control within an integrity management framework is paramount for the cost-effective design of facilities and ensuring continued, uninterrupted and safe operations within the expected design life. This recommended practice (RP) is a compendium of current best practices and state-of-the-art knowledge by major operators, engineering contractors and service companies involved in hydrocarbon pro-duction and transportation. The RP incorporates some minimum operational requirements and practices to ensure that when man-aging corrosion in pipelines, fundamental principles are followed. It covers management of corrosion for pipelines carrying hydrocarbons, injection water and/or produced water from design to decommissioning. It is structured to follow the logical steps of a basic corrosion management process and makes references to relevant and available international standards and/or recommended practices. It is intended for use by personnel from the petroleum industry having knowledge of corrosion and materials. It is hoped that this RP will prove to be a key reference document for engineers, suppliers and con-tractors working in the oil and gas industry, paving the way for corrosion-free operation of pipelines with the ultimate goal of improving safety, security and minimising the impact on the environment
Reconceiving the Gene: Seymour Benzer's Adventures in Phage Genetics
by Frederic Lawrence Holmes William C. SummersThis book relates how, between 1954 and 1961, the biologist Seymour Benzer mapped the fine structure of the region of the genome of the bacterial virus known as phage T4. Benzer's accomplishments are widely recognized as a tipping point in mid-twentieth-century molecular biology when the nature of the gene was recast in molecular terms. More often than any other individual, he is considered to have led geneticists from the classical gene into the molecular age. Drawing on Benzer's remarkably complete record of his experiments, his correspondence, and published sources, this book reconstructs how the former physicist initiated his work in phage biology and achieved his landmark investigation. The account of Benzer's creativity as a researcher is a fascinating story that also reveals intriguing aspects common to the scientific enterprise.
Reconceptualizing Early Mathematics Learning
by Joanne T. Mulligan Lyn D. EnglishThis book emanated primarily from concerns that the mathematical capabilities of young children continue to receive inadequate attention in both the research and instructional arenas. Research over many years has revealed that young children have sophisticated mathematical minds and a natural eagerness to engage in a range of mathematical activities. As the chapters in this book attest, current research is showing that young children are developing complex mathematical knowledge and abstract reasoning a good deal earlier than previously thought. A range of studies in prior to school and early school settings indicate that young learners do possess cognitive capacities which, with appropriately designed and implemented learning experiences, can enable forms of reasoning not typically seen in the early years. Although there is a large and coherent body of research on individual content domains such as counting and arithmetic, there have been remarkably few studies that have attempted to describe characteristics of structural development in young students' mathematics. Collectively, the chapters highlight the importance of providing more exciting, relevant, and challenging 21st century mathematics learning for our young students. The chapters provide a broad scope in their topics and approaches to advancing young children's mathematical learning. They incorporate studies that highlight the importance of pattern and structure across the curriculum, studies that target particular content such as statistics, early algebra, and beginning number, and studies that consider how technology and other tools can facilitate early mathematical development. Reconceptualising the professional learning of teachers in promoting young children's mathematics, including a consideration of the role of play, is also addressed.
Reconceptualizing STEM Education: The Central Role of Practices (Teaching and Learning in Science Series)
by Richard A. Duschl Amber S. BismackReconceptualizing STEM Education explores and maps out research and development ideas and issues around five central practice themes: Systems Thinking; Model-Based Reasoning; Quantitative Reasoning; Equity, Epistemic, and Ethical Outcomes; and STEM Communication and Outreach. These themes are aligned with the comprehensive agenda for the reform of science and engineering education set out by the 2015 PISA Framework, the US Next Generation Science Standards and the US National Research Council’s A Framework for K-12 Science Education. The new practice-focused agenda has implications for the redesign of preK-12 education for alignment of curriculum-instruction-assessment; STEM teacher education and professional development; postsecondary, further, and graduate studies; and out-of-school informal education. In each section, experts set out powerful ideas followed by two eminent discussant responses that both respond to and provoke additional ideas from the lead papers. In the associated website highly distinguished, nationally recognized STEM education scholars and policymakers engage in deep conversations and considerations addressing core practices that guide STEM education.
