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Animal Cell Biotechnology: Methods and Protocols (Methods in Molecular Biology #1104)
by Ralf PörtnerThe second edition of this book constitutes a comprehensive manual of new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The chapters are written by world-renowned experts and the volume's five parts reflect the processes required for different stages of production. This book is a compendium of techniques for scientists in both industrial and research laboratories that use mammalian cells for biotechnology purposes.
Animal Cell Biotechnology: Methods and Protocols (Methods in Molecular Biology #2095)
by Ralf PörtnerAnimal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. Special emphasis was put on model-assisted concepts. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line and media development, optimizing process techniques and process strategies, use of model-assisted tools for process design and optimization, and in analysis. Topics include cell line and media development, techniques for process development, model-based techniques for process development, process analysis, and downstream techniques. The volume is divided into five parts that reflect the processes required for different stages of production. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Animal Cell Biotechnology: Methods and Protocols, Fourth Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
Animal Cell Culture
by John M. DavisThis is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible.This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.
Animal Cell Culture (Cell Engineering #9)
by Mohamed Al-RubeaiAnimal cells are the preferred "cell factories" for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libraries.
Animal Cell Culture (Methods in Molecular Biology #5)
by John M. Walker Jeffrey W. PollardAnimal Cell Culture, the latest volume in Humana's highly successful Methods in Molecular Biology series, provides detailed practical techniques for the culture of a broad spectrum of basic cell cell types. Chapters offer hands-on methods for creating mammalian fibroblastic cell cultures and maintaining culture conditions for epithelial, neuronal, and hematopoietic cells among others. Attention is given to the diversity of culture media and extracellular matrices needed to maintain the differentiated functions of the cultured cells. The book's special strength lies in its descriptions of culture techniques for both living and fixed cells. Chapters cover techniques such as: * cinematographic analysis * in situ mRNA hybridization * immunofluorescence * immunoelectron microscopy * somatic cell hybridization * DNA transformation * insect cell culture * creation of hybridoma cell lines * monoclonal antibody techniques * new, specialized methodologies. A useful appendix lists the most commonly used culture media. Comprehensive in scope and coverage, and thoroughly up-to-date, Pollard and Walker's unique handbook on ANIMAL CELL CULTURE is an indispensable resource for both the novice and the seasoned expert.
Animal Cell Culture and Technology (THE BASICS (Garland Science))
by Michael ButlerAnimal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for undergraduates, graduates and experienced researchers. Reflecting the latest developments and trends in the field, the new topics include the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation, and the humanization of antibodies for therapeutic use.
Animal Cell Culture: Principles and Practice (Techniques in Life Science and Biomedicine for the Non-Expert Series)
by Anil Kumar Shalini Mani Manisha SinghThis introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more.
Animal Cell Culture: Principles and Practice (Techniques in Life Science and Biomedicine for the Non-Expert)
by Anil Kumar Shalini Mani Manisha SinghThis introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more.
Animal Cell Electroporation and Electrofusion Protocols (Methods in Molecular Biology #48)
by Jac A. NickoloffThis superb collection of well-tested protocols for the electroporation of proteins and DNA into insect, fish, and mammalian cells also includes proven animal cell electrofusion techniques for studies of somatic cell genetics and development, and for generating monoclonal antibodies. It is distinguished by its coverage of important model cell types from many organisms and tissue types and its detailed instructions for the growth and preparation of specific cells to achieve optimum transfection. Its extensive reference lists, citations of alternative transfer methods, advice on pitfalls to avoid, and descriptions of expected results ensure readily reproducible results even for beginners.
Animal Cell Technology: From Biopharmaceuticals to Gene Therapy
by Michael Butler Leda Castilho Ângela Maria Moraes Elisabeth F. P. AugustoAnimal Cell Technology: from Biopharmaceuticals to Gene Therapy provides a comprehensive insight into biological and engineering concepts related to mammalian and insect cell technology, as well as an overview of the applications of animal cell technology. Part 1 of the book covers the Fundamentals upon which this technology is based and covers the science underpinning the technology. Part 2 covers the Applications from the production of therapeutic proteins to gene therapy. The authors of the chapters are internationally-recognized in the field of animal cell culture research and have extensive experience in the areas covered in their respective chapters.
