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Zygotic Genome Activation: Methods and Protocols (Methods in Molecular Biology #2923)
by Matthew C. GoodThis volume details cutting-edge techniques used to gain new and deeper insights on the fundamental regulation of zygotic genome activation( ZGA). Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Zygotic Genome Activation: Methods and Protocols aims to serve as a practical reference for researchers interested in early development and genome activation.
Zymography: Biological and Clinical Applications, Volume 2 (Methods in Molecular Biology #2918)
by Raouf A. KhalilVolume 2 describes how to determine the activity of different isozymes, allozymes, and families of proteinases to advance the fields of enzymology and molecular evolution, and provides useful biomarkers for various biological processes, pathological conditions, and clinical disorders. The chapters in Volume 2 are organized in three parts. Part I introduces in situ zymography and localization of bright green-fluorescent gelatinase activity in tissue sections, in situ zymography in formalin-fixed paraffin-embedded and mineralized tissues, and in vivo zymography as an essential activity assay for studying the activity of matrix metalloproteinases (MMPs) in a cell-specific manner in the brain. Part II focuses on biological applications of zymography such as fundamentals of zymography and its applications to the study of biological samples, gelatin zymography to quantify MMP-2 and MMP-9 in complex biological specimens, and detection of proteolytic enzymes in polyacrylamide gels supplemented with diverse biological substrates. Part III focuses on potential clinical applications of zymography, with chapters describing assessment of MMP-2 and MMP-9 hydrolytic activity in preclinical and clinical tissue samples, the use of zymography to assess circulating MMP-2 and MMP-9 in plasma and serum and in pathological conditions, and the use of zymography for the detection of bacterial proteases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Zymography: Biological and Clinical Applications, Volume II is a valuable resource for both experts in the field, as well as new scientists aspiring to learn and perform successful zymography techniques.
Zymography
by Jeff Wilkesman Liliana KurzThis detailed volume examines the latest techniques and protocols associated with zymography, the practice of detecting enzyme activity on electrophoretic gels. Sections cover subjects such as endopeptidase zymography, reverse zymography & in situ zymography, 2D zymography, as well as a variety of special cases. Written in the highly respected Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Zymography: Methods and Protocols serves as an ideal aid for a broad array of researchers from the fields of biological and medicinal science, as well as scientists working in clinical and diagnostic medicine, medical genetics, agricultural entomology, genetic monitoring of environmental pollution, and forensic science.