Reconciling Human Needs and Conserving Biodiversity: The Lake Tumba, Democratic Republic of Congo (Environmental History #12)
by Bila-Isia InogwabiniProtected areas have often been defined as the backbones of biodiversity conservation. Protected areas have often been defined as the backbones of biodiversity conservation. However, legitimate demands formulated by countries for their economic development, growing human populations, forest fragmentations, and needs of local communities for sustainable livelihoods are also pressing demands on protected areas, stringently pressuring conservation community to identify means to reconcile long term biodiversity conservation and communities’ livelihoods. Hence, integrating conservation activities within the global framework of economic development of countries with high biodiversity had become part of conservation paradigms. Integrated development as a route to conservation, strict protected areas, community managed areas, etc. have been tried but resulted in debatable outcomes in many ways. The lukewarm nature of these results brought ‘landscape approach’ at the front of biodiversity conservation in Central Africa. Since the late 1990s the landscape approach uses large areas with different functional attributes and shifts foundational biodiversity conservation paradigms. Changes are brought to the role traditionally attributed to local communities, aligning sustainable development with conservation and stretching conservation beyond the confines of traditional protected areas. These three shifts need a holistic approach to respond to different conservation questions. There are only a few instances where the landscape experience has been scientifically documented and lessons learnt drawn into a corpus of knowledge to guide future conservation initiatives across Central Africa. To subjugate one biodiversity conservation landscape as one case study emerged as a matter of urgency to present the potential knowledge acquired throughout the landscape experiment, including leadership and management, processes tried, results (at least partially) achieved, and why such and such other process or management arrangement were been chosen among many other alternatives, etc. The challenges of the implementation of the conservation landscape approach needed also to be documented. This book responds to the majority of these questions; drawing its content from the firsthand field knowledge, it discusses these shifts and documents what has been tried, how successful (unsuccessful) it was, and what lessons learnt from these trials. Theoretical questions such as threat index, and ecological services, etc. are also discussed and gaps in knowledge are identified.
Reconciling the Bible and Science: A Primer on the Two Books of God
by Kirk Blackard Lynn MitchellReconciling the Bible and Science acknowledges the Bible as the word of God, demonstrates why there is no conflict between the Bible and science, and shows readers how to accept both.
Reconfigurable Active and Passive Planar Antennas for Wireless Communication Systems (Signals and Communication Technology)
by Shiban Kishen Koul Rajesh K. SinghThis book presents state-of-the-art trends in reconfigurable active and passive planar antennas and their applications in wireless communication systems operating in the frequency band 5-6 GHz. Due to various key features such as multifunction antenna design, compactness, planar nature, and low cost, these technologies are becoming popular for current and future wireless applications. This book discusses different novel antenna designs and their working principles in detail. The modern and future wireless systems require wideband antennas to accommodate various channels in a single band or in separate bands. The carrier aggregation (CA) has been introduced in the modern wireless systems such as LTE-advanced systems and 5G./6G. In CA, a device can use several channels for transmission and reception. The used channels can exist in the same frequency band (intra-band CA) or in distinct bands (inter-band CA). To accommodate more channels, more bandwidth is required within the operating band. For portable devices, circularly polarized (CP) antennas are more advantageous over linearly polarized antennas since in CP antennas, there is low risk of misalignment and, hence encountering interference. Circularly polarized antenna also provides higher link reliability for the portable devices. To provide high data rates, more bandwidth is needed to accommodate more channels. Various multifunction, compact, and wideband antennas for plethora of applications are addressed in detail in this book. The scope of developing reconfigurable active antennas for application in beam switching, beam steering, wireless charging, security systems, etc., is described. This book concludes by giving glimpses of antenna requirements for future wireless communication systems.
Reconfiguring the World: Nature, God, and Human Understanding from the Middle Ages to Early Modern Europe (Johns Hopkins Introductory Studies in the History of Science)
by Margaret J. OslerChange in human understanding of the natural world during the early modern period marks one of the most important episodes in intellectual history. This era is often referred to as the scientific revolution, but recent scholarship has challenged traditional accounts. Here, in Reconfiguring the World, Margaret J. Osler treats the development of the sciences in Europe from the early sixteenth to the late seventeenth centuries as a complex and multifaceted process.The worldview embedded in modern science is a relatively recent development. Osler aims to convey a nuanced understanding of how the natural world looked to early modern thinkers such as Galileo, Descartes, Boyle, and Newton. She describes investigation and understanding of the natural world in terms that the thinkers themselves would have used. Tracing the views of the natural world to their biblical, Greek, and Arabic sources, Osler demonstrates the impact of the Renaissance recovery of ancient texts, printing, the Protestant Reformation, and the exploration of the New World. She shows how the traditional disciplinary boundaries established by Aristotle changed dramatically during this period and finds the tensions of science and religion expressed as differences between natural philosophy and theology.Far from a triumphalist account, Osler’s story includes false starts and dead ends. Ultimately, she shows how a few gifted students of nature changed the way we see ourselves and the universe.