Animal Cell Technology: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006 (Animal Cell Technology: Basic & Applied Aspects #15)
by Koji Ikura Kiichiro Teruya Sanetaka Shirahata Masaya Nagao Akira IchikawaAnimal cell technology is a growing discipline of cell biology which aims not only to understand structures, functions and behaviors of differentiated animal cells, but also to ascertain their abilities to be used for industrial and medical purposes. The goal of animal cell technology includes the clonal expansion of differentiated cells, the optimization of their culture conditions, modulation of their ability to produce proteins of medical and pharmaceutical importantance, and the application of animal cells to gene therapy, artificial organs and the production of functional foods. This volume gives the readers a complete review of the present state-of-the-art and will be useful for those working in either academic environments or in the biotechnology and pharmaceutical sectors, particularly cell biologists, biochemists, molecular biologists, immunologists, biochemical engineers and all other disciplines related to animal cell culture.
Animal Cells And Life Processes (Investigating Cells)
by Megan Cotugno Barbara SomervillThis book explores the features of the animal cell and includes information about life processes such as respiration.
Animal Characters
by Bruce Thomas BoehrerDuring the Renaissance, horses--long considered the privileged, even sentient companions of knights-errant--gradually lost their special place on the field of battle and, with it, their distinctive status in the world of chivalric heroism. Parrots, once the miraculous, articulate companions of popes and emperors, declined into figures of mindless mimicry. Cats, which were tortured by Catholics in the Middle Ages, were tortured in the Reformation as part of the Protestant attack on Catholicism. And sheep, the model for Agnus Dei imagery, underwent transformations at once legal, material, and spiritual as a result of their changing role in Europe's growing manufacturing and trade economies. While in the Middle Ages these nonhumans were endowed with privileged social associations, personal agency, even the ability to reason and speak, in the early modern period they lost these qualities at the very same time that a new emphasis on, and understanding of, human character was developing in European literature.In Animal Characters Bruce Thomas Boehrer follows five species--the horse, the parrot, the cat, the turkey, and the sheep--through their appearances in an eclectic mix of texts, from romances and poetry to cookbooks and natural histories. He shows how dramatic changes in animal character types between 1400 and 1700 relate to the emerging economy and culture of the European Renaissance. In early modern European culture, animals not only served humans as sources of labor, companionship, clothing, and food; these nonhuman creatures helped to form an understanding of personhood. Incorporating readings of Shakespeare's plays, Milton's Paradise Lost, Margaret Cavendish's Blazing World, and other works, Boehrer's series of animal character studies illuminates a fascinating period of change in interspecies relationships.
Animal Cities: Beastly Urban Histories
by Peter AtkinsAnimal Cities builds upon a recent surge of interest about animals in the urban context. Considering animals in urban settings is now a firmly established area of study and this book presents a number of valuable case studies that illustrate some of the perspectives that may be adopted. Having an ’urban history’ flavour, the book follows a fourfold agenda. First, the opening chapters look at working and productive animals that lived and died in nineteenth-century cities such as London, Edinburgh and Paris. The argument here is that their presence yields insights into evolving understandings of the category ’urban’ and what made a good city. Second, there is a consideration of nineteenth-century animal spectacles, which influenced contemporary interpretations of the urban experience. Third, the theme of contested animal spaces in the city is explored further with regard to backyard chickens in suburban Australia. Finally, there is discussion of the problem of the public companion animal and its role in changing attitudes to public space, illustrated with a chapter on dog-walking in Victorian and Edwardian London. Animal Cities makes a significant contribution to animal studies and is of interest to historical geographers, urban, cultural, social and economic historians and historians of policy and planning.
Animal City
by Joan NegrescolorNina journeys to a secret jungle city populated by animals, plants, and lost objects. The reason for her visit: story hour, where a book's power holds the wild in thrall. The animals are eager for stories about space, the sea, and other worlds. But their favorite story of all is the one told here: a story about a mysterious place, laden with legend and lore, and now overtaken by nature. Five Pantone colors infuse each illustrated spread with a vibrant, electric energy, making this powerful celebration of nature—and stories—as vivid visually as its narrative is engrossing.