Reconnecting to the Source: The New Science of Spiritual Experience, How It Can Change You, and How It Can Transform the World
by Ervin LaszloRenowned authority on science and philosophy Dr. Ervin Laszlo explores the implications of the new quantum sciences to move beyond the of limits of reality as we know it—and find our way again in our “in-formed,” purposively evolving universe.Reconnecting to the Source is a powerful new book on the science of spiritual experience by Dr. Ervin Laszlo. A well-known figure in the fields of new science, consciousness, and spirituality, Dr. Laszlo has inspired some of today’s most important figures in science and philosophy. In Reconnecting to the Source he unpacks the science behind spiritual experience, investigating the ways in which we can access realms of experience beyond the everyday. It is in these moments, when our conscious minds are in contact or perhaps even overridden by our unconscious selves, that we can explore the depths of spiritual meaning. In addition to a foreword by Deepak Chopra, the book includes new, never before published contributions from a long list of well-known writers and public figures—including Jane Goodall, Barbara Marx Hubbard, Zhi-Gang Sha and many more. Each contributor has written about a unique spiritual experience of their own, sharing moments in their lives that are outside of the boundaries of the usual and reflecting on the importance of these moments. This revolutionary and powerful book will challenge you to reconsider the boundaries of our own experience and change how we look at the world around us. It is a unique, never before available resource for people who want to know how they can consciously align with the forces and “attractors” that governs the universe, and brought us, living, conscious people on the scene in the great processes of evolution that unfold here on Earth.
Reconsideration of Science and Technology I: Reflection on Marx’s View (China Perspectives)
by Liu Dachun Wang Bolu Ding Junqiang Liu YongmouThis volume analyzes Karl Marx’s understanding of science and technology and how it is associated with his focus on the perspective of history and human practice, seeking to illuminate a renewed understanding of science and technology from a Marxist angle. As the first volume of a three-volume set that proposes to reconsider science and technology and explores how the philosophy of science and technology responds to an ever-changing world, the book delves into Marx’s analysis of scientific and technological problems and phenomena across five chapters. The authors explain the positioning of science and technology and the Marxist theoretical perspective of history and practice from which Marx’s views on science and technology derive before an examination of three focal dimensions pertaining to science and technology: productivity, technological alienation and liberty. Not always viewed as central to Marx’s works, discussions on science and technology are often underdeveloped – but a reinterpretation of Marx’s thoughts on the issues corroborates the efficacy of Marxism in terms of understanding today’s world and especially the development of science and technology. The volume will appeal to scholars and students interested in Marxist philosophy, the philosophy of science and technology and topics related to scientific culture.
Reconsideration of Science and Technology II: Scientism and Anti-Scientism (China Perspectives)
by Liu Dachun Ai Zhiqiang Yang HuiliIn reviewing and reconsidering the intellectual history of scientism and antiscientism, the authors assess the process of reasoning and prejudices of these contrasting viewpoints, while discussing the repercussions of scientific hegemony and its contemporary criticism. As the second volume of a three-volume set that proposes to reconsider science and technology and explores how the philosophy of science and technology responds to an ever-changing world, this title focuses on ideological trends centering around scientism and anti-scientism since the 19th century. The six chapters look into the emergence of scientism, instrumental reason, scientific optimism, scientific pessimism, scientific crisis and irrationalism and finally the deconstruction of scientism. The authors provide insight into the connections and biases of these disparate views and critiques, explore the influences of the hegemony of science and contemporary critique of science and evaluate the value of postmodernism and deconstructivism. The volume will appeal to scholars and students interested in the philosophy of science and technology, the ideology of scientism and anti-scientism, modernism and postmodernism, Marxist philosophy and topics related to scientific culture.
Reconsideration of Science and Technology III: An Open World (China Perspectives)
by Liu Dachun Yang Huili Fan ShanshanDrawing on debates from traditional and postmodern thoughts on science and technology, the title builds a new theoretical framework to reconsider science and technology, integrating the opposing viewpoints that either justify science or negate it. As the third volume of a three-volume set that proposes to reconsider science and technology and explores how the philosophy of science and technology responds to an ever-changing world, this final volume seeks to restore the cultural implications of science. Across the six chapters, the authors probe the prospect of a pluralistic scientific culture, including discussions of diversified value choices, the tension between reason and unreason, other binary characteristics of scientific knowledge, including objectivity and uniqueness, universality and locality, as well as the loss, awakening and reconstruction of scientific culture. The authors call for a transformation of scientific culture from a dominant culture to an affirmative one and envision a free and open world of science and technology. The volume will appeal to scholars and students interested in the philosophy of science and technology, the ideology of scientism and anti-scientism, modernism and postmodernism, Marxist philosophy and topics related to scientific culture.