Animal City: The Domestication of America
by Andrew A. RobichaudAmerican urbanites once lived alongside livestock and beasts of burden. But as cities grew, human–animal relationships changed. The city became a place for pets, not slaughterhouses or working animals. Andrew Robichaud traces the far-reaching consequences of this shift—for urban landscapes, animal- and child-welfare laws, and environmental justice.
Animal Climate Heroes
by Alison Pearce StevensIn our left corner we have the meanest villain that’s ever existed. Responsible for rising seas and loss of biodiversity, it’s climate change ready to wreak havoc on the Earth. But in our right corner? We have four superheroes ready to save the day!Forest elephants protect our forests by trampling trees.Whales boost ocean health with their massive poo-nados.Sea otters defend kelp forests from purple invaders.And echidnas bury tons of soil to stop climate change.But we can’t leave them in this fight alone. We need to protect our heroes who, in return, defend our planet. Get ready to learn all about these four legged, and two-flippered, creatures and how YOU can be a climate hero too!
Animal Clinical Chemistry: A Practical Handbook for Toxicologists and Biomedical Researchers, Second Edition
by G. O. Evans10+ Years of Updates Since First EditionNewcomers to the animal clinical chemistry and toxicology fields quickly find that the same rules of human medicine do not always apply. Following in the footsteps of its standard-setting first edition, Animal Clinical Chemistry: A Practical Handbook for Toxicologists and Biomedical Researchers, Second Editio
Animal Cognition (Comparative Cognition and Neuroscience Series)
by H. L. Roitblat H. S. Terrace T. G. BeverFirst published in 1984. With this volume we initiate a series of books in comparative cognition and neuroscience. The presentations at the Harry Frank Guggenheim Conference, June 2-4, 1982, out of which the present volume grew, showed that this field of enquiry into cognitive functioning and its neural basis had reached maturity.
Animal Cognition (Routledge Modular Psychology)
by Nick LundAnimal Cognition looks at how non-human animals process information from their environment. Nick Lund has written an accessible and engaging account of this area of comparative psychology. The book contains chapters on animal navigation (including homing behaviour and migration), animal communication methods and research into animal language, and attempts to teach language to non-human animals. A chapter on memory includes models of memory in non-human animals and discusses the importance of memory in navigation and foraging behaviour.Animal Cognition is designed to cover the AQA(A) A2 level specification but will also be of interest to undergraduates new to comparative psychology. It is well illustrated and includes a study aids section with examination questions and answers, and key research summaries.
Animal Cognition: A Tribute To Donald A. Riley (Comparative Cognition and Neuroscience Series)
by Thomas R. ZentallPrepared as a tribute to Donald A. Riley, the essays that appear here are representative of a research area that has loosely been classified as animal cognition -- a categorization that reflects a functionalist philosophy that was prevalent in Riley's laboratory and that many of his students absorbed. According to this philosophy, it is acceptable to hypothesize that an animal might engage in complex processing of information, as long as one can operationalize evidence for such a process and the hypothesis can be presented in the context of testable predictions that can differentiate it from other mechanisms. The contributions to this volume represent the three most important areas of research in animal cognition -- stimulus representation, memory processes, and perceptual processes -- although current research has considerably blurred these distinctions.
Animal Colors
by Carrie Smith Lori O'DeaAnimals come in many colors. Red, blue, pink, green! Explore the wonderful world of animal colors!
Animal Communication
by Abbie DunneSimple text and bright photographs explain the concept of animal communication for beginning readers. The book concludes with a simple, kid-friendly activity.
Animal Communication Made Easy: Strengthen Your Bond and Deepen Your Connection with Animals
by Pea HorselyA practical and inspiring introductory guide to communicating with pets and wild animals.Your step-by-step guide to forming a deeper connection with animals.Do you love animals but wish you could understand what they're trying to tell you? Do some of their behaviours leave you baffled?In this book, world-renowned animal communicator Pea Horsley teaches you the essentials of animal communication to enable you to communicate intuitively with the animals you love. Pea leads you through grounding preparation processes to calm your body and release your mind, and then her effective five-step method to create a deep, spiritual connection with your animal.Drawing on her many years of experience teaching people to communicate with both wild and domesticated creatures, Pea's unique blend of exercises, affirmations and meditations will empower you to connect with all living beings.Communicating with animals is fun, profound and healing. It's the best thing you can do for both yourself and your animals, and will transform how you experience